疑难病例析评 第46例 发热-匐行疹-上臂水肿性疼痛性红斑块

颚口线虫病在我国尚属少见。棘颚口线虫偶尔寄生于人体。该虫致病部位极为广泛，几乎遍及全身，受累部位可间歇性出现移行性肿块，常伴有局部水肿、疼痛，虫体移行可引起机械性损伤，如虫体停留在某一寄生部位，可形成以虫体为中心的脓肿及隧道样损害。该虫较易侵入眼、脑，并可引起嗜酸性粒细胞增多性脑脊髓炎，后果严重，甚至可导致死亡。本文详细地介绍了发生在北京地区首例病人的临床资料，并结合国内外文献，对该病进行了较为全面的阐述。该病例提示：(1)医务工作者应加强对该病进行了解。(2)临床遇有可疑表现者，尤其有生食本虫第二中间宿主或转续宿主者，应考虑到本病的可能，并做进一步检查(例如：嗜酸性粒细胞计数、血红细胞沉降率等)，能在病损部位查见虫体者即可以确诊。皮内实验和血清学试验亦可做辅助诊断。(3)近年来随着人们饮食习惯的改变，喜食烧、烤、涮或生食各种动物的人群增多，食源性寄生虫病病人亦有增多趋势，本病正是诸多食源性寄生虫病之一。     

疑难病例析评 第46例 发热—匐行疹—上臂水肿性疼痛性红斑块

颚口线虫病在我国尚属少见。棘颚口线虫偶尔寄生于人体。该虫致病部位极为广泛 ,几乎遍及全身 ,受累部位可间歇性出现移行性肿块 ,常伴有局部水肿、疼痛 ,虫体移行可引起机械性损伤 ,如虫体停留在某一寄生部位 ,可形成以虫体为中心的脓肿及隧道样损害。该虫较易侵入眼、脑 ,并可引起嗜酸性粒细胞增多性脑脊髓炎 ,后果严重 ,甚至可导致死亡。本文详细地介绍了发生在北京地区首例病人的临床资料 ,并结合国内外文献 ,对该病进行了较为全面的阐述。该病例提示 :(1)医务工作者应加强对该病进行了解。 (2 )临床遇有可疑表现者 ,尤其有生食本虫第二中间宿主或转续宿主者 ,应考虑到本病的可能 ,并做进一步检查 (例如 :嗜酸性粒细胞计数、血红细胞沉降率等 ) ,能在病损部位查见虫体者即可以确诊。皮内实验和血清学试验亦可做辅助诊断。(3)近年来随着人们饮食习惯的改变 ,喜食烧、烤、涮或生食各种动物的人群增多 ,食源性寄生虫病病人亦有增多趋势 ,本病正是诸多食源性寄生虫病之一。     

Ursolic acid induces neural regeneration after sciatic nerve injury

In this study,we aimed to explore the role of ursolic acid in the neural regeneration of the injured sciatic nerve.BALB/c mice were used to establish models of sciatic nerve injury through unilateral sciatic nerve complete transection and microscopic anastomosis at 0.5 cm below the ischial tuberosity.The successfully generated model mice were treated with 10,5,or 2.5 mg/kg ursolic acid via intraperitoneal injection.Enzyme-linked immunosorbent assay results showed that serum S100 protein expression level gradually increased at 1-4 weeks after sciatic nerve injury,and significantly decreased at 8 weeks.As such,ursolic acid has the capacity to significantly increase S100 protein expression levels.Real-time quantitative PCR showed that S100 mRNA expression in the L4-6 segments on the injury side was increased after ursolic acid treatment.In addition,the muscular mass index in the soleus muscle was also increased in mice treated with ursolic acid.Toluidine blue staining revealed that the quantity and average diameter of myelinated nerve fibers in the injured sciatic nerve were significantly increased after treatment with ursolic acid.10 and 5 mg/kg of ursolic acid produced stronger effects than 2.5 mg/kg of ursolic acid.Our findings indicate that ursolic acid can dose-dependently increase S100 expression and promote neural regeneration in BALB/c mice following sciatic nerve injury.     

寄生虫病防治中的一些新问题

寄生虫病是我国最常见的疾病,新中国成立以后,开展了大规模的防治工作,取得了很大的成就,已基本上控制了黑热病与丝虫病的流行,其他寄生虫病也已明显减少。但随着我国改革开放的深入,人民生活水平的不断提高,寄生虫病防治中也出现一些新问题值得我们注意。 1.以往少见的寄生虫病增多了: 广州管圆线虫病(angiostrongyliasis cantonensis)在东南亚及我国台湾省较多见,但在我国大陆仅有个案报告。近年来引进了一些巨螺如褐云玛瑙螺、瓶螺(也称福寿螺,俗称苹果蜗螺)饲养,并进入     

Enteroaggregative Escherichia aoliisolated from Chinese diarrhea patients with high-pathogenicity island of Yersinia''''xs involved in synthesis of siderophore yersiniabactin

AIM: To investigate the distribution of 12 high-pathogenicity island(HPI) genes and the relation between HPI genes and expression of yersiniabactin(Ybt) in enteroaggregative E.coli(EAggEC) isolated from Chinese diarrhea patients. METHODS: The distribution of 12 HPI genes was investigated by PCR and DNA hybridization in two prototype strains of EAggEC, EAggEC 17-2, EAggEC 042, and 6 clinical EAggEC isolates from China. The production of siderophore Ybt in HPI-positive strains was detected by reporter gene bioassay to determine the relation between HPI genes and expression of Ybt. Flow cytometry was used to detect fluorescent signal of the reporter strain that could designate production of Ybt. RESULTS: Seven strains were HPI-positive and one strain was HPI-negative. Six of the seven HPI-positive strains were inserted into asnT-tRNA site. Moreover, seven EAggEC HPI-positive strains revealed enhanced fluorescence signal but the EAggEC HPI-negative strain did not. However, there was a difference in Ybt expression condition and level among these seven EAggEC HPI-positive strains. Although UFT073 strain, the prototype strain of uropathogenic E.coli(UPEC), carried the complete HPI core part, we did not detect the expression of Ybt in it. CONCLUSION: EAggEC HPI-positive strains can express the Ybt system, but the presence of HPI core part does not mean the functional expression of Ybt.     

Correlation between immune-related adverse events and long-term outcomes in pembrolizumab-treated patients with unresectable hepatocellular carcinoma: A retrospective study

BACKGROUND Although immune checkpoint inhibitor(ICI) therapy has improved the prognosis of unresectable hepatocellular carcinoma(HCC), it has also resulted in unique immune-related adverse events(irAEs). The relationship between irAE and treatment outcomes in ICI-treated unresectable HCC patients remains unknown.AIM To elucidate the correlation between immune-related toxic effects and prognosis in patients with unresectable HCC treated with pembrolizumab.METHODS From March 2019 to February 2021,...     

Two new terpenoids from Reduning Injection

Objective: To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning(RDN) Injection.Methods: In this study, the active fraction of RDN Injection was screened by the LPS-induced mouse endotoxin shock model. The chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins, silica gel, ODS columns and reverse phase MPLC and HPLC repeatedly, and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS, NMR, ECD) and chemical methods.Meanwhile, we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-α production in LPS stimulated RAW 264.7 macrophages.Results: The 95% ethanol eluate of RDN Injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection. A novel iridoid, named jasminoide A(1), and a new guaiane sesquiterpenoid, named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2), were isolated from Reduning injection, and compound 2 can inhibit TNF-α production with IC50 values of 72.24 μmol/L.Conclusion: In this study, two new terpenoids were isolated from Reduning Injection, and compound 2 showed inhibitory activity against LPS-activated TNF-α production in RAW 264.7 cells in vitro.     

P20 Deepening the Reform of Pharmacological Experiments, Cultivating the Sense of Innovation in Students

The pharmacological experiment is an important and direct way to improve students＇ practical ability. Through the lab experiments, the students can strengthen their understanding of pharmacological effects or side effects ; learn the basic pharmacological ideas related to drug evaluation and develop their sense of innovation. Thus, we need to reform the design of the experiments.     

Effect of NS398 on inducing apoptosis and down-regulating Bcl-2 protein in human osteosarcoma cell MG-63 line

Objective： This study investigated the effect of NS398 on anti-proliferation and the mechanism of inducing apoptosis of human osteosarcoma cell MG-63 line in vitro. Methods： Growth suppression was detected by MTT method. Special morphological changes of apoptosis were observed by transmission electron microscopy （TEM） and DNA fragments electrophoresis. The apoptotic rates were quantified by flow cytometry （FCM）. And Bcl-2 protein level were detected by Western Blotting assay. Results： Different concentration NS398 inhibited the cell growth. Through TEM and DNA electrophoresis, the special morphological changes were observed after 24, 48 and 72 h treatment with NS398. The apoptotic rates of MG-63 cells treated with NS398 were respectively, significantly higher than that of the control group（ P 〈0.01 ）. Western blot assay showed that NS398 reduced Bcl-2 protein expression. Conclusion： NS398 suppressed the proliferation of human osteosarcoma cell MG-63 line and induced apoptosis. The mechanism may be associated with down-regulation expression level of bcl-2 protein.     

提高医院维修管理系统的效率

医院的维修管理是牵制医院行政管理费用的重要领域,对提高医院维修管理系统(HM MS)效率的方法曾进行了许多研究。为使医院维修管理系统取得成功,必须着重强调以下八个方面:(1) 组织与职能;(2) 工作管理文件;(3) 计划;(4) 日程安排;(5) 物资管理;(6) 预防性维修;(7) 工作的评价;(8) 电脑应用。组织与职能: 无论是保健机构或工厂,其维修部门的组织结构必须确定其基本关系。对医院的维修部门最重要的两种建制是职能型和地理型。前者是根据相似工作性质组织的,后者则根据完成工作的地点来决定的。维修管理人员是医院维修部门各项活动和职能的主要负责人,其名称不同,有称服务部主住、医院工程师等。维修管理人员的任务是有效地组织、计划、指导并管理工作人员,材料及其他资源。