全文获取类型
收费全文 | 418篇 |
免费 | 20篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 3篇 |
妇产科学 | 4篇 |
基础医学 | 89篇 |
口腔科学 | 17篇 |
临床医学 | 23篇 |
内科学 | 74篇 |
皮肤病学 | 8篇 |
神经病学 | 24篇 |
特种医学 | 6篇 |
外科学 | 120篇 |
预防医学 | 19篇 |
眼科学 | 6篇 |
药学 | 23篇 |
中国医学 | 2篇 |
肿瘤学 | 22篇 |
出版年
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 21篇 |
2020年 | 9篇 |
2019年 | 13篇 |
2018年 | 17篇 |
2017年 | 12篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 16篇 |
2013年 | 18篇 |
2012年 | 37篇 |
2011年 | 31篇 |
2010年 | 17篇 |
2009年 | 12篇 |
2008年 | 17篇 |
2007年 | 18篇 |
2006年 | 15篇 |
2005年 | 19篇 |
2004年 | 31篇 |
2003年 | 20篇 |
2002年 | 16篇 |
2001年 | 18篇 |
2000年 | 10篇 |
1999年 | 19篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有442条查询结果,搜索用时 15 毫秒
1.
2.
C. Wood B. Sina C. G. Webster D. Kurgansky C. B. Drachenberg E. A. Reedy 《Journal of cutaneous pathology》1992,19(2):128-133
A 57-year-old woman with cutaneous mastocytosis of 23 years duration developed a hyperpigmented abdominal plaque composed of confluent indurated papules that enlarged for a period of 1 year to 12 x 8 cm. Biopsy showed dermal infiltration by closely packed spindle-shaped mast cells, fibroblasts, collagen, and scattered lymphocytes, predominantly T-suppressor cells. Electron microscopy showed close contact between mast cells, fibroblasts, and lymphocytes. Piecemeal mast cell degranulation and extrusion of mast cell granules was seen, with rare mast cell granules in fibroblasts, and collagen fibers in peripheral and perinuclear endoplasmic reticulum of mast cells. the term Fibrous mastocytoma is suggested for this tumor-like dermal fibrosis, possibly induced by lymphokines. 相似文献
3.
4.
5.
Cinthia Castro do Nascimento Odair Aguiar Junior Vania D’Almeida 《International journal of clinical and experimental pathology》2014,7(6):3488-3497
Mucopolysaccharidosis (MPS) I is a lysosomal storage disorder (LSD) that is characterised by alpha-L-iduronidase (Idua) deficiency and continuous deposition of glycosaminoglycans (GAGs), which consequently interferes with cell signalling mechanisms and results in multisystemic and progressive symptoms. The animal model of MPS I (Idua-/-) has been widely studied to elucidate the consequences and progression of the disorder; however, studies specifically assessing the male reproductive tract are lacking. The aim of this study was to evaluate some of the reproductive characteristics of male MPS I mice in two phases of life. Reproductive organ biometry, sperm counts, sperm morphological evaluation, plasma testosterone measurements and histopathological, histomorphometrical and immunohistochemical analysis were performed in 3- and 6-month-old C57BL/6 Idua+/+ and Idua-/- mice. Seminal vesicle weights were decreased in both the 3- and 6-month-old Idua-/- mice. Decrease in sperm counts and the majority of the histopathological signs were observed in the 6-month-old Idua-/- mice. No differences were detected in the sperm morphological analysis. Immunohistochemistry revealed that seminiferous tubules from 3-month-old Idua-/- mice were more intensely stained with anti-caspase-3 than 3-month-old Idua+/+ mice, but no difference was found at 6 months. These results suggest that MPS I interferes with male reproductive parameters both in 3 and 6-month-old animals and histopathological signs are more pronounced in 6-month-old mice, indicating that the effects of the disorder may intensify with the disease progression. 相似文献
6.
7.
Danieli B. Martins Cinthia M. Mazzanti Roselia Spanevello Roberta Schmatz Juliana F. Cargnelutti Candice Schmidt Carolina K. Traesel Naiara Stefanello Vera M. Morsh Maria R. C. Schetinger Sonia T. A. Lopes 《Comparative clinical pathology》2012,21(3):265-268
Health sciences have recently discovered the medical uses of nandrolone decanoate (ND), an androgenic anabolic steroid (AAS), and reported its use in human and animal patients. Clinical evidences suggest that the AAS excess may affect the cholinergic system, which is responsible for several vital functions like learning, memory, and the organization of the movements. Thus, our aim is to research the subchronic effect of ND when administered in varying doses on the acetylcholinesterase (AChE) activity in these brain structures: cerebellum (CE), hippocampus, striatum (ST), and cortex of adult rats. We used 36 male Wistar rats, which were divided into six groups (n?=?6). The groups were divided into: G1—control (physiologic solution), G2—diluents control (only an oleaginous vehicle of vegetal origin—olive oil), G3—0.42?mg?kg?1 of ND, G4—1.8?mg?kg?1 of ND, G5—4.6?mg?kg?1 of ND, and G6—10.0?mg?kg?1 of ND. We applied the doses once every week during a 3-week period. The values obtained demonstrated a significant increase in the AChE activity (referring to ST and CE for the 4.6 and 10.0?mg?kg?1 doses of ND). The ND causes increase in AChE activity, which could impair neurotransmission and cholinergic modulation. 相似文献
8.
9.
Andrea Sánchez Paulina Bustos Paula Honorato Carlos F. Burgos Natalia Barriga Cinthia E. Jannes Katia Sáez Rodrigo Alonso Sylvia Asenjo Claudia Radojkovic 《Journal of clinical lipidology》2021,15(2):366-374.e1
BackgroundFamilial hypercholesterolemia (FH) is an inherited disorder mainly caused by mutations in the LDL receptor (LDL-R) and characterized by elevation of low-density lipoprotein cholesterol (LDL-C) levels and premature cardiovascular disease.ObjectiveIn this study, we evaluated the clinical phenotype of the p.Asp47Asn, described as an uncertain pathogenic variant, and its effect on the structure of LDL-R and ligand interactions with apolipoproteins.Methods27 children and adolescents with suspected FH diagnosis were recruited from a pediatric endocrinology outpatient clinic. Blood samples were collected after 12 h fasting for lipid profile analysis. DNA sequencing was performed for six FH-related genes by Ion Torrent PGM platform and copy number variation by MLPA. For index cases, a familial cascade screening was done restricted to the same mutation found in the index case. In silico analysis were developed to evaluate the binding capacity of LDL-R to apolipoproteins B100 and E.ResultsLipid profile in children and adolescents demonstrated higher LDL-C levels in p.Asp47Asn carriers compared to the wild type genotype. In silico analysis predicted a reduction in the binding capacity of the ligand-binding modules LA1-2 of p.Asp47Asn LDL-R for ApoB100 and ApoE, which was not produced by local structural changes or folding defects but as a consequence of a decreased apparent affinity for both apolipoproteins.ConclusionThe clinical phenotype and the structural effects of p.Asp47Asn LDL-R mutation suggest that this variant associates to FH. 相似文献