全文获取类型
收费全文 | 426篇 |
免费 | 37篇 |
国内免费 | 2篇 |
专业分类
儿科学 | 38篇 |
妇产科学 | 1篇 |
基础医学 | 92篇 |
口腔科学 | 12篇 |
临床医学 | 30篇 |
内科学 | 85篇 |
皮肤病学 | 5篇 |
神经病学 | 4篇 |
特种医学 | 52篇 |
外科学 | 21篇 |
综合类 | 8篇 |
预防医学 | 13篇 |
眼科学 | 57篇 |
药学 | 19篇 |
肿瘤学 | 28篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 4篇 |
2018年 | 9篇 |
2017年 | 7篇 |
2016年 | 8篇 |
2015年 | 13篇 |
2014年 | 20篇 |
2013年 | 13篇 |
2012年 | 8篇 |
2011年 | 6篇 |
2010年 | 11篇 |
2009年 | 21篇 |
2008年 | 15篇 |
2007年 | 5篇 |
2006年 | 15篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 12篇 |
2002年 | 10篇 |
2001年 | 18篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 21篇 |
1997年 | 32篇 |
1996年 | 28篇 |
1995年 | 16篇 |
1994年 | 26篇 |
1993年 | 14篇 |
1992年 | 7篇 |
1991年 | 11篇 |
1990年 | 4篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 7篇 |
1979年 | 1篇 |
1976年 | 5篇 |
1975年 | 1篇 |
1972年 | 2篇 |
1970年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有465条查询结果,搜索用时 15 毫秒
1.
Benign intracranial hypertension and recombinant growth hormone therapy in Australia and New Zealand
PA Crock JD McKenzie AM Nicoll NJ Howard W Cutfield LK Shield G Byrne 《Acta paediatrica (Oslo, Norway : 1992)》1998,87(4):381-386
Benign intracranial hypertension (BIH) is reported in three children from Australia and one from New Zealand, who were being treated with recombinant human growth hormone (rhGH). Three males and one female, aged between 10.5 and 14.2 y, developed intracranial hypertension within 2 weeks to 3 months of starting treatment. A national database, OZGROW, has been prospectively collecting data on all 3332 children treated with rhGH in Australia and New Zealand from January 1986 to 1996. The incidence of BIH in children treated with growth hormone (GH) is small, 1.2 per 1000 cases overall, but appears to be greater with biochemical GHD (<10IUml -1 ), i.e. 6.5/1000 (3 in 465 cases), relative risk 18.4, 95% confidence interval 1.9-176.1, than in all other children on the database. The incidence in patients with Turner's syndrome was 2.3/1000 (1 in 428 cases). No cases in patients with partial GHD (10–20 IUml -1 ) or chronic renal failure were identified. Possible causative mechanisms are discussed. The authors'practice is now to start GH replacement at less than the usual recommended dose of 14IUm-2 week-1 in those children considered to be at high risk of developing BIH. Ophthalmological evaluation is recommended for children before and during the first few months following commencement of rhGH therapy and is mandatory in the event of peripheral or facial oedema, persistent headaches, vomiting or visual symptoms. The absence of papilledema does not exclude the diagnosis. 相似文献
2.
I. N. Nifontova N. V. Sats V. L. Surin D. A. Svinareva M. E. Gasparian N. J. Drize 《Bulletin of experimental biology and medicine》2008,145(1):133-136
We developed a method for gene transfer into mesenchymal stromal cells. Lentivirus vector containing green fluorescent protein
gene for labeling stromal and hemopoietic precursor cells was obtained using two plasmid sets from different sources. The
vector was injected into the femur of mice in vivo and added into culture medium for in vitro infection of the stromal sublayer of long-term bone marrow culture. From 25 to 80% hemopoietic stem cells forming colonies
in the spleen were infected with lentivirus vector in vivo and in vitro. Fibroblast colony-forming cells from the femoral bones of mice injected with the lentivirus vector carried no marker gene.
The marker gene was detected in differentiated descendants from mesenchymal stem cells (bone cavity cells from the focus of
ectopic hemopoiesis formed after implantation of the femoral bone marrow cylinder infected with lentivirus vector under the
renal capsule of syngeneic recipient). In in vitro experiments, the marker gene was detected in sublayers of long-term bone marrow cultures infected after preliminary 28-week
culturing, when hemopoiesis was completely exhausted. The efficiency of infection of stromal precursor cells depended on the
source of lentivirus. The possibility of transfering the target gene into hemopoietic precursor cells in vivo is demonstrated. Stromal precursor cells can incorporate the provirus in vivo and in vitro, but conditions and infection system for effective infection should be thoroughly selected.
__________
Translated from Kletochnye Tehnologii v Biologii i Meditsine, No. 1, pp. 25–28, January, 2007 相似文献
3.
4.
5.
Using a newly devised 50-channel photometer which records the opacity of growing bacterial cultures, it was shown that the time taken by cultures diluted 1/1000 in fresh broth to reach 50% of the opacity of a fully grown culture was inversely related to the concentration of organisms in the original culture. This relation was used to determine the numbers of survivors after exposure to benzylpenicillin and gentamicin alone and in combination. The procedure is commended as a labour-saving and potentially rapid method of obtaining comprehensive information on the bactericidal action and interaction of antibiotics. 相似文献
6.
I. N. Nifontova D. A. Svinareva I. L. Chertkov N. I. Drize V. G. Savchenko 《Bulletin of experimental biology and medicine》2008,145(5):629-633
We studied the effects of chronic administration of granulocyte colony-stimulating factor in nonmobilizing doses to mice.
Over 18 months of the study, 55% animals of the treatment group died of unknown cause, blood diseases and tumors were found
in 20% mice, and in 5% animals pathological changes were absent. Control mice had no diseases (normal values of total and
differential leukocyte count). The diagnoses made over the first 7 months mainly included myeloproliferative diseases. Solid
tumors were found at later terms. Suppurative inflammation at the site of injection was observed in all mice after 3-month
treatment with granulocyte colony-stimulating factor. Our results indicate that chronic administration of granulocyte colony-stimulating
factor in low doses leads to the development of etiologically different tumors and sharply reduced animal life span. The use
of granulocyte colony-stimulating factor during allogeneic transplantation of hemopoietic stem cells can be hazardous for
donors.
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 145, No. 5, pp. 568–573, May, 2008 相似文献
7.
KM Kanal NJ Hangiandreou AM Sykes HE Eklund PA Araoz JA Leon BJ Erickson 《Journal of digital imaging》2002,14(1):30-37
The aims of this work were to measure the accuracy of one continuous speech recognition product and dependence on the speaker's
gender and status as a native or nonnative English speaker, and evaluate the product's potential for routine use in transcribing
radiology reports. IBM MedSpeak/Radiology software, version 1.1 was evaluated by 6 speakers. Two were nonnative English speakers,
and 3 were men. Each speaker dictated a set of 12 reports. The reports included neurologic and body imaging examinations performed
with 6 different modalities. The dictated and original report texts were compared, and error rates for overall, significant,
and subtle significant errors were computed. Error rate dependence on modality, native English speaker status, and gender
were evaluated by performing ttests. The overall error rate was 10.3 +/- 3.3%. No difference in accuracy between men and women
was found; however, significant differences were seen for overall and significant errors when comparing native and nonnative
English speakers (P = .009 and P = .008, respectively). The speech recognition software is approximately 90% accurate, and
while practical implementation issues (rather than accuracy) currently limit routine use of this product throughout a radiology
practice, application in niche areas such as the emergency room currently is being pursued. This methodology provides a convenient
way to compare the initial accuracy of different speech recognition products, and changes in accuracy over time, in a detailed
and sensitive manner. 相似文献
8.
9.
This study was designed to compare the growth of Pakistani schoolchildren in the UK with the 1990 UK growth standards. Measurements of height, weight, and sitting height were performed on 785 Pakistani schoolchildren aged 5-14 years with the mean values for each age and sex being plotted on the UK growth standards. The results were expressed as SD scores relative to the 1990 reference data. The mean height for the boys was only 0.2 SD scores below the mean for the new growth standards with the mean height for the girls being 0.4 SD scores below the mean. The mean values for weight and body mass index were 0.3 and 0.5 SD scores less than the mean for boys and girls respectively. This study demonstrates that the growth of Pakistani schoolchildren in the UK is comparable to the 1990 UK growth standards with only minor differences. It is not safe to assume that short stature or low body weight in a Pakistani child is due to his or her ethnic background. 相似文献
10.
Comprehensive mutational scanning of the p53 coding region by two- dimensional gene scanning 总被引:2,自引:0,他引:2
A comprehensive mutational scanning test for the p53 coding region based on
multiplex PCR and two-dimensional DNA electrophoresis was designed and
evaluated. In a 2-step multiplex PCR, the p53 coding region (exons 2-11)
was amplified as a single 8646-bp fragment by long- distance PCR in step
one. This fragment served as a template for the subsequent co-amplification
of the individual exons in two multiplex groups in step two. The multiplex
products were then separated, first on the basis of size in non-denaturant
polyacrylamide gels and then on the basis of sequence by denaturing
gradient gel electrophoresis (DGGE). Primers for optimal PCR, melting
behavior and 2-D gel distribution were designed using a recently developed
computer program. The resulting two-dimensional gene scanning (TDGS) test
was evaluated by screening, in a blinded fashion, 29 coded DNA samples from
Li- Fraumeni syndrome patients with previously identified germline
mutations. All mutations were correctly detected. This assay provides an
accurate, cost-effective and non-radioactive method for simultaneous
mutational scanning of all p53 coding exons.
相似文献