Tumor necrosis factor (TNF) is a cytokine that possesses many biological activities, including enhancement of non-rapid-eye-movement sleep (NREMS). The role of endogenous TNF in the regulation of spontaneous sleep is unknown. If TNF is involved in sleep regulation, then reduction of endogenous TNF should suppress spontaneous sleep. A soluble TNF-binding protein I (TNF-BP I) and a synthetic fragment of TNF-BP I, TNF-R-(159–178), that contains the biologically active region of TNF-BP I, were used. These substances bind TNF and possess TNF-inhibitory activity; their effects on rabbit sleep after intracerebroventricular injection were determined across a 6-h recording period. Two doses of TNF-BP I (0.05 g and 0.5 g) were administered; the higher dose of TNF-BP I significantly decreased NREMS. Four doses of TNF-R-(159–178) (0.25 g, 2.5 g, 25 g and 50 g) were used. The 25 g and 50 g doses significantly suppressed NREMS. The highest dose (50 g) also decreased REM sleep. These results are consistent with the hypothesis that endogenous brain TNF is involved in the regulation of normal sleep. 相似文献
One-step separation and recovery of sodium, aluminum and iron in high-iron red mud in a high-calcium alkaline hydrothermal system is realized by a high-pressure hydrothermal reduction process. The transformation behavior of the aluminasilica phase in high-iron red mud is mainly investigated. The results show that under the optimized conditions, a temperature of 290℃, a Na2O concentration of 240 g/L, a calcium to silicon ratio of 3.5, and a liquid–solid ratio of 5, the Na2O content in the transition slag is reduced to 0.12%, the dealkalization rate can reach 98%, and the alumina dissolution rate is 73%. When the starch-free reductant is added, the transition slag mainly consists of hematite and hydroandradite, and when the starch reductant is added (the addition amount is 1/4 that of ω(Fe2O3) in the red mud), all Fe2O3 in the transition slag is completely reduced to Fe3O4, and the main phases are magnetite and hydrogrossular.