全文获取类型
收费全文 | 566篇 |
免费 | 33篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 22篇 |
妇产科学 | 4篇 |
基础医学 | 48篇 |
口腔科学 | 15篇 |
临床医学 | 120篇 |
内科学 | 96篇 |
皮肤病学 | 13篇 |
神经病学 | 15篇 |
特种医学 | 113篇 |
外科学 | 32篇 |
综合类 | 50篇 |
预防医学 | 26篇 |
眼科学 | 6篇 |
药学 | 24篇 |
1篇 | |
中国医学 | 2篇 |
肿瘤学 | 16篇 |
出版年
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2015年 | 31篇 |
2014年 | 43篇 |
2013年 | 24篇 |
2012年 | 12篇 |
2011年 | 28篇 |
2010年 | 19篇 |
2009年 | 23篇 |
2008年 | 23篇 |
2007年 | 26篇 |
2006年 | 28篇 |
2005年 | 18篇 |
2004年 | 14篇 |
2003年 | 14篇 |
2002年 | 14篇 |
2001年 | 14篇 |
2000年 | 11篇 |
1999年 | 15篇 |
1998年 | 15篇 |
1997年 | 16篇 |
1996年 | 18篇 |
1995年 | 22篇 |
1994年 | 25篇 |
1993年 | 12篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 6篇 |
1989年 | 11篇 |
1988年 | 9篇 |
1987年 | 7篇 |
1986年 | 10篇 |
1985年 | 21篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 3篇 |
1981年 | 10篇 |
1980年 | 8篇 |
1979年 | 1篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1976年 | 3篇 |
1957年 | 1篇 |
排序方式: 共有604条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
6.
Effects of Lymphocyte Isolation and Timing of Processing on Detection of CD127 Expression on T Cells in Human Immunodeficiency Virus-Infected Patients 下载免费PDF全文
Jeanette Higgins Julia A. Metcalf Randy A. Stevens Michael Baseler Martha C. Nason H. Clifford Lane Irini Sereti 《Clinical and Vaccine Immunology : CVI》2005,12(1):228-230
Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials. 相似文献
7.
Malyguine A Strobl SL Shafer-Weaver KA Ulderich T Troke A Baseler M Kwak LW Neelapu SS 《Journal of translational medicine》2004,2(1):9
Background
The desired outcome of cancer vaccination is to induce a potent T cell response which can specifically recognize and eliminate autologous tumor cells in vivo. Accordingly, immunological assays that demonstrate recognition of native tumor cells (tumor-specific) may be more clinically relevant than assays that demonstrate recognition of tumor protein or peptide (antigen-specific). 相似文献8.
Reorganization of human cortical maps caused by inherited photoreceptor abnormalities 总被引:3,自引:0,他引:3
We describe a compelling demonstration of large-scale developmental reorganization in the human visual pathways. The developmental reorganization was observed in rod monochromats, a rare group of congenitally colorblind individuals who virtually lack cone photoreceptor function. Normal controls had a cortical region, spanning several square centimeters, that responded to signals initiated in the all-cone foveola but was inactive under rod viewing conditions; in rod monochromats this cortical region responded powerfully to rod-initiated signals. The measurements trace a causal pathway that begins with a genetic anomaly that directly influences sensory cells and ultimately results in a substantial central reorganization. 相似文献
9.
Interleukin-2 induced immune effects in human immunodeficiency virus-infected patients receiving intermittent interleukin-2 immunotherapy 总被引:4,自引:0,他引:4
Kovacs JA Vogel S Metcalf JA Baseler M Stevens R Adelsberger J Lempicki R Hengel RL Sereti I Lambert L Dewar RL Davey RT Walker RE Falloon J Polis MA Masur H Lane HC 《European journal of immunology》2001,31(5):1351-1360
To characterize the immunological effects of intermittent IL-2 therapy, which leads to selective increases in CD4+ T lymphocytes in HIV-infected patients, 11 patients underwent extensive immunological evaluation. While IL-2 induced changes in both CD4+ and CD8+ cell number acutely, only CD4+ cells showed sustained increases following discontinuation of IL-2. Transient increases in expression of the activation markers CD38 and HLA-DR were seen on both CD4+ and CD8+ cells, but CD25 (a chain of the IL-2 receptor) increased exclusively on CD4+ cells. This increase in CD25 expression was sustained for months following discontinuation of IL-2, and was seen in naive as well as memory cells. IL-2 induced cell proliferation, but tachyphylaxis to these proliferative effects developed after 1 week despite continued IL-2 administration. It thus appears that sustained CD25 expression selectively on CD4+ cells is a critical component of the immunological response to IL-2, and that intermittent administration of IL-2 is necessary to overcome the tachyphylaxis to IL-2-induced proliferation. 相似文献
10.
Previously we have described the properties of store-operated channel currents (SOCs) in freshly dispersed rabbit portal vein smooth muscle cells. In addition to Ca2+ store depletion these SOCs could also be activated by α-adrenoceptor stimulation and diacylglycerol (DAG) via a protein kinase C (PKC)-dependent mechanism. In the present study we have investigated the effect of β-adrenoceptor stimulation on SOCs in rabbit portal vein myocytes. With whole-cell recording the selective β-adrenoceptor agonist isoprenaline reduced the current evoked by cyclopiazonic acid (CPA, sarcoplasmic/endoplasmic reticulum ATPase inhibitor) by over 85%. With cell-attached patch recording, bath application of isoprenaline produced a pronounced inhibition of SOC activity evoked by either CPA or the acetoxymethyl ester form of BAPTA (BAPTA-AM). SOC activity evoked by CPA, the DAG analogue, 1-oleoyl-acetyl- sn -glycerol (OAG) or the phorbol ester, phorbol-12,13-dibutyrate (PDBu) was also markedly inhibited by the adenylate cyclase activator, forskolin, and the cell-permeable non-hydrolysable analogue of cyclic adenosine monophosphate (cAMP), 8-Br-cAMP. With inside-out patches, bath application of PDBu evoked channel currents with similar properties to SOCs which were inhibited by over 90% by a catalytic subunit of protein kinase A (PKA) and by 8-Br-cAMP. Moreover bath application of PKA inhibitors, H-89, KT5720 and an inhibitory peptide to quiescent cell-attached or inside-out patches, activated channel currents with similar properties to SOCs. These data suggest that in rabbit portal vein myocytes, stimulation of β-adrenoceptors inhibits SOC activity via a cAMP-dependent protein kinase signal transduction cascade. In addition it is concluded that constitutive PKA activity has a profound inhibitory effect on SOC activity in this vascular preparation. 相似文献