胸腔内注入顺铂联合滑石粉治疗恶性胸腔积液疗效比较

目的 探讨胸腔内注入顺铂（DDP）联合滑石粉治疗恶性胸腔积液的疗效。方法 病理确诊的恶性胸腔积液58例,经胸腔插管引流术排除胸水后,随机分为二组,开始均胸腔内注入80mg顺铂,治疗组（A组,30例）1周后重复一次,并注入3％滑石粉混悬液100ml;对照组（B组,28例）1周后使重复一次,观察疗效、生活质量、生存率及毒副反应。结果 治疗组总有效率80％,较对照组46％,差异有显著性（P〈O．01）。治疗组0．5、1年的生存率分别高于对照组,差异有显著性。结论 胸腔内联合注入DDP和滑石粉治疗恶性胸腔积液是一种有效的、经济的、副反应少的方法。     

静电纺壳聚糖/胶原蛋白复合纳米纤维的细胞相容性

BACKGROUND： Due to the lack of autograft transplant rejection, Schwann cells （SCs） can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be induced to produce dopaminergic neurons. The therapeutic effects of co-grafts of SCs and neural stem cells （NSCs） deserves further study and verification in Parkinsonian animal models. OBJECTIVE： To investigate the effects of Schwann cells and mesencephalic NSC co-grafts in Parkinsonian animal models on animal behavior and histology. DESIGN： Randomized controlled experiment. SETTING： Fudan University; Institute of Neuroscience, Chinese Academy of Sciences. MATERIALS： The following animals were obtained from the Experimental Animal Center, Shanghai Institute for Biological Science, Chinese Academy of Sciences： 5 Sprague-Dawley rats, embryonic day （E） 13-16; 16 neonatal Sprague-Dawley rats, postnatal day 1-3; and 18 adult SD rats of both genders. Animal experimentation met animal ethical approval. METHODS： The experiment was performed at the Department of Anatomy, Histology and Embryology, Shanghai Medical Center, Fudan University from September 2005 to January 2007. The mesencephalic NSCs were obtained from the brains of SD rats at E 13-16, and SCs were harvested from the sciatic nerves of neonatal rats at day 1-3. Hemiparkinsonian rats （n =18） were selected for transplantation after estimating rotational behavior in response to apomorphine and were randomly assigned to three groups： control group, NSC group, and co-graft group. There were 6 rats in each group. Either phosphate buffered saline （PBS）, NSCs, or SCs plus NSCs were transplanted into the right neostriatum of Parkinsonian rats, respectively. MAIN OUTCOME MEASURES： （1） Rotational behavior was induced by apomorphine （0.05 mg/kg, i.p.） 2, 4, 6, 8, and 10 weeks after transplantation, and the number of rotations were counted. （2） Differentiation and survival of dopaminergic neurons in the right neostr     

静电纺壳聚糖/胶原蛋白复合纳米纤维的细胞相容性

目的:静电纺是一种使带电荷的聚合物溶液或熔体在静电场中射流来制备聚合物纳米级纤维的加工方法,采用此种技术制备壳聚糖/胶原蛋白复合纳米纤维,并观察其细胞相容性.方法:实验于2006-11/2007-10在东华大学化学化工与生物工程学院生物材料与组织工程实验室完成.①支架材料制备:以六氟异丙醇/三氟乙酸为溶剂体系,采用静电纺制备复合纳米纤维,其中壳聚糖/胶原蛋白的质量比分别为100:0,80:20,50:50,20:80与0:100.②细胞相容性观察:体外接种猪髋动脉内皮细胞,苏木精-伊红染色法观察细胞形态,MTT法检测细胞黏附和增殖情况.结果:猪髋动脉内皮细胞在壳聚糖/胶原蛋白复合纤维表面贴附牢固,外形饱满,多呈长梭形,具有良好的生长形态;MTT法结果显示纳米纤维能够有效地促进内皮细胞在材料表面的黏附和增殖,质量比为20:80材料组细胞黏附、增殖能力最强,其次为50:50组.结论: 静电纺壳聚糖/胶原蛋白复合纳米纤维具有良好的细胞相容性,可望成为一种新型的组织工程支架材料.     

甲亢性心脏病致房室分离1例

1 临床资料及心电图分析 患者女性,56岁,临床诊断:甲状腺机能亢进,甲亢性心脏病。附图为Ⅰ与V_1导联非同步记录,以V_1导联并结合格形图分析如下:窦性心律,心率约85次/min,大多数窦P不能下传心室;交界区有一活跃的节律点。频率约100次/min,为交界区非阵发性心动过速(非阵速),其冲动逆行传导受阻,前向下传心室时形成高矮交替的不完全性右束支阻滞型QRS。R_(4、9)为窦P下传     

头孢噻肟和鱼腥草联合治疗下呼吸道感染

头孢噻肟和鱼腥草联合应用，治疗下呼吸道感染31例，并与头孢噻肟联合双黄连治疗者对照，有效率分别为87.1%和83.9%，两者比较无显著差异(P＞0.05)。但对慢阻肺肺部感染者，治疗组优于对照组。