全文获取类型
收费全文 | 886篇 |
免费 | 115篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 8篇 |
儿科学 | 21篇 |
妇产科学 | 9篇 |
基础医学 | 132篇 |
口腔科学 | 6篇 |
临床医学 | 115篇 |
内科学 | 243篇 |
皮肤病学 | 13篇 |
神经病学 | 112篇 |
特种医学 | 30篇 |
外国民族医学 | 1篇 |
外科学 | 132篇 |
综合类 | 3篇 |
一般理论 | 1篇 |
预防医学 | 84篇 |
眼科学 | 8篇 |
药学 | 27篇 |
中国医学 | 3篇 |
肿瘤学 | 55篇 |
出版年
2023年 | 5篇 |
2022年 | 26篇 |
2021年 | 43篇 |
2020年 | 26篇 |
2019年 | 43篇 |
2018年 | 56篇 |
2017年 | 30篇 |
2016年 | 42篇 |
2015年 | 41篇 |
2014年 | 38篇 |
2013年 | 41篇 |
2012年 | 61篇 |
2011年 | 58篇 |
2010年 | 38篇 |
2009年 | 28篇 |
2008年 | 30篇 |
2007年 | 44篇 |
2006年 | 35篇 |
2005年 | 31篇 |
2004年 | 30篇 |
2003年 | 25篇 |
2002年 | 23篇 |
2001年 | 23篇 |
2000年 | 25篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 16篇 |
1991年 | 10篇 |
1990年 | 16篇 |
1989年 | 8篇 |
1988年 | 10篇 |
1987年 | 7篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1967年 | 3篇 |
1958年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有1003条查询结果,搜索用时 15 毫秒
1.
Barouyr Baroudjian Dimitri Arangalage Stefania Cuzzubbo Baptiste Hervier Celeste Lebbé Gwenael Lorillon 《Expert review of anticancer therapy》2019,19(3):209-222
Introduction: Immune checkpoint inhibitors (ICI) are now a standard of care in the treatment of many cancers leading to durable responses in patients with metastatic disease. These agents are generally well tolerated but may lead to the occurrence of immune-related adverse events (irAEs). As any organ may be affected, clinicians should be aware of the broad range of clinical manifestations and symptoms and keep in mind that toxicities may occur late, at any point along a patient’s treatment course. Although the most common irAEs are rarely severe, some of them may be associated with great morbidity and even become life-threatening. The rate of occurrence, type and severity of irAEs may vary with the type of ICI; thus, grade 3 and 4 irAEs are reported in more than 55% of patients treated with the combination of ipilimumab 3 mg/kg and nivolumab 1 mg/kg.
Area covered: This review presents the management of irAEs resulting from checkpoint blockade, with a focus on rare irAEs.
Expert commentary: With the development of immuno-oncology and the expanding role of ICI, physicians have learnt to diagnose and treat most of the irAEs that can occur. This review provides an overview of current guidelines, previously published studies and our multidisciplinary team based practices. 相似文献
2.
A case is reported of allergy to ketamine substantiated 2 years after the accident. The female patient had ketamine for the removal of a mole. During this procedure, she developed a generalized rash and laryngospasm; the use of adrenaline was required. Since that time, although no confirmatory investigations had been carried out, she had been given a paper identifying Ketalar as being the involved drug. Investigations were carried out after she had been given a further anaesthetic (without using ketamine) 2 years after the accident. These confirmed an allergic reaction to ketamine, and not to the solvent, chlorobutanol. 相似文献
3.
4.
Ontogenesis of angiotensin-I converting enzyme in human kidney 总被引:4,自引:0,他引:4
F Mounier N Hinglais M Sich F Gros M Lacoste Y Deris F Alhenc-Gelas M C Gubler 《Kidney international》1987,32(5):684-690
The kidney distribution of angiotensin-I converting enzyme (ACE) was studied in 14 fetuses (11 to 30 weeks old) and 7 children (2 days to 13 years old) by immunohistochemistry using specific antibodies to human kidney ACE. Immunohistochemical techniques included indirect immunofluorescence on cryostat sections of frozen tissue, immunoperoxidase and immunofluorescence of fixed tissue embedded in Paraplast, and immunoelectron microscopy. The ACE distribution in the fetal kidneys was independent of the age of the fetus. ACE was detected in two locations: 1) on the basolateral membranes and primary apical microvilli of epithelial cells from early differentiating proximal tubules; the labeling was intense in brush borders of fully developed proximal tubules; and 2) on glomerular endothelial cells; cells were lined by reaction product as soon as capillaries invaded the inferior cleft of the S-shaped body. Tubular ACE distribution was identical in the postnatal kidneys. The staining of the glomerular endothelium was extremely inconstant. The presence of ACE in proximal tubular cells and glomerular endothelial cells at the beginning of nephron differentiation may indicate that it is involved in the development of nephron function and renal hemodynamic. 相似文献
5.
Karayan J.; Thomas D.; Lacoste L.; Dhoste K.; Ricco J. B.; Fusciardi J. 《British journal of anaesthesia》1996,76(3):459-460
We have evaluated the efficacy of the delayed forced air warming during
abdominal aortic surgery in 18 patients. Patients were allocated randomly
to one of two groups: the control group (n = 9) received no intraoperative
warming device; the Bair-Hugger group (n = 9) had active skin surface
warming with an upper body cover. The device was activated when core
temperature decreased to less than 36 degrees C. The reduction in core
temperature was 0.6 degrees C during the first hour after induction and 0.4
degrees C during the second hour in both groups. In the control group, core
temperature continued to decrease until the end of surgery, whereas in the
Bair-Hugger group, the reduction in core temperature stopped after 1 h of
warming, and then rewarming began. At the end of surgery, core temperature
in the Bair- Hugger group was similar to core temperature before induction,
and was higher than core temperature in the control group (P < 0.003).
相似文献
6.
Farida Daïkha-Dahmane Françoise Narcy Marc Dommergues Mireille Lacoste Agnes Beziau Marie-Claire Gubler 《Pediatric nephrology (Berlin, Germany)》1997,11(3):267-273
An alteration in cell/matrix interactions is one of the suggested mechanisms leading to cyst formation in polycystic kidney
diseases. Most of these interactions are mediated by β1-integrins, a subfamily of integrin receptors, formed by the association
of the β1-chain with different α-subunits. To date, no study on α-integrin subunit distribution during the early stages of
cyst development has been reported. Using immunofluorescence, we analyzed the distribution of α-integrin subunits (α1, α2,
α3, α5, and α6) and basement membrane proteins in kidneys of fetuses with autosomal dominant (ADPKD) or autosomal recessive
polycystic kidney disease (ARPKD). The distribution was compared with that observed in normal fetal and post-natal kidneys,
and in fetal cystic dysplasia and Meckel syndrome. Marked increase in α1-integrin staining was observed in normal and cystic
collecting duct cells of both polycystic diseases (PKD), compared with normal and cystic controls. The distribution of integrin
subunits α2, α3, and α6 was irregular in cyst epithelial cells of PKD and cystic controls. The increased expression of the
α1-subunit specifically observed in PKD collecting duct cells may be an early consequence of the genetic defect in ARPKD.
In ADPKD it parallels the reported expression of polycystin, the protein product of PKD1. The irregular expression of α2,
α3, and α6 integrin subunits observed in all types of cysts suggests that cell/matrix interactions are altered early and may
participate in the development of cysts, perhaps by contributing to the deregulation of cell survival in cystic diseases.
Received May 28, 1996; received in revised form October 2, 1996; accepted October 25, 1996 相似文献
7.
Louis Journeau Marc-Antoine Pistorius Ulrique Michon-Pasturel Marc Lambert Francois-Xavier Lapébie Alessandra Bura-Riviere Philippe de Faucal Patrick Jego Quentin Didier Cécile Durant Geoffrey Urbanski Baptiste Hervier Claire Toquet Christian Agard Olivier Espitia 《Autoimmunity reviews》2019,18(5):476-483
8.
Linda Wittkop Daniel Commenges Isabelle Pellegrin Dominique Breilh Didier Neau Denis Lacoste Jean-Luc Pellegrin Geneviève Chêne François Dabis Rodolphe Thiébaut 《BMC medical research methodology》2008,8(1):68
Background
Principal component analysis (PCA) and partial least square (PLS) regression may be useful to summarize the HIV genotypic information. Without pre-selection each mutation presented in at least one patient is considered with a different weight. We compared these two strategies with the construction of a usual genotypic score. 相似文献9.
Effect of nutritional constraints on the biosynthesis of the components of the phosphoenolpyruvate: sugar phosphotransferase system in a fresh isolate of Streptococcus mutans. 总被引:2,自引:2,他引:2
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A procedure for the purification of enzyme I (EI) and the protein HPr, the general components of the phosphoenolpyruvate:sugar phosphotransferase system, from Streptococcus mutans serotype c is presented. The method was also applied successfully to the purification of EI and HPr from Streptococcus salivarius, Streptococcus sobrinus, and Streptococcus sanguis. Using specific antibodies obtained against the proteins purified from S. mutans DR0001, we determined quantitatively by rocket electrophoresis the cellular levels of EI and HPr in a freshly isolated strain of S. mutans grown under various conditions in continuous culture. The activity of a few specific EIIs was also determined by an in vitro phosphorylation test. Results indicated that maximum EII activities for glucose, mannose, and 2-deoxyglucose were obtained under conditions of glucose limitation, at pH 7.0 and low dilution rate (D = 0.057/h). Increasing the amount of glucose or the dilution rate (D = 0.40/h) or decreasing the pH from 7.0 to 5.5 resulted in a 1.4- to 24-fold decrease in these activities. The EII activity for fructose was not influenced by the growth conditions in the same way as the other EIIs. The fructose EII was highest at pH 5.5 and at high dilution rate under conditions of glucose or nitrogen limitation and was always repressed at pH 7.0 and at low dilution rates. The intracellular levels of EI were also dependent on the growth conditions. The highest concentration (0.65 nmol/mg of protein) was observed in cells grown under glucose limitation at pH 7.0 and high dilution rate, and the lowest concentration (0.12 nmol/mg of protein) was found in cells grown under glucose excess at pH 7.0 and high dilution rate. The other general component of the phosphoenolpyruvate:sugar phosphotransferase system, the protein HPr, was not influenced significantly by varying growth conditions. 相似文献
10.
Bonnet F Savès M Morlat PH Droz C Chêne G Peuchant E Bernard N Lacoste D Salamon R Beylot J 《Journal of clinical immunology》2002,22(2):75-82
We assessed the correlations between some plasma markers of immune activation (soluble receptors of interleukin 2 (sIL2-R) and TNFp75 (sTNFII-R) and usual markers of HIV infection in patients treated with protease-inhibitors (PI). Forty-six PI-naive HIV-1-infected adults were included in a 1-year prospective cohort from the initiation of a PI-containing regimen (M0). Measurements of CD4+cell count, plasma HIV-RNA, sIL2-R and sTNFII-R were performed at M0, M6, and M12. The evolution of sIL2-R from baseline to M12 was significantly different between immunological responders (IR) (CD4+count above 200/mm3 for subject having less than 200 CD4 +/mm3 at inclusion, or increase of at least 50 CD4+/mm3 for others) (58 UI/ml) and non-IR (+28 UI/ml) (P =0.01). The evolution of sTNFII-R between M0 and M12 was significantly different between virological responders (VR) (plasma HIV-1 RNA less than 500 copies/ml at M12) (–2.5 ng/ml) and non-VR (+0.2 ng/ml) (P =0.02). Our study shows significative correlations between the evolutions of soluble interleukin-2 and TNFR-II receptors and those of CD4+T-lymphocytes or HIV-RNA responses in patients under HAART. 相似文献