新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.     

奥氮平治疗老年期难治性精神分裂症临床分析

目的：评价奥氮平治疗老年期难治性精神分裂症的疗效与安全性. 方法：用奥氮平治疗老年期难治性精神分裂症患者62例.疗程16周.采用阳性与阴性症状量表（PANSS）评价临床疗效,副反应量表（TESS）评价不良反应. 结果：PANSS总分、阳性症状、阴性症状及一般精神病理分在治疗前后差异有显著性.有效率为58.1%,显效率为32.3%,未见严重的不良反应. 结论：奥氮平对老年期难治性精神分裂症安全有效,服用方便.     

腹腔镜下胆总管切开取石一期缝合术后胆漏相关危险因素分析

目的 探讨腹腔镜下胆总管切开取石一期缝合术后胆漏的独立危险因素,并做出相应预防策略;为临床行腹腔镜下胆道切开取石一期缝合提供帮助,减少术后胆漏的发生。方法 回顾性分析我院肝胆外科在2019年至2023年收治的102例胆总管结石并行腹腔镜下胆道切开取石一期缝合手术患者的临床资料,根据患者术后是否发生胆漏分为胆漏组(n=18)和非胆漏组(n=84)。分析所有患者的一般资料、血清白蛋白、结石数目、胆总管直径、手术时间、术中出血量、缝合方式、主刀医生手术经验。通过单因素分析及多因素Logistic回归分析探索术后发生胆漏的危险因素,将危险因素指标纳入受试者工作特征(ROC)曲线进行分析。结果 102例患者中18例患者出现胆漏,包括16例A级胆漏以及2例B级胆漏,对两组患者的临床资料行单因素及多因素Logistic回归分析结果显示：术者经验<30例,术前血清白蛋白低,使用连续性缝合的胆管缝合方式是患者术后发生胆漏的独立危险因素,差异有统计学意义(均P<0.05)。受试者工作特征(ROC)曲线分析结果：术前白蛋白水平界值为37.45 g/L(P<0.05)。结论 术前血清白蛋白<37.45 g/L,连续缝合胆总管切口,术者手术经验<30例可作为预测腹腔镜下胆总管切开取石一期缝合术后发生胆漏的重要理论依据。     

软组织移植用于治疗正畸后牙龈退缩的临床疗效分析

目的:用软组织移植的方法治疗正畸后牙龈退缩,通过统计相关临床指标来评估软组织移植治疗正畸治疗后牙龈退缩的临床效果.方法:收集正畸治疗后发生牙龈退缩的患者12例,均为MillerⅠ类、MillerⅡ类牙龈退缩,采用冠向复位瓣+结缔组织移植或游离龈移植的方法治疗牙龈退缩,在基线和术后1年随访时分别记录每颗牙牙龈退缩深度(RD)、角化龈宽度(KTW)、根面覆盖率(RC),探诊深度(PD),采用视觉模拟评分法(VAS)评估患者对手术的满意度,计算各项临床指标平均值变化.结果:12例患者的平均基线RD为(3.7±0.9)mm,术后1年平均RD为(0.3±0.9)mm;术后1年患者的平均RC为(97.1±6.3)％,大部分牙齿得到完全根面覆盖;患者基线和术后1年平均KTW为(0.8±0.3)mm和(3.8±0.6)mm,角化龈增加明显;PD从基线(1.5±0.5)mm减少到(1.2±0.5)mm;术后1年患者满意度评分为(9.6±0.6)分,大部分患者对术后效果感到满意且根面敏感情况好转.结论:正畸治疗后牙龈退缩的患者采用冠向复位瓣+结缔组织移植或游离龈移植的方法均可取得良好临床效果,患者根面敏感情况得到明显改善且满意度高.     

三维牵引结合电针夹脊穴治疗腰椎间盘突出症的观察及护理

腰椎间盘突出症是临床常见疾病之一,约1／5的腰腿痛病人患腰椎间盘突出症。其诊断随着CT、M砌等影像技术的普及应用日臻完善。然而,治疗该病目前还没有较理想的方法。非手术疗法中,多数患者通过传统的骨盆牵引、推拿、理疗等能取得比较满意的疗效,但疗程较长,尚存许多不足。我科采用DFQ-300型多功能牵引床以三维脊柱旋转牵引结合电针夹脊穴技术治疗腰椎间盘突出症58例,取得满意疗效。     

胡须保健,不可小视

有关男人胡子的作用,人们争论了几个世纪,其中一个有趣的说法是,胡须是天然的领巾,用来保护脆弱的喉部和取暖。其实胡须没有那么大的作用。胡须之于男人而言,是男性的旗帜,对于一个有分量的男人,胡须是一种传达个性的     

城市生活垃圾转运站经济合理性研究

应用技术经济学理论,分别计算建与不建垃圾转运站时各自的固定投资以及运行成本,考虑资金时间价值,讨论其经济合理性,形成城市生活垃圾转运站经济合理性判定模型,并将该模型应用到重庆主城区生活垃圾转运站选址、建设的经济分析中.     

新城疫病毒D817对裸小鼠人结肠癌移植瘤的抑制作用

Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.     

肺炎——春季须重防

春天，是肺炎的高发季节。初春伊始，季节转换，日温差、气压、湿度变化大，空气干燥，细菌活跃，最易导致呼吸道感染，而蔓延成支气管炎和肺炎，严重时还可出现呼吸困难和口唇、指甲绀紫。     

广西医科大学2007年新生H3N2亚型流感病毒抗体调查

目的：调查广西医科大学2007年新生H3N2亚型流感病毒抗体,了解健康青年人群血清中H3N2亚型流感病毒抗体的水平,从而预测广西H3N2流感病毒引起的流感流行的趋势。方法：抽取广西医科大学2007年新生血清168份,通过血凝抑制实验进行抗体检测。结果：以血凝抑制抗体滴度≥1：10为H3N2亚型流感病毒抗体阳性,H3N2亚型流感病毒抗体阳性率为26．19％,抗体滴度≥1：40的占阳性的22．73％。结论：H3N2亚型流感病毒引起的流感在广西有流行,但流行强度不高,抗体滴度水平对人群的保护能力较低。