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1.
Ethylation of poly(dC-dG).poly(dC-dG) by ethyl methanesulfonate stimulates the activity of mammalian DNA methyltransferase in vitro. 总被引:6,自引:2,他引:4 下载免费PDF全文
I K Farrance R Ivarie 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(4):1045-1049
Ethylation of poly(dC-dG).poly(dC-dG) with ethyl methanesulfonate (EtMes), a known carcinogen, at increasing molar ratios of EtMes/C X G base pairs progressively stimulated the methyl-accepting ability of the DNA during in vitro methylation by partially purified rat DNA (cytosine-5)-methyltransferase (EC 2.1.1.37). Maximum stimulation was 2-fold over mock-treated DNA when 2.7% of the guanines were modified at the N-7 position, the major site of ethylation by EtMes in DNA. If a CpG site "hemiethylated" at guanine N-7 mimics a hemimethylated CpG site, we calculate that the enzyme has a relative affinity for hemiethylated CpG 18-fold above unmodified CpG. If ethylation of a dioxyphosphate oxygen of the phosphodiester bond is responsible for stimulation, the relative affinity could be much higher, up to 370-fold. 相似文献
2.
M Puvaneswary J Gani IK Kalnins 《Journal of Medical Imaging and Radiation Oncology》1998,42(4):367-369
Glomus vagale are rare vascular tumours of the paraganglion cells of the vagus nerve, and they usually occur in the carotid space. Tumours can be familial, multicentric, malignant but rarely hormonally active. A rare case is reported of glomus vagale presenting as a supraclavicular mass. 相似文献
3.
Rehan Ul Haq Prashant Modi IK Dhammi Anil K Jain Puneet Mishra 《Indian Journal of Orthopaedics》2013,47(3):302-306
Conjoint bicondylar Hoffa fracture is an extremely rare injury. Only one case has been reported previously in the pediatric age group. We describe this injury in a 17-year-old male who presented following a fall with direct impact on his semiflexed right knee. Plain radiographs were inadequate to define the exact pattern of injury. Computed tomographic (CT) scans demonstrated the coronal fracture involving both the femoral condyles which were joined by a bridge of intact bone. The patient was treated with open reduction and internal fixation using swashbuckler (modified anterior) approach. Union occurred within 3 months and at final followup (at 18 months) the patient had a good clinical outcome. The possible mechanism of injury is discussed. 相似文献
4.
Konstantin Musiychuk Natalie Stephenson Hong Bi Christine E. Farrance Goran Orozovic Maria Brodelius Peter Brodelius April Horsey Natalia Ugulava Abdel‐Moneim Shamloul Vadim Mett Shailaja Rabindran Stephen J. Streatfield Vidadi Yusibov 《Influenza and other respiratory viruses》2007,1(1):19-25
Historically, most vaccines have been based on killed or live‐attenuated infectious agents. Although very successful at immunizing populations against disease, both approaches raise safety concerns and often have limited production capacity. This has resulted in increased emphasis on the development of subunit vaccines. Several recombinant systems have been considered for subunit vaccine manufacture, including plants, which offer advantages both in cost and in scale of production. We have developed a plant expression system utilizing a ‘launch vector’, which combines the advantageous features of standard agrobacterial binary plasmids and plant viral vectors, to achieve high‐level target antigen expression in plants. As an additional feature, to aid in target expression, stability and purification, we have engineered a thermostable carrier molecule to which antigens are fused. We have applied this launch vector/carrier system to engineer and express target antigens from various pathogens, including, influenza A/Vietnam/04 (H5N1) virus. 相似文献
5.
6.
A significant recent advance that has occurred world over in the continuously evolving field of Magnetic Resonance Imaging (MRI) practice is the introduction of Cardiac applications. Cardiac MRI has moved to the centre stage of clinical management strategy by non-invasively imaging the structure as well as function of the heart. It has a wide range of specific applications such as delineation of morphological anatomy, quantification of flow and pressure across cardiac valve dysfunction, evaluation of myocardial function, assessment of infarcts, mapping coronary arteries and so on. Evaluation of congenital heart disease (CHD) is an important application of Cardiac MRI since the morphological details of chambers, septum, defects and anomalous connections are depicted accurately. Besides, flow information across valves, chambers, outflow tracts and shunts are also provided. This article describes our experience in the use of cardiac MRI in congenital heart disease.Key Words: Cardiac MRI, Congenital heart disease, Cyanotic and Acyanotic heart disease 相似文献
7.
8.
H. Yasemin BALABAN Abdullah Tark ASLAN efika AYAR Osman DA Alpaslan ALP Cem MEK Cavanir VAHABOV Tolga YILDIRIM Hakan G
KER Yahya BÜYÜKAIK Halis MEK 《Turkish Journal of Medical Sciences》2021,51(3):1229
Background/aim Hepatitis B virus (HBV) vaccination rates are insufficient in high-risk patients worldwide. This study aimed to investigate the screening, immunization, and vaccination rates in three high-risk groups for HBV infection: allogeneic hematopoietic stem cell transplantation (AHSCT), renal transplantation (RT), and chronic hepatitis C (CHC) groups. Materials and methods The serological data of consecutive patients between 2014 and 2019 were reviewed using the hospital database. Results The HBV screening rates were 100.0%, 90.4%, and 82.4% in the AHSCT, CHC, and RT groups, respectively (p = 0.003). The immunization rates against HBV through either previous exposure or vaccination were 79.5%, 71.7%, and 46.5% in the AHSCT, RT, and CHC groups, respectively (p < 0.001). The HBV vaccination rate was significantly low in the CHC group (71.5%, 69.0%, 34.6% in the AHSCT, RT, and CHC groups, respectively, p < 0.001). If patients lost their immunity due to immunosuppressive therapy were accounted, the vaccination rates increased to 95.2% in the AHSCT group and 72.9% in the RT group. The rate of annual screening for HBV status was 97.9% in the AHSCT group, but it was only 23.9% in the RT group. Conclusion HBV screening and vaccination rates were significantly lower in the RT and CHC groups than in the AHSCT group. 相似文献
9.
Paton NI; Cheong IK; Kong NC; Segasothy M 《QJM : monthly journal of the Association of Physicians》1996,89(7):531-538
To determine the incidence, types and risk factors for infection in
systemic lupus erythematosus (SLE) patients in Kuala Lumpur, Malaysia, we
retrospectively reviewed the medical records of 102 patients with definite
SLE attending a specialist clinic. Details of major infections (pneumonia
or severe infection requiring intravenous therapy) and minor infections,
and their time of onset in relation to immunosuppressive therapy and
disease flares were recorded. There were 77 major and 163 minor infections
during 564 patient-years of follow-up. In the month following a course of
pulse methylprednisolone, the incidence of major infection was 20 times
higher and the incidence of minor infection was 10 times higher than at
other periods (p<0.0001). In the month after disease flare, the
incidence of major infection was 10 times higher and the incidence of minor
infection six times higher than at other times (p<0.0001). After
allowing for methylprednisolone therapy and disease flares, there was no
increase in the rate of infections during treatment with azathioprine, oral
or intravenous cyclophosphamide. There was no effect of renal involvement
on infection rate.
相似文献
10.
De Scheerder IK Wang K Zhou XR Verbeken E Keelan MH Horn JB Sahota H Kipshidze N 《The Journal of invasive cardiology》1998,10(5):263-268
BACKGROUND: It is believed that restenosis following coronary interventions is the result of endothelial denudation that leads to thrombus formation, vascular remodeling, and smooth muscle cell proliferation. We previously demonstrated that low power red laser light (LPRLL) irradiation enhances endothelial cell growth in vitro and in vivo and reduces restenosis in a small animal model. The present study investigated the effectiveness of intravascular LPRLL therapy in the reduction of restenosis following stenting in a porcine model. METHODS AND RESULTS: Stents were placed in the right coronary artery of domestic cross-bred pigs. After stent deployment, an additional inflation was performed with the laser-balloon. In group I (n = 18) no LPRLL was used; group II (n = 10) received LPRLL dosage of 10 mW for 1 minute; group III (n = 10) received LPRLL dosage of 34 mW for 1 minute. Quantitative coronary analysis of the stented vessel was performed before, immediately after stenting, and at 6 weeks. The pigs were sacrificed and histologic and planimetric analysis conducted. At 6 weeks, minimal luminal stent diameter was significantly narrower in the control group compared to the higher dose group (p < 0.05), late loss correlated inversely proportional to the dose used (r = 0.9; p < 0.03), these results were confirmed by morphometric analysis. Neointimal area was also significantly decreased in the higher dose group. CONCLUSIONS: Intravascular LPRLL contributes to reduction of angiographic restenosis and hyperplastic reaction in this animal model and seems to be dose dependent. 相似文献