Unowocześnienie diagnostyki nocnej napadowej hemoglobinurii – wstępne wyniki badań przy użyciu odczynnika FLAER

Blood samples of 50 patients diagnosed for paroxysmal nocturnal haemoglobinuria (PNH) were examined using FLAER screening test for the detection of PNH clone. The results were compared with routine testing using monoclonal antibodies detecting CD59 and CD66b antigens on neutrophils and CD59 on erythrocytes. Assay sensitivity (≥ 0.1%) and PNH clone size analysed using FLAER and anti-CD66b antibodies was fully consistent (R² = 0.9991) and higher than with anti-CD59 antibodies. Preliminary results indicate that for diagnosis of PNH the FLAER based test is quick, reliable and cost-effective. It might seem that the conventional screening methods could therefore be abandoned. It was found however, that in some cases of abnormal granulocyte population, additional studies should be performed using multiparameter flow cytometry blood cell analysis.     

Infektionsitätsänderungen und Aktivierungsprozesse beim Virus der Maul- und Klauenseuche

Ohne Zusammenfassung     

Pure compound libraries; a new perspective for natural product based drug discovery

What often distinguishes the leaders in drug discovery and development from the rest is the quality of their compound libraries and the ease of access that they have to the information within those libraries. The screening of natural products can provide greater structural diversity than standard synthetic chemistry and offers significant opportunities for finding novel low molecular weight lead compounds. However, which strategy is the best for natural-product-based drug discovery? Two well established but relatively time consuming approaches are the screening of crude extracts and pre-fractionated extracts. This case study describes a third, pure-compound-screening approach, and discusses its benefits and pitfalls.     

Data on the biological properties of olefin sulfate

In the experiments on hybrid mice CBA X C57Bl6 the effect of the preparation Progress with different (16.5% and 24.5%) content of the surface-active agent--olefine sulphate on benz(a)pyrene induced carcinogenesis has been studied. The cutaneous application of the substance has evidenced enhanced carcinogenesis under the exposure to a larger of the two dosages: skin tumors in the experimental group were noted in 68.75% of animals, in the control group (application of the carcinogen)--in 19.44%. Under the condition of peroral administration of the agent no enhancement of carcinogenesis was revealed, meanwhile proventricular tumors appeared in the group where the carcinogen was administered without the preliminary carcinogen exposure.