a-1, 2岩藻糖基转移酶II在肺鳞癌组织中的表达及对肺鳞癌细胞增殖的影响

目的：探讨a-1，2岩藻糖基转移酶II（FUT2）在肺鳞癌中的表达情况及其对肺鳞癌细胞增殖的影响。方法：应用UALCAN数据库分析FUT2在临床肺鳞癌患者组织中的差异表达及其与肺鳞癌患者临床TNM分期、年龄及性别因素的相关性；采用瞬时转染技术，将FUT2的shRNA质粒转染进H226细胞株中构建FUT2低表达的肺鳞癌细胞模型；利用CCK-8、平板克隆及Western blot实验检测FUT2对肺鳞癌细胞的生长、单克隆细胞团形成及PCNA蛋白表达的影响。结果：FUT2在肺鳞癌尤其是肺鳞癌TNM早中期呈现高表达趋势（P＜0.05），而与年龄和性别无关。低表达FUT2可以抑制肺鳞癌细胞的生长速率和单克隆细胞团的形成数，同时，可以抑制肺鳞癌细胞PCNA蛋白的表达水平，差异均有统计学意义（P＜0.05）。结论：FUT2在肺鳞癌中高表达且促进肺鳞癌细胞的增殖能力，有望成为肺鳞癌的早期诊断指标。     

济南市2020年蚊类监测结果及优势蚊种抗药性检测结果分析

目的 了解2020年济南市蚊类种群组成、密度及季节消长规律;了解济南市优势蚊种的抗药性情况,为病媒生物防控工作提供科学依据.方法 蚊虫监测采用诱蚊灯法,监测3至11月份;优势蚊种抗药性检测,采用浸渍法、点滴法等.结果 蚊类,平均密度为6.15只/(灯·夜),以淡色库蚊为主,猪圈的蚊类密度最高;优势蚊种抗药性检测,对常用菊酯类药剂均有不同程度抗药性.结论 掌握了2020年济南市蚊类种群组成、密度及季节消长规律及优势蚊种抗药性情况,为济南市及济南口岸蚊类的防治工作提供理论依据.     

淫羊藿甙抗癌作用机制的实验研究

中药单体淫羊藿百一种新型的生物反应调节剂和诱导分化剂。为进一步探讨淫羊藿 作用机制,我们将淫羊藿和于人高转移肺癌细胞ＰＧ,采用ＭＴＴ、放免、流式细胞术、粘附实验,运动侵袭实验等多种手段进行了检测。结果表明,淫羊藿夼直接抑癌作用较强,但却能影响ＰＧ细胞周期的时相分布,使Ｓ期减小,并能升高细胞内ｃ－ＡＭＰ水平,降低ｃＧＭＰ水平,提示ｃＡＭＰ／ｃＧＭＰ比值,同时降低ＰＧ细胞对胞外基质的粘附性及侵袭、运动     

原发性肝癌合并2型糖尿病患者临床病理特点的比较性研究

目的:对比分析原发性肝癌合并2型糖尿病患者的临床病理特点,为临床治疗提供一定治疗依据.方法:回顾性分析手术切除治疗且经病理确诊的原发性肝癌患者资料,比较合并2型糖尿病(T2DM组)与无合并2型糖尿病患者(无T2DM组)的临床病理特点,分析患者的性别、年龄、教育程度、吸烟、饮酒、BMI、Child-Pugh分级、实验室检查、病理TNM分期等的异同.结果:T2DM组的男性患者比例高于无T2DM组.T2DM组患者的年龄为(58.07 ±9.77)岁,高于无T2DM组(51.76±11.22)岁,P＜0.001.入院时的收缩压、舒张压、BMI、伴有慢性病高血压患者也高于无T2DM组(P＜0.05).两组间肝癌都以中分化肝细胞癌为主,但所占比例有统计学差异,T2DM组高分化所占比例高于无T2DM组.而在肝硬化、乙肝、AFP值中却是无T2DM组高于T2DM组.吸烟、饮酒、教育程度、肝功实验室检查、Child-Pugh分级、TNM分期等在两组间无明显统计学意义.结论:2型糖尿病可能加大肝癌的发生率,尤其是老年男性患者,建议肝癌患者应注意筛查2型糖尿病或糖尿病前期,关注肝癌合并2型糖尿病患者的治疗.     

SCR7对三阴性乳腺癌顺铂化疗的增敏作用研究

目的：探讨SCR7对三阴性乳腺癌顺铂化疗的增敏作用。方法：选用三阴乳腺癌MDA-MB-231细胞分为空白对照组、10μmol/L顺铂对照组和联合组（10μmol/L顺铂+10、20和40μmol/L SCR7）,采用CCK-8法检测细胞活性并计算细胞增殖抑制率;取对数期TNBC MDA-MB-231细胞构建三阴性乳腺癌移植瘤小鼠模型,建模成功后荷瘤裸鼠随机分为对照组、SCR7组,顺铂组、SCR7+顺铂组;饲养24 d后,观察比较各组荷瘤鼠肿瘤质量、瘤体体积的变化,计算肿瘤抑制率;苏木精-伊红（hematoxylin-eosin,HE）染色观察瘤体组织细胞形态学变化;原位末端标记法（in situ end labeling,TUNEL）检测乳腺癌肿瘤组织的凋亡;采用Western blot检测X射线修复交叉互补蛋白1(X-ray repair cross complementing 1,XRCC1)、多聚（ADP核糖）聚合酶1[poly(adp ribose) polymerase 1,PARP1]蛋白表达。结果：联合组细胞增殖抑制率均高于对照组（P<0.05）,且随SCR7质量浓...     

Interstitial nephritis without glomerulonephritis in ANCA-associated vasculitis: a case series and literature review

Clinical Rheumatology - The typical nephrological presentation of antineutrophil cytoplasmic antibody (ANCA)–associated vasculitis (AAV) is rapidly progressive glomerulonephritis....     

Cautions on the laboratory indicators of COVID‐19 patients on and during admission

BackgroundDifferent disease severities of COVID‐19 patients could be reflected on clinical laboratory findings.MethodsIn this single‐centered retrospective study, demographic, clinical, and laboratory indicators on and during admission were compared among 74 participants with mild, moderate, critical severe, or severe classification. Risk factors associated with disease severity were analyzed by multivariate analyses. The AUC and 95% CI of the ROC curve were calculated.ResultsThe most common manifestations of these patients were fever and cough. Critical severe or severe group owned the longest length of stay (23 (19,31), p < 0.001). After multivariate logistic regression, independent influence factors on admission for severity of disease were CK‐MB (OR 0.674; 95% CI 0.489–0.928; p = 0.016), LDH (OR 1.111 or 1.107; 95% CI 1.026–1.204 or 1.022–1.199; p = 0.009 or 0.013), normal T‐BIL (OR 4.58 × 10⁻⁸; 95% CI 3.05 × 10⁻⁹–6.88 × 10⁻⁷; p < 0.001), LYM% (OR 0.008; 95% CI 0–0.602; p = 0.029), and normal ESR (OR 0.016; 95% CI 0–0.498; p = 0.019). Factors during hospitalization were normal T‐BIL (OR 8.56 × 10⁻⁹; 95% CI 8.30 × 10⁻¹⁰–8.83 × 10⁻⁸; p < 0.001), LYM (OR 0.068; 95% CI 0.005–0.934; p = 0.044), albumin (OR 0.565; 95% CI 0.327–0.977; p = 0.041), and normal NEU% (OR 0.013; 95% CI 0.000–0.967; p = 0.048). Combined indicators of AUC were 0.860 (LYM, LDH, and normal ESR on admission, p < 0.001) and 0.750 (CK‐MB, LDH, and normal T‐BIL during hospitalization, p = 0.020) when predicting for severe or critical severe patients.ConclusionTo pay close attention to the progression of COVID‐19 and take measures promptly, we should be cautious of the laboratory indicators when patients on admission especially CK‐MB, LDH, LYM%, T‐BIL as well as ESR; and T‐BIL, LYM, albumin, NEU% with the process of disease.     

Synthesis of tetrahydro-β-carbolines from 2-indolylmethyl azides and propargylic alcohols

A facile and efficient route to tetrahydro-β-carbolines from 2-indolylmethyl azides and propargylic alcohols via acid-catalyzed dehydrative annulation reactions is described. This reaction proceeds through a cascade sequence of Friedel–Crafts-type alkylation followed by intramolecular “Click” reaction, involving the formation of multiple chemical bonds in a single operation with excellent atom-economy and broad functional group tolerance.

The synthesis of tetrahydro-β-carbolines from 2-indolylmethyl azides and propargylic alcohols via acid-catalyzed dehydrative [4 + 2]-annulation reaction is described.