全文获取类型
收费全文 | 615篇 |
免费 | 63篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 16篇 |
儿科学 | 12篇 |
妇产科学 | 3篇 |
基础医学 | 84篇 |
口腔科学 | 11篇 |
临床医学 | 48篇 |
内科学 | 154篇 |
皮肤病学 | 10篇 |
神经病学 | 23篇 |
特种医学 | 12篇 |
外国民族医学 | 1篇 |
外科学 | 148篇 |
综合类 | 11篇 |
预防医学 | 26篇 |
眼科学 | 25篇 |
药学 | 52篇 |
中国医学 | 5篇 |
肿瘤学 | 41篇 |
出版年
2023年 | 14篇 |
2022年 | 28篇 |
2021年 | 31篇 |
2020年 | 19篇 |
2019年 | 15篇 |
2018年 | 14篇 |
2017年 | 17篇 |
2016年 | 25篇 |
2015年 | 19篇 |
2014年 | 25篇 |
2013年 | 32篇 |
2012年 | 46篇 |
2011年 | 58篇 |
2010年 | 28篇 |
2009年 | 19篇 |
2008年 | 39篇 |
2007年 | 32篇 |
2006年 | 32篇 |
2005年 | 35篇 |
2004年 | 23篇 |
2003年 | 29篇 |
2002年 | 19篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 6篇 |
1997年 | 2篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 8篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 6篇 |
排序方式: 共有682条查询结果,搜索用时 33 毫秒
1.
2.
A new dimeric indoline alkaloid has been isolated from the leaves of PETCHIA CEYLANICA. Its structure has been assigned as 1 on the basis of spectral studies. Its stereochemistry has been established by NOE difference measurements. 相似文献
3.
A C-Terminal Hydrophobic Region is Required for Homo-Oligomerization of the Hepatitis E Virus Capsid (ORF2) Protein 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Journal of biomedicine & biotechnology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Hepatitis E virus (HEV) is the causative agent of hepatitis E, anacute form of viral hepatitis. The open reading frame 2 (ORF2) of HEV encodes the viral capsid protein, which can self-oligomerize into virus-like particles. To understand the domains within this protein important for capsid biogenesis, we have carried out in vitro analyses of association and folding patterns of wild type and mutant ORF2 proteins. When expressed in vitro or in transfected cells, the ORF2protein assembled as dimers, trimers and higher order forms. WhileN-terminal deletions up to 111 amino acids had no effect, the deletion of amino acids 585–610 led to reduced homo-oligomerization. This deletion also resulted in aberrant folding of the protein, as determined by its sensitivity to trypsin. This study suggests that a C-terminal hydrophobic region encompassing amino acids 585–610 of the ORF2 protein might be critical for capsid biogenesis. 相似文献
4.
Timothy A. Jennings MD Ng Bernard MD Ann Boguniewicz MD Muzaffar Khan MD Donald Rice MD James Figge MD 《Endocrine pathology》1998,9(4):353-361
Hemorrhagic adrenal pseudocysts are uncommon nonneoplastic lesions that have been reported as secondary to intraparenchymal
hemorrhage or alternatively related to endothelial (vascular) cysts. Ultrastructural and immunohistochemical evidence in support
of the latter has been presented, but the exact nature of hemorrhagic adrenal pseudocysts remains poorly defined. We evaluated
six surgical specimens of hemorrhagic adrenal pseudocysts using immunohistochemical staining for CD31 and CD34, as well as
conventional histochemistry. All six cases had hemorrhagic contents within a wall of variable thickness possessing focal areas
of linear, disrupted elastin, and smooth muscle. Three cases demonstrated extensive thrombosis with organization, including
papillary endothelial hyperplasia, simulating angiosarcoma. In these cases, CD31 and CD34 staining decorated areas of papillary
endothelial hyperplasia as well as foci of the internal cyst lining, whereas the other cases were negative for both antibodies.
Of interest is the history of FNA prior to surgical resection in three cases of hemorrhagic adrenal pseudocysts, two of which
showed papillary endothelial hyperplasia. The presence of papillary endothelial hyperplasia and our immunohistochemical findings
support, the conclusion that adrenal pseudocysts are posthemorrhagic and derive from vascular disruption. Furthermore, FNA
or other interventional studies may be associated with papillary endothelial hyperplasia in hemorrhagic adrenal pseudocysts. 相似文献
5.
Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes 总被引:4,自引:0,他引:4
DNA vaccines encoding a viral structural protein have been shown to induce antiviral immune responses and provide protection against subsequent viral challenge. In the present study we show that DNA immunization with a plasmid expressing the hepatitis E virus ORF2 structural protein (pcDNA-ORF2) induced low levels of long-lasting antibody responses in the murine model. The use of plasmids expressing interleukin-2 (IL-2) and granulocyte-macrophage colony-stimulating-factor (GM-CSF) in conjunction with pcDNA-ORF2 enhanced the antibody responses generated by pORF-2. We further show that the immune responses generated by plasmid pcDNA-ORF2 can be boosted with virus-like particles composed of the ORF2 protein expressed through a baculovirus expression system. 相似文献
6.
Studies on the alkaloidal constituents of the leaves of ERVATAMIA CORONARIA Stapf and ALSTONIA SCHOLARIS have resulted in the isolation of (20 S)-19,20-dihydrocondylocarpine which has not been previously reported from these plants. The stereochemistry at C-20 was established with the help of n. O. e. difference measurements. (13)C-NMR assignments have been made by polarisation transfer techniques. 相似文献
7.
8.
Outcome of patients with systemic light chain amyloidosis with concurrent renal and cardiac involvement
下载免费PDF全文
![点击此处可从《European journal of haematology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Talha Badar Amanda Megan Cornelison Nina D. Shah Qaiser Bashir Simrit Parmar Krina Patel Chitra Hosing Uday Popat Donna M. Weber Sheeba K. Thomas Jatin J. Shah Robert Z. Orlowski Richard E. Champlin Muzaffar H. Qazilbash 《European journal of haematology》2016,97(4):342-347
Cardiac involvement in systemic light chain amyloidosis (AL) is generally associated with a worse outcome, especially if other organs are also involved. We sought to determine whether concurrent cardiac and renal involvement were associated with a worse outcome than either organ alone. We identified 129 patients with AL, who received high‐dose chemotherapy followed by autologous hematopoietic stem cell transplantation (auto‐HCT) at our institution between 1997 and 2014. Ninety‐nine patients had either renal (group 1: n = 62, 62%), cardiac (group 2: n = 20, 20%), or both cardiac and renal (group 3: n = 17, 17%) involvement. The overall hematological response rate (CR+VGPR+PR) post‐auto‐HCT in groups 1, 2, and 3 was 69%, 74% and 82%, respectively (P = 0.62). Overall, organ response in groups 1, 2, and 3 was 39%, 42%, and 70%, respectively. The median PFS from auto‐HCT in groups 1, 2, and 3 was not reached (NR), 13.3 and 21 months, respectively (P = 0.02). The median OS in groups 1, 2, and 3 was 120, 46, and 60 months, respectively (P = 0.1). In conclusion, median PFS and OS in patients with concurrent cardiac and renal AL were comparable to patients with cardiac AL only, but worse than patients with renal AL. 相似文献
9.
Arif Zafar Gemma Ingham Jainudeen K.A. Jameel 《International journal of surgery case reports》2013,4(5):528-530
INTRODUCTIONBouveret's syndrome is a rare variant of gallstone ileus and describes gastric outlet obstruction secondary to an impacted stone in the duodenum. Its presentation is vague and clinical diagnosis is often difficult resulting in a delay in diagnosis.PRESENTATION OF CASEWe report a patient who presented initially with non-specific symptoms and subsequently with features in keeping with acute pancreatitis, but eventually was found to have Bouveret's syndrome.DISCUSSIONDifferent treatment strategies are discussed. Although endoscopic treatment combined with many newer modalities like lithotripsy have been tried, surgery remains the definitive management in the vast majority of cases.CONCLUSIONBouveret's syndrome is a rare condition, can also present as pancreatitis and often difficult to diagnose initially, but with appropriate treatment has a good outcome. 相似文献
10.
Ahmad S. Azhar Zaher F. Zaher Noran M. Abu-Ouf Jameel Al-Ata Sara Taisir A. Alsheblie 《Journal of the Saudi Heart Association》2018,30(1):28-39