Inframalleolar bypass in limb salvage. Late results

STUDY OBJECTIVE: The objective of this retrospective study was to report the long-term results of distal revascularization at the ankle in patients with critical ischemia. PATIENTS AND METHODS: From January 1989 to November 1999, 50 inframalleolar bypasses were performed in 49 patients (35 males and 14 females with a mean age of 75 years [range: 51 to 95 years]). Twenty-five patients (50%) were diabetics. All patients presented with critical ischemia of the lower limb. Distal anastomosis was performed at the retromalleolar posterior tibial artery in 28 cases (56%), pedal artery in 20 cases (40%) and distal fibular artery in two cases (4%). Bypasses were performed using a greater saphenous vein (n = 38), a cryopreserved arterial allograft (n = 9), or a PTFE graft (n = 2). A composite graft with greater saphenous vein and arterial allograft was performed in two cases. RESULTS: There were two early postoperative deaths. One patient presented a blow-out of the distal anastomosis that required bypass ligation and subsequent leg amputation. Early thrombosis of the graft occurred in four cases, leading to major amputation in three cases. No patients were lost to follow-up and mean follow-up was 26.7 months (range: 1 to 86 months). Graft thrombosis occurred in 15 patients and led to amputation in 6 cases. Bypass graft patency rate was 72% and 61% at 1 year and 3 years, respectively, yielding a 80% limb salvage rate at 3 years. The 3-year actuarial survival rate was 53%. CONCLUSION: Inframalleolar bypasses are a valuable tool in patients with critical ischemia. The lower limb salvage rate is satisfactory in this elderly population. Based on this experience, angiography with good run-off and, when necessary, a surgical approach to verify patency of the arteries at the ankle should always be performed before undertaking a major amputation.     

Partial blockage of the inferior vena cava. Results apropos of 110 cases]

Rheoplethysmography with occlusion (RPO) is useful in the diagnosis of deep venous thrombosis. Bilateral phlebography with cavography performed afterwards as an emergency gives information as to the embolic potential of the thrombosis and orientates treatment towards surgical interruption of the inferior vena cava. In the C.H.U. at Grenoble 83 clips, 25 umbrella filters and 2 ligatures were placed on the inferior vena cava from 1974 to 1977. Prevention of pulmonary emboli was achieved in 99% cases with lower limb embolic foci. Follow-up by RPO and isotopic phlebography showed that the course of venous thrombosis of the lower limbs seemed to be unchanged by partial interruption of the inferior vena cava, provided that elastic stockings and adequate anticoagulant therapy adapted to venous haemodynamics are used.     

Isolation of 10 differentially expressed cDNAs in p53-induced apoptosis: activation of the vertebrate homologue of the drosophila seven in absentia gene.

We report the isolation of 10 differentially expressed cDNAs in the process of apoptosis induced by the p53 tamor suppressor. As a global analytical method, we performed a differential display of mRNA between mouse M1 myeloid leukemia cells and derived clone LTR6 cells, which contain a stably transfected temperature-sensitive mutant of p53. At 32 degrees C wild-type p53 function is activated in LTR6 cells, resulting in programmed cell death. Eight genes are activated (TSAP; tumor suppressor activated pathway), and two are inhibited (TSIP, tumor suppressor inhibited pathway) in their expression. None of the 10 sequences has hitherto been recognized as part of the p53 signaling pathway. Three TSAPs are homologous to known genes. TSAP1 corresponds to phospholipase C beta 4. TSAP2 has a conserved domain homologous to a multiple endocrine neoplasia I (ZFM1) candidate gene. TSAP3 is the mouse homologue of the Drosophila seven in absentia gene. These data provide novel molecules involved in the pathway of wild-type p53 activation. They establish a functional link between a homologue of a conserved developmental Drosophila gene and signal transduction in tumor suppression leading to programmed cell death.     

24-hour extracorporeal membrane oxygenation in the hypoxic dog: hemodynamics and pulmonary gas exchange.

Hemodynamic and pulmonary gas exchange values were investigated during 24-hour extracorporeal membrane oxygenation performed on 7 anesthetized dogs subjected to alveolar hypoxia. The chief effects of extracorporeal membrane oxygenation were demonstrated. The duration of the extracorporeal membrane oxygenation was associated with a progressive decline in the systemic arterial pressure and heart rate and a progressive increase in the pulmonary blood flow rate. Left-ventricular stroke work remained constant. The sum of the pulmonary and extracorporeal oxygen uptakes showed no change in spite of large variations in pulmonary flow rate.     

Surgical techniques used for the treatment of varicose veins: survey of practice in France

This survey was undertaken in 2001 among surgeons of the French speaking Vascular Surgery Society (SCV-Société de Chirurgie Vasculaire de Langue Fran?aise) and SCV non-members with a heavy caseload in varicose vein surgery. AIM OF THE STUDY: To identify --the various surgical procedures used for treating chronic venous disease and in particular varicose veins; --the current use of preoperative investigation with duplex ultrasound; --the type of anesthesia used; --the postoperative treatment prescribed, and specifically compression therapy. MATERIAL AND METHODS: This survey was conducted by mail through a "half open" questionnaire including 17 questions and a patient form (see appendices I and II). 675 surgeons were questioned (501 were SCV members and 174 non members). RESULTS: Two-hundred and eighty surgeons answered (41.5%). The level of replies for French surgeons was 45.3%. A scientific committee assessed these replies. The surgeons were classified into different groups according to their membership of the SCV, own practice, and caseload. The 2 most performed procedures were respectively high ligation + saphenous trunk stripping + tributaries stab avulsion (71.9%) and high ligation + saphenous trunk stripping (17.3%). Isolated phlebectomy was 5.6%, high ligation + tributaries stab avulsion + saphenous trunk preservation 2.8%, isolated high ligation 2.2%, and ambulatory hemodynamic and conservative treatment of venous insufficiency (CHIVA) 0.3% ). The various procedures used (total number, average and percentage) inside the different groups are displayed in, and. Concerning trunk stripping modality the 2 most frequently used techniques were invagination and Babcock techniques. Both were evaluated respectively for the great saphenous vein (invagination 78.1%, Babcock technique 44.2%) and the small saphenous vein (invagination 77.1%, Babcock technique 31.8%). Complete resection of the saphenous trunk was more frequently performed than partial stripping. Pre-operative duplex scanning was systematically undertaken by 85.4% of surgeons without a statistical difference between the different groups. General anesthesia remains the most used form of anesthesia (83.9%) followed by spinal or epidural anesthesia (70.4%), and local or loco-regional (29.2%). As multiple answers were allowed, the most frequent procedures associated with general anesthesia were spinal or epidural (36.9%). Responders prescribed postoperative compression and anticoagulation in 97.1% and 55.8% respectively. Intra-group comparison was then undertaken in order to determine if their practice was different. Annual caseload was significantly (P=0.001) higher in Group I (353) than in Group II (226) and Group III (152). There was no difference in terms of the various surgical procedures used between the different groups. Group 1 favored the trunk stripping modality invagination for avulsion of the great saphenous vein and small saphenous vein. Concerning anesthesia, local and loco-regional anesthesia was used more by Group I than by the other groups as was anticoagulation. Responders perform perforator ligation and deep venous reconstructive surgery respectively in 70.3% and 22.4% without any intra group difference, however we have no information on frequency and indications for this type of surgery as these items were not included in the questionnaire. DISCUSSION: Total number of procedures recorded in this survey is in keeping with the yearly French data concerning surgical treatment of varicose veins (ie, approximately 200,000 procedures). Since the emphasis was on surgical procedures sparing the saphenous trunks in varicose vein treatment only 10.9% of the techniques used in this survey were compatible with this purpose. Invagination technique for stripping both the great and small saphenous trunk was preferentially used by the Group I. Although it is surprising that all groups favored total trunk stripping particularly for the small saphenous vein. It is not surprising that a large majority, despite very little controversy on this point, performed preoperative duplex scanning. Although postoperative compression can be only quoted as a grade C recommendation according to evidence-based medicine, it was prescribed in almost all cases. CONCLUSION: Surgery for varicose veins is one of the most frequently performed surgical procedures in France, mainly by surgeons exercising in private practice. There is no significant difference between the various groups (SCV Member or not, type of practice and annual case load) concerning the various procedures used, although through careful analysis certain different tendencies may be identified. In addition postoperative compression is systematically prescribed.     

Evaluation of a clinical and scintigraphic management strategy for cardiac risk before abdominal aorta surgery. Apropos of 982 surgical patients

The incidence of major cardiac events (death, infarction) is over 5% after programmed aortic vascular surgery. The aim of this study was to evaluate a management strategy of this risk based on the clinical status and targeted indication of myocardial scintigraphy, coronary angiography and myocardial revascularisation. A first phase (1991-1993, 451 patients) confirmed the prognostic value of clinical (age, previous cardiac history, diabetes, hypertension, electrocardiogrammes) and scintigraphic features: the cardiac mortality was 1.25% in patients with a low clinical risk (70.3% of cases) and 4.5% in patients with a high clinical risk (2 factors, 29.3% of cases) (p < 0.01). In the latter group, the mortality was zero after normal myocardial scintigraphy and 7.2% after abnormal myocardial scintigraphy (p < 0.01) and 12.5% in cases with reversible defects (p < 0.01). During the second phase of the study (1994-1997, 531 patients) coronary angiography was performed in patients with a high clinical risk and abnormal scintigraphy (10.9% of cases). This led to a myocardial revascularisation in 3.6% of patients. The cardiac mortality was then the same in the low and high a priori clinical risk: 2.3 and 2.8% (NS). The use of simple clinical criteria enables surgery in the majority of candidates for aortic vascular surgery, scintigraphy being reserved for about one patient in ten with myocardial revascularisation in less than 4% of cases. The operative cardiac mortality then decreases to under 2.5%.     

Nutritional and genetic determinants of vitamin B and homocysteine metabolisms in neural tube defects: a multicenter case-control study

Neural tube defects (NTDs) are severe congenital malformations due to failure of neural tube formation in early pregnancy. The proof that folic acid prevents NTDs raises the question of whether other parts of homocysteine (Hcy) metabolism may affect rates of NTDs. This French case-control study covered: 77 women aged 17-42 years sampled prior to elective abortion for a severe NTDs (cases) and 61 women aged 20-43 years with a normal pregnancy. Plasma and erythrocyte folate, plasma B6, B12 and Hcy were tested as five polymorphisms MTHFR 677 C --> T, MTHFR 1298 A --> C, MTR 2756 A --> G, MTTR 66 A --> G and TCN2 776 C --> G. Cases had significantly lower erythrocyte folate, plasma folate, B12 and B6 concentrations than the controls, and higher Hcy concentration. The odds ratio was 2.15 (95% CI: 1.00-4.59) for women with the MTRR 66 A --> G allele and it was decreased for mothers carrying the MTHFR 1298 A --> C allele. In multivariate analysis, only the erythrocyte folate concentration (P = 0.005) and plasma B6 concentration (P = 0.020) were predictors. Red cell folate is the main determinant of NTDs in France. Folic acid supplement or flour fortification would prevent most cases. Increased consumption of vitamins B12 and B6 could contribute to the prevention of NTDs. Genetic polymorphisms played only a small role. Until folic acid fortification becomes mandatory, all women of reproductive age should consume folic acid in a multivitamin that also contains B12 and B6.