The antigenic properties of hepatitis A virus particles possessing different physicochemical characteristics]

A modified enzyme immunoassay based on adsorption of antihepatitis A virus (HAV) IgG-HRPO conjugate and monoclonal antibodies to HAV were used to investigate antigenic differences between mature HAV virions and subviral particles with different buoyant densities in CsCl produced in HAV-infected cells. The mature virions (1.34 g/cm3) appeared to have common antigenic determinants with subviral particles (1.20, 1.27, and 1.30 g/cm3) and possess some additional determinants. Nevertheless, both subviral particles and mature virions induced antibodies capable of neutralizing HAV infectivity in tissue culture.     

The effect of naloxone on the preoperative gastric volume and pH

The effect of 4 mg oral naloxone on preoperative gastric volume and pH of gastric aspirate was studied in a double-blind, randomized study. Twenty patients received 10 ml of naloxone (4 mg) mixed with 10 ml of orange juice, and 20 patients received 10 ml of isotonic saline mixed with 10 ml of orange juice, 2 h before surgery. Gastric content was obtained immediately after intubation of the trachea. No significant difference in gastric volume and pH of gastric aspirate was found between the two groups. It is concluded that naloxone does not affect gastric emptying and gastric acid secretion to a degree great enough to protect against aspiration of gastric contents into the lungs.     

TLC Determination of Cyclophosphamide Degradation Products in Parent Substance and Drug Preparations

Pharmaceutical Chemistry Journal -     

Interactions between the genetic transfer-inhibiting systems finU and finV in polyplasmid complexes ofEscherichia coli K-12 cells

The inhibition of conjugative transfer of derepressed plasmids was appreciably increased in polyplasmid complexes ofEscherichia coli K-12 cells containing two repressed conjugative plasmids with the finV system of genetic transfer regulation, in comparison with the individual effects of each of the two plasmids. Combination of two plasmids with the finU system or of plasmids with the finU and finV systems did not result in such an increase of inhibitory activity. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 12, pp. 619–622, December, 1995 Presented by T. T. Berezov, Member of the Russian Academy of Medical Sciences