Anti-platelet fraction from Galega officinalis L. inhibits platelet aggregation

A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The fraction inhibits platelet aggregation initiated by 25 microM adenosine 5'-diphosphate (ADP), 100 microg/ml collagen, and 0.8 U/ml thrombin with the 50% inhibiting concentration (IC(50)) being 11.2 microg/ml for ADP, and the IC(100) being 15.1 microg/ml for collagen and IC(100) 19.6 microg/ml for thrombin.     

Distribution and characterization of the melatonin receptors in the hypothalamus and pituitary gland of three domestic ungulates

Abstract: With some exceptions, in most of the mammals the pituitary pars tuber-alis and the hypothalamic suprachiasmatic nuclei are reportedly the main targets for the pineal hormone melatonin. However, it is not known if the conspicuous diversity in the distribution pattern of melatonin binding sites in these areas depicts differences in reproductive behavior observed in the seasonally breeding species in the temperate zones. We explored the distribution and the characteristics of melatonin binding sites in the hypothalamus and pituitary of three species (bovine, horse, and donkey) different in terms of seasonal reproductive competence. The topographical localization, investigated by in vitro autoradiography, revealed 2-[¹²⁵I]iodomelatonin binding sites only in the pituitary gland in all three species, primarily in the pars tuberalis (PT), but also in the pars distalis (PD) and pars intermedia (PI). Kinetic, inhibition, and saturation studies, performed by means of in vitro binding, revealed presence of a single class high affinity binding sites. The K_d values, melatonin, and 2-iodomelatonin K_j values were in the low picomo-lar range. Coincubation with GTP7S inhibited 2-[¹²⁵ I]iodomelatonin binding, demonstrating that these putative receptors are linked to a G protein in their signal-transduction pathway. The hypothalamus was devoid of specific binding. In conclusion, the results suggest that in these species, the hypophysis may be a principal target for the melatonin action on the reproductive system.     

Regulation of the androgen receptors in the Harderian gland of the male Syrian hamster: Influence of photoperiod, castration, and chronic melatonin treatment

Male Syrian hamsters that were exposed for 8 weeks to short photoperiod (LD 10:14) or treated with melatonin in the late afternoon under long photoperiod conditions (LD 14:10) had a significantly higher content of androgen receptors in the Lipidex-purified soluble fractions isolated from the Harderian glands as compared to the long photoperiod (LD 14:10) exposed controls. Simultaneous computer-assisted analyses of all series of saturation and competition experiments revealed that the numerical value of the apparent Kd, as determined by using the synthetic androgen R-1881 (methyltrienolone), was not different between the experimental groups, and ranged from 0.050 to 0.067 nM. Of the principal natural androgens, testosterone (T) was most potent in inhibiting methyltrienolone binding to the receptor (Ki values from 0.33 to 0.55 nM), and 5 alpha-dihydrotestosterone (DHT) and delta 4-androstenedione (AD) were less effective (Ki values between 1 and 1.9 nM). In the hypothalami and pituitaries of the same animals, used in parallel control assays, DHT was twice as potent as T. Short-term castration (24 hr post-orchidectomy) did not result in significant changes in the receptor binding characteristics. Following 8 weeks exposure to a long photoperiod (LD 14:10) the Bmax values demonstrated a four-fold increase in castrated animals (179 fmoles/mg protein vs. 47 fmoles/mg protein) over intact controls. The relative binding affinity of the major androgens under these conditions remained unchanged, with the exception of AD, where a five-fold increase in the numerical Ki values (decrease in the binding affinity) was recorded (Ki = 9.6 nM).(ABSTRACT TRUNCATED AT 250 WORDS)     

Case report: Percutaneous balloon pericardiotomy using Inoue balloon for patients with massive pericardial effusion

Two patients presenting with huge pericardial effusion were treated with pericardiotomy, using an Inoue balloon dilating catheter, to prevent recurrences and achieve symptoms relief. The procedure is a modification of an original technique reported by Palacios et al. gaining advantage of the Inoue balloon self positioning abilities and low profile. No early recurrence was observed. In both cases the effect was excellent and lead to quick ambulation sparing the patients the risk of more aggressive surgical procedure and general anesthesia. It is concluded that percutaneous balloon pericardiotomy supported by the Inoue balloon unique versatility is a simple and safe way to drain massive pericardial effusions.     

Cutting the encircling band

PURPOSE: To report the effect of cutting the encircling band on ocular blood flow. METHODS: In an interventional case series of 27 eyes with an encircling band, the ocular pulse amplitude (OPA) was measured and pulsatile ocular blood flow (POBF) derived and compared with fellow eyes. The bands were cut in 11 eyes and the POBF measured at 1 month, 3 months, and 1 year. Changes in POBF, buckle height, and visual acuity were determined. RESULTS: The presence of the encircling band reduced the POBF to a mean of 43% (P < 0.001) of the fellow eye. Cutting the band resulted in a mean recovery to 85.6%. No retina detached after cutting, buckle height was only slightly diminished, and the visual acuity was unaffected. CONCLUSION: An encircling band diminishes the POBF. Cutting the band restores significant flow.     

Distribution and characterization of melatonin receptors in the brain of the Japanese quail, Coturnix japonica

2-[¹²⁵I]iodomelatonin was used to study the distribution and properties of the melatonin receptor in the Japanese quail brain. High receptor density was detected in the major targets of direct retinal input (optic tectum, nucleus of the optic basal rout, ventrolateral geniculate nucleus), as well as areas representing terminals in the visual pathways (nucleus rotundus, ectostriatum, thalamo-hyperstriatal pathway). Binding was also found in the piriform cortex, the hypophyseal pars tuberalis, the oculomotorius nucleus and the associated Edinger-Westphal nucleus, and in the nuclei of the third, fourth and sixth cranial nerves. A comparison of the receptor pharmacological profile to that of the mammalian brain demonstrated pharmacological identity of the two binding sites. In the saturation experiments, GPT_γS decreased the binding affinity, numerical K_d values increasing from ≈ 35 pM to ≈ 150 pM.     

Computerized cognitive examination of the elderly (ECO): The development of a neuropsychological examination for clinic and population use

The development of a computerized neuropsychological examination (ECO), designed to evaluate intellectual functioning in elderly populations, is described. The examination is based on clinical and laboratory observations of cognitive functioning in normal and pathological ageing. ECO provides extensive information on attention, memory, and visuospatial and linguistic functioning, and the use of a touch screen permits the recording of reaction time and response delays. Information regarding qualitative aspects of responses is also recorded. The examination takes relatively little time given the extent of the information obtained (approximately 40 min), is highly acceptable to the elderly, and provides qualitative neuropsychological data although administered by lay interviewers. The results obtained by a normaal group of 335 elderly persons aged between 60 and 100 years are discussed in relation to age and education level.