硫酸多糖对体外人脐静脉内皮细胞损伤的保护作用

研究表明,硫酸多糖体外对多聚阳离子和氧自由基损伤的人脐静脉内皮细胞有保护作用。肝素、硫酸软骨素A抗多聚阳离子损伤作用比同浓度低分子肝素和甘糖酯强。肝素、硫酸软骨素A、甘糖酯抗氧自由基损伤作用优于同浓度低分子肝素。结果显示硫酸多糖有保护血管内皮的作用,其作用可能与所带阴离子基团有关。     

Gastric duplication in an adult mimicking mucinous cystadenoma of the pancreas

Gastric duplication cyst (GDC) in an adult can have several clinical presentations. A review of the literature showed previously reported cases of GDC presenting as pancreatic pseudocyst or with greatly raised concentrations of carbohydrate antigen 19-9 (CA 19-9). It is often difficult to discriminate GDC from pancreatic cystic tumour, in particular pancreatic mucinous cystadenoma, in which concentrations of carcinoembryonic antigen and CA 19-9 are classically raised. This report describes an adult case of GDC mimicking a mucinous cystadenoma of the pancreas. This is the first report of a simultaneous increase in carcinoembryonic antigen and CA 19-9 in GDC in the absence of malignancy. Although few cases of carcinoma arising from a GDC having been reported, the production of oncofetal antigens raises the problem of a precancerous condition in long standing intestinal duplications. In this situation surgical resection must be performed.     

p16INK4A and p15INK4B gene deletions in primary leukemias

The 9p21 locus has been deleted at a high frequency in a wide variety of tumors. Recently, two genes, p16INK4A and p15INK4B (also called MTS1 and MTS2), have been localized in close proximity at the 9p21 locus, encoding cyclin-dependent kinases 4/6 inhibitors of relative molecular mass 16 kD and 15 kD, respectively and also found to be deleted at a high frequency in tumor cell lines. We analyzed p16INK4A and p15INK4B genes in 178 cases of primary leukemias including 81 cases of chronic lymphocytic leukemia (CLL), seven of hairy cell leukemia (HCL), seven of chronic myelogenous leukemia (CML), 43 of acute myelogenous leukemia (AML), 27 of acute lymphoblastic leukemia (ALL), and 13 of myelodysplastic syndrome (MDS) by Southern blot analyses. The ALL cases showed a relatively high frequency of homozygous deletions (22%, 6 of 27) at the p16INK4A gene locus. Interestingly, of the six cases with p16INK4A homozygous deletions, only three showed homozygous deletions at the p15INK4B gene. In 81 CLL patients, we detected one homozygous and five heterozygous deletions at both the p16INK4A and p15INK4B genes and two heterozygous deletions at the p16INK4A gene alone. Deletion of these two genes in AML cases is relatively low (9%). We did not detect deletions in any of the MDS, HCL, and CML cases examined. Sequence analyses of p16INK4A gene of six CLL cases with heterozygous deletion at this locus showed a 27-bp deletion at the splice acceptor site of intron 1 in one case and changes in the coding sequence in three other cases. The data presented in this report showed that (1) p16INK4A and p15INK4B genes are preferentially deleted homozygously in ALL and heterozygously in CLL cases with frequent mutation in the second allele, and (2) p16INK4A gene appears to be more frequently deleted than p15INK4B gene.     

难治性青光眼两种治疗方法的比较

临床资料 2003-02／2004—10，难治性青光眼36例36眼，(男19，女17)例；年龄37—81(平均59．6)岁；术前视力：无光感6例，光感15例，手动13例，眼前数指2例；术前眼压：在5．59—10．77(平均8．01)kPa；1例系玻切术后继发性青光眼，35例系新生血管性青光眼(其中：视网膜静脉阻塞15例，糖尿病视网膜病变18例，原因不明2例)．①眼内窥镜下睫状体光凝术18例：在角膜缘环形剪开结膜，充分止血；在     

Effects of PEEP on oxygenation and respiratory mechanics during one-lung ventilation

Background. One-lung ventilation-related hypoxaemia (OLV-RH)can occur in patients with healthy lungs. In this case, PEEPfrequently improves oxygenation. The aim of this study was todetermine, in a healthy lung model of OLV, whether the increasein PEEP improved oxygenation and whether the mechanisms involvedinclude both inspiratory lung recruitment and an end-expiratorylung volume increase. Since inhaled nitric oxide (iNO) may havea synergistic effect on oxygenation in the case of PEEP-inducedrecruitment, their association was also tested. Methods. Twenty pigs were studied during open-chest, left OLV.Arterial blood gases and haemodynamic variables were measuredat different levels of PEEP (0, 5, 10 and 15 cm H₂O) appliedin random order with or without iNO 4 p.p.m. Pressure–volumecurves were measured at each level of PEEP. Results. PEEP₅ and PEEP₁₀ improved / ratio (P<0.005) and shunt (P<0.005) regardlessof the presence of iNO. PEEP₁₅ improved oxygenation and shuntonly when it was associated with iNO (P<0.001). Whereas PEEP₅,PEEP₁₀ and PEEP₁₅ were associated with a significant increasein end-expiratory volume (P<0.001), only PEEP₅ and PEEP₁₀were associated with continuous lung volume recruitment (P<0.01).Moreover, PEEP₁₅ induced a significant decrease in linear compliance(P<0.001). Conclusions. In a healthy porcine lung model of OLV-RH, moderatePEEP can improve oxygenation. This effect implies both expiratoryand inspiratory pulmonary recruitment. Co-administration of4 p.p.m. iNO was ineffective.     

毒物分析用于1例苯巴比妥中毒患者抢救

目的：为临床提供大剂量苯巴比妥中毒患者血药浓度与临床症状的关系。方法;介绍一例进行中毒药物测定的患者,报告并解释其抢救过程血药浓度与相应的临床症状。结果：中毒患者在血药浓度指导下,成功地采用血液灌流进行抢救。结论：毒物分析能有效指导临床苯巴比妥中毒患者抢救,血药浓度与临床症状密切相关。