Pten基因敲除对过氧化物酶家族表达和活性氧水平的影响

目的:探讨Pten基因敲除后对过氧化物酶家族(Peroxiredoxins,Prdxs)水平和活性氧水平的影响.方法:采用Western印迹和化学/荧光发光分析法分别检测了在Pten / MEF和Pten-/-MEF细胞中PRDXs的表达和细胞内活性氧水平.结果:Western印迹结果显示,与Pten / MEF细胞相比,Pten-/-MEF细胞PRDX Ⅰ,Ⅱ,Ⅴ,Ⅵ蛋白水平下调,PRDX Ⅲ不变,PRDX Ⅳ上调.DCFH探针标记后流式结果显示Pten-/-MEF 细胞活性氧荧光值显著高于对照Pten / MEF细胞(P＜0.05).结论:Pten基因敲除引起数种PRDXs表达下调,细胞内活性氧水平增高.     

冬凌草甲素通过改变Bax/Bcl-xL表达激活caspase-3诱导A375-S2细胞凋亡

目的　研究冬凌草甲素诱导人黑色素瘤A375 S2细胞凋亡的作用原理。方法　形态学观察 ,DNA凝胶电泳法及WesternBlot法。结果　冬凌草甲素能明显诱导A375 S2细胞发生凋亡 ,其作用呈明显的量效关系和时间依赖性。形态学观察可见凋亡小体的形成 ,琼脂糖凝胶电泳可见凋亡典型的DNA梯带 ;caspase 3的抑制剂能阻止caspase 3的活力升高 ;免疫印迹结果显示冬凌草甲素作用A375 S2细胞 12h改变Bax与Bcl xL蛋白的表达 ;且发现caspase 3的底物PARP蛋白在 12h时被降解。结论　冬凌草甲素 (34 3μmol·L-1)诱导A375 S2细胞凋亡 ,这种作用是通过改变了Bax/Bcl xL的表达比率 ,激活caspase 3而实现的。     

Rapid analysis of piperazine ferulate tablets by optic-fiber sensing technology and the similarity of ultraviolet spectra

A rapid analysis method of piperazine ferulate tablets by optic-fiber sensing technology with UV–vis absorption spectrum was established. Qualitative and quantitative data were obtained and compared by maximum and minimum wavelength, absorbance and contrast spectra. Similarity method was used to identify authenticity of drugs. The difference of contents measured by this method and UV determination method in China Pharmacopoeia showed no statistical significance (P>0.05), while the similarity can be used as a parameter to identify the authenticity of drugs.     

综合干预联合网络咨询对社区精神分裂症患者预后影响的1年随访

目的:探讨社区精神分裂症患者综合干预中联用网络咨询1年的效果。方法:对门诊精神分裂症患者106例进行可变剂量抗精神病药治疗12个月,分为实地随访干预结合网络咨询(试验组)25例和实地随访干预(对照组)81例。入组时以阳性与阴性症状量表(PANSS)、社会功能量表较基线的差值作为主要疗效指标;以网络咨询相关问题调查网络咨询的可接受性。结果:PANSS量表分随治疗下降,1个月后评分下降(P0.01),保持降低趋势至12个月,治疗前组间差异无统计学意义。试验组社会功能损害渐改善(P0.05),对照组改善不明显,12个月组间差异有统计学意义(P0.05)。进行网络咨询相关问题调查表调查,试验组(72.0%)用网络解决医学问题者明显多于对照组(40.0%),差异有统计学意义(χ2=6.89,P0.01)。多数患者(70.0%)看好网络咨询前景等,但超过60.0%认为收费对其有影响,建议的网络咨询价格明显低于面对面咨询(P0.001)。结论:社区精神分裂症综合干预中联用网络咨询有利于减轻症状和改善社会功能,网络咨询有利于方便服务,患者愿接受网络咨询。     

Spatial and temporal EEG dynamics of motion sickness

This study investigates motion-sickness-related brain responses using a VR-based driving simulator on a motion platform with six degrees of freedom, which provides both visual and vestibular stimulations to induce motion sickness in a manner that is close to that in daily life. Subjects' brain dynamics associated with motion sickness were measured using a 32-channel EEG system. Their degree of motion sickness was simultaneously and continuously reported using an onsite joystick, providing non-stop behavioral references to the recorded EEG changes. The acquired EEG signals were parsed by independent component analysis (ICA) into maximally independent processes. The decomposition enables the brain dynamics that are induced by the motion of the platform and motion sickness to be disassociated. Five MS-related brain processes with equivalent dipoles located in the left motor, the parietal, the right motor, the occipital and the occipital midline areas were consistently identified across all subjects. The parietal and motor components exhibited significant alpha power suppression in response to vestibular stimuli, while the occipital components exhibited MS-related power augmentation in mainly theta and delta bands; the occipital midline components exhibited a broadband power increase. Further, time series cross-correlation analysis was employed to evaluate relationships between the spectral changes associated with different brain processes and the degree of motion sickness. According to our results, it is suggested both visual and vestibular stimulations should be used to induce motion sickness in brain dynamic studies.     

肿瘤晚期患者癌性疼痛的家庭综合干预治疗

目的探讨家庭综合干预治疗对肿瘤晚期患者癌性疼痛的疗效及安全性。方法选取200例经病理确诊的肿瘤晚期患者,随机分为试验组和对照组各100例。试验组按照世界卫生组织(WHO)三阶梯止痛原则,给予家庭镇痛治疗,并进行家庭护理干预。对照组患者按照其自主择医镇痛的意愿,不增加干预。分别于初诊和治疗后4个月对两组患者进行疼痛强度、生活质量、心理功能以及医疗费用的评估并进行比较。结果试验组患者药物镇痛有效率为93%,对照组为78%,两者差异有统计学意义(P<0.01)。试验组生活质量、心理功能等方面有明显提高和改善(P<0.01),且优于对照组(P<0.01),同时总体医疗费用也低于对照组(P<0.05)。结论对肿瘤晚期患者癌性疼痛进行家庭综合干预治疗安全可靠,切实可行,镇痛效果好,值得推广。     

角膜移植患者外周血中T细胞CD28分子的表达及其意义

目的　探讨角膜移植患者外周血T细胞及亚群CD2 8分子表达的变化及其意义。方法　于术前1d、术后第1、2、3、4周,应用流式细胞仪检测2 5例角膜移植患者外周血中CD2 8分子表达水平的变化。结果　角膜高血管化植床组患者术后外周血T细胞CD2 8分子表达比角膜无血管化植床组患者明显增高,也比术前明显增高(P <0 .0 1) ;6例发生排斥反应的患者均出于高血管化植床组;术后早期外周血T细胞中以CD4 + 细胞为主。结论　外周血T细胞CD2 8分子表达与角膜移植排斥反应关系密切,检测角膜移植患者外周血中的CD2 8分子表达可更早监测免疫排斥反应的发生     

Oridonin induces apoptosis of HeLa cells via altering expression of Bcl-2 ／ Bax and activating caspase-3 ／ ICAD pathway

INTRODUCTIONHerbal medicine, Donglingcao (rabdosiarubescens), has been traditionally used in China for thetreatment of various diseases such as leukemia.Oridonin (Fig 1) is a diterpenoid compound isolated fromRabdosia rubescens (hemsl). It has various pharmaco-logical and physiological effects such as anti-inflammation, anti-bacteria, anti-tumor[1-3] and has beenused for the treatment of human cancers, especiallyFig 1. Chemical structure of oridonin.esophageal carcinoma[4]. This comp…