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排序方式: 共有255条查询结果,搜索用时 15 毫秒
1.
Park SK An SJ Hwang IK Kim DW Jung JY Won MH Choi SY Kwon OS Jeong YG Kang TC 《Neuroscience research》2004,49(4):405-416
The present study was performed to determine whether the effects induced by GABA(B) receptor-acting drugs would be related with the alteration in GABA(B) receptor expression in the hippocampus using Mongolian gerbil, a genetic epilepsy model. The distribution patterns of both GABA(B) receptor 1A/B and GABA(B)receptor 2 immunoreactivities were similarly detected in the hippocampi of normal and seizure-prone gerbils. Following baclofen (GABA(B) receptor agonist) or phaclofen (GABA(B) receptor antagonist) treatment, GABA(B) receptor immunoreactivities were decreased or increased by dose-dependent manners, respectively. Vigabatrin (GABA transaminase inhibitor) or 3-mercaptopropionic acid (GAD inhibitor) treatment did not affect GABA(B) receptor expressions. These findings suggest that GABA(B) receptor expression in the gerbil hippocampus may be altered by baclofen or phaclofen treatment. 相似文献
2.
Differentiated HL-60 is an effector cell widely used for the opsonophagocytic-killing assay (OPKA) to measure efficacy of pneumococcal vaccines. We investigated the correlation between phenotypic expression of immunoreceptors and phagocytic ability of HL-60 cells differentiated with N,N-dimethylformamide (DMF), all-trans retinoic acid (ATRA), or 1α, 25-dihydroxyvitamin D3 (VitD3) for 5 days. Phenotypic change was examined by flow cytometry with specific antibodies to CD11c, CD14, CD18, CD32, and CD64. Apoptosis was determined by flow cytometry using 7-aminoactinomycin D. Function was evaluated by a standard OPKA against serotype 19F and chemiluminescence-based respiratory burst assay. The expression of CD11c and CD14 gradually increased upon exposure to all three agents, while CD14 expression increased abruptly after VitD3. The expression of CD18, CD32, and CD64 increased during differentiation with all three agents. Apoptosis remained less than 10% until day 3 but increased after differentiation by DMF or ATRA. Differentiation with ATRA or VitD3 increased the respiratory burst after day 4. DMF differentiation showed a high OPKA titer at day 1 which sustained thereafter while ATRA or VitD3-differentiated cells gradually increased. Pearson analysis between the phenotypic changes and OPKA titers suggests that CD11c might be a useful differentiation marker for HL-60 cells for use in pneumococcal OPKA.
Graphical Abstract
相似文献3.
Ahn So-Hyeon Choi Nam-Kyong Kim Ye-Jee Seong Jong-Mi Shin Ju-Young Jung Sun-Young Park Byung-Joo 《Archives of pharmacal research》2015,38(6):1255-1262
Archives of Pharmacal Research - This study examined 1-year persistency with cholinesterase inhibitors (ChEIs) for the treatment of elderly Alzheimer’s dementia (AD) patients in Korea. Korean... 相似文献
4.
We used a dual-chamber culture system separated by a dialysis membrane to test the efficiency of expansion of whole cord blood and cell fractions. We found that expansion of progenitor cells was more efficient from whole blood than from purified CD34+ or partially purified mononuclear cell fractions. 相似文献
5.
Ryu KH Cho SJ Jung YJ Seoh JY Kie JH Koh SH Kang HJ Ahn HS Shin HY 《International journal of hematology》2004,80(3):281-286
Dendritic cells (DCs) are the most potent antigen-presenting cells in terms of initiating primary T-cell-dependent immune responses. We devised a 2-step culture method for obtaining sufficient numbers of functional DCs from umbilical cord blood (CB) CD34+ cells. In the first step, CB CD34+ cells were expanded by stimulation with early-acting cytokines such as stem cell factor (SCF), flt3 ligand (FL), and thrombopoietin (TPO) to amplify the hematopoietic progenitor cells. In the second step, granulocyte-macrophage colony-stimulating factor and interleukin 4 were added, and incubation was continued for another 5 days to induce differentiation of the expanded cells into DCs. During the first step of culturing with TPO, SCF, and FL, the total numbers of nucleated cells gradually increased, peaking at 4 weeks (245.3-fold). During the second step, expression of CD1a, CD83, and CD86 increased. Electron microscopic findings showed that these cells had cytosolic expansion to form dendrites and major histocompatibility complex class II compartments, which are characteristic of DCs. Functional analyses revealed that these cells had phagocytic activity and were capable of stimulating allogeneic T-cells in vitro. 相似文献
6.
Hyung‐Ran Kim Su‐Min Lee Jong‐Wha Won Woosung Lim Byung‐In Moon Hyun‐Jong Yang Ju‐Young Seoh 《Immunology》2013,138(1):57-67
Regulatory T (Treg) cells are important in the regulation of immune response, but the exact regulation of Treg‐cell function in vivo is still not well known. In the present study, we investigated the functional activity of CD4+ CD25+ Treg cells as well as the frequency and number of CD4+ CD25+ FoxP3+ Treg cells in the spleens of experimentally infected mice with a tissue‐migrating parasite, sparganum (plerocercoid of Spirometra mansoni) for 3 weeks. The results demonstrated fluctuations in the Treg‐cell function during the parasite infection, being up‐regulated at day 3, down‐regulated until day 14, and thereafter up‐regulated again at day 21. We also investigated the cytokine‐producing capability of the splenocytes to study the pattern of immune response of the mice to the parasite. The results showed decreased capabilities of interleukin‐2 (IL‐2), interferon‐γ (IFN‐γ) and IL‐17α production, whereas IL‐4‐producing and IL‐10‐producing capabilities were increased along with the parasitic infection. Meanwhile, IL‐6‐producing capability was increased to reach a peak at week 2, and thereafter was decreased to the baseline level. As a regulatory mechanism, we found that Treg‐cell function was attenuated in the presence of the crude extracts of sparganum, but was enhanced in the presence of the excretory–secretory products, suggesting that sparganum products were involved in the triggering and regulation of immune response in the acute and chronic phases, respectively. Results show that Treg cells are central in the immune homeostasis in vivo that is maintained by host–parasite interactions during the parasitic infection. 相似文献
7.
8.
Byoung-Sun Park Ju-Hyun Kim Mee-Young Kim Lim-Kyu Lee Seung-Min Yang Hye-Joo Jeon Won-Deok Lee Ji-Woong Noh Jeong-Uk Lee Taek-Yong Kwak Tae-Hyun Lee Ju-Young Kim Junghwan Kim 《Journal of Physical Therapy Science》2015,27(3):641-644
[Purpose] The purpose of this study was to investigate the effects of strengthening
exercises for the hip extensors on the gait performance and stability of patients with
hemiplegia. [Subjects and Methods] The subjects were fifteen stroke patients (ten males,
five females). The experimental subjects performed a hip extensor strengthening exercise
(HESE) program for a total of four weeks. [Results] The experimental subjects showed
significant improvements after the HESE program. Especially, walking speed and the
affected side stance phase time significantly increased after the HESE program.
Furthermore, the affected side stride length and symmetry index in the stance phase
significantly increased after HESE program. [Conclusion] These results suggest that the
HESE program may, in part, help to improve gait performance ability and stabilize physical
disability after stroke.Key words: Stroke patients, Hip extensor strengthening exercise (HESE) program, Gait function 相似文献
9.
Byoung-Sun Park Mee-Young Kim Lim-Kyu Lee Seung-Min Yang Won-Deok Lee Ji-Woong Noh Yong-Sub Shin Ju-Hyun Kim Jeong-Uk Lee Taek-Yong Kwak Tae-Hyun Lee Ju-Young Kim Junghwan Kim 《Journal of Physical Therapy Science》2015,27(5):1603-1607
[Purpose] The purpose of this study was to confirm the effects of both conventional
overground gait training (CGT) and a gait trainer with partial body weight support (GTBWS)
on spatiotemporal gait parameters of patients with hemiparesis following chronic stroke.
[Subjects and Methods] Thirty stroke patients were alternately assigned to one of two
treatment groups, and both groups underwent CGT and GTBWS. [Results] The functional
ambulation classification on the affected side improved significantly in the CGT and GTBWS
groups. Walking speed also improved significantly in both groups. [Conclusion] These
results suggest that the GTBWS in company with CGT may be, in part, an effective method of
gait training for restoring gait ability in patients after a stroke.Key words: Gait training, Spatiotemporal gait parameters, Stroke patients 相似文献
10.
Ji-Woong Noh Byoung-Sun Park Mee-Young Kim Lim-Kyu Lee Seung-Min Yang Won-Deok Lee Yong-Sub Shin Ju-Hyun Kim Jeong-Uk Lee Taek-Yong Kwak Tae-Hyun Lee Ju-Young Kim Jaehong Park Junghwan Kim 《Journal of Physical Therapy Science》2015,27(10):3223-3226
[Purpose] The purpose of the present study was to elucidate the muscle conditions such as
the isokinetic muscle of Korean ssireum athletes. [Subjects and Methods] This study
enrolled 25 elite ssireum athletes. We measured body composition and peak torque at an
angular speed at 60°/s using an isokinetic muscle strength dynamometer. [Results] The lean
body mass of the left upper limb was significantly higher than that of the right upper
limb. However, the lean body mass of the left lower limb was significantly lower than that
of the right lower limb. The peak torque for left elbow flexion was significantly higher
than that for right elbow flexion. Conversely, the peak torque for left elbow extension
was significantly lower than that for right elbow extension. Furthermore, the peak torque
for the left knee was significantly lower than that for the right knee for both flexion
and extension. [Conclusion] The data from this study elucidate in part the muscle
conditions of Korean ssireum athletes, which can be used to establish a reference for the
scientific study of sports physiotherapy.Key words: Isokinetic muscle strength, Ssireum athletes, Sports physiotherapy 相似文献