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Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P<0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P<0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P>0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM. 相似文献
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Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P<0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P<0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P>0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM. 相似文献
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Objective To investigate the effect of SiO2 on the expression of matrix metalloproteinase-9 (MMP-9)and tissue inhibitor of metalloproteinase-1 (TIMP-1)in human silicofic alveolar macrophages (AM).Methods Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 3h,6h,12h,18h,24h and 36h.The expression of the MMP-9 in the AM were detected with zymography and immunological method and the expression of the TIMP-1 in the AM with immunological method.Results The expressions of MMP-9 in the AM increased clearly along with the time,reached peak at 24 h when detected with zymography (average optical density:3.061±0.153 vs 2.851±0.164,P<0.05);and reached peak at 18h when detected with immunological method (average optical density:0.386±0.037 vs 0.322±0.034,P<0.05).The expression of the TIMP-1 in the AM did not vary when detected with immunological method (P>0.05).Conclusion SiO2 may affect the expression of MMP-9 and MMP-9 activity in the cultured AM. 相似文献
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笔者自 1996年 3月~ 2 0 0 1年 3月 ,运用针刺、中药并用治疗“脑萃中” 33例 ,现报道如下。1 对象与方法1 1 对象全部病例均符合 1986年全国中、西医脑血管疾病诊断标准 ,并经过颅脑CT片报告证实 ,33例中男性 2 3例 ,女性 10例 ;年龄 :4 5~ 73岁 ,平均年龄5 9岁 ,超过 6 0岁者为 15例 ,占 4 9 5 % ,发病时间8~ 14 8h内。1 2 中医诊断分型中风 :中经络 2 3例 ;中脏腑 10例 ,其中阴闭 4例 ,阳闭 5例 ,脱证 1例。西医诊断 :缺血性脑血管病 2 3例 ,其中多发性、多部位 5例 ,区限性 8例 ,双基底节区 6例 ,腔隙性 4例。出血性脑血管病 10… 相似文献
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我们自1989年以来,用自拟眩晕八味汤治疗50例眩晕病患者,均获卓效,现介绍如下:一、方药组成党参15g,白术10g,山药15g,川芎15g,菊花20g,山萸肉12g,夏枯草12g,钩藤10g。二、临床运用眩晕伴恶心呕吐者加竹茹、代赭石、半夏、生姜;耳鸣重听者加郁金、九节菖蒲;痰浊内盛者加天麻和二陈汤;气血两亏者加黄芪、当归、阿胶;肾精不足 相似文献
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目的研究姜黄素对人肺成纤维细胞(human lung fibroblasts,HLFs)体外生长、细胞周期及凋亡的影响。方法应用CCK-8、Annexin-V/PI、PI流式细胞分析术、免疫印迹法,检测姜黄素对细胞的增殖、凋亡、细胞周期和Bax蛋白表达的影响。结果姜黄素对HLFs的增殖有抑制作用,可促进HLFs的早期凋亡,对细胞周期可产生G2/M期阻滞,增加HLFs的Bax蛋白的表达。结论姜黄素可抑制HLFs的增殖并能诱导其凋亡,其机制可能与细胞的G2/M期阻滞及凋亡相关蛋白的表达增强有关。 相似文献
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AcSDKP对TGF-β 1诱导的大鼠心脏成纤维细胞胶原蛋白合成及表达的调节作用 总被引:4,自引:0,他引:4
目的 探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对转化生长因子β1(TGF-β1)诱导的大鼠心脏成纤维细胞胶原合成的调节作用.方法 差速贴壁法获取大鼠心脏成纤维细胞;采用3H-脯氨酸掺入法检测心脏成纤维细胞胶原蛋白的合成.采用免疫细胞化学染色和Western blotting法检测心脏成纤维细胞Ⅰ型与Ⅲ型胶原蛋白的表达.结果 随着TGF-β1浓度的增加(1μg/L, 2.5μg/L,5μg/L,7.5μg/L),细胞脯氨酸含量(CPM值)逐渐增加(分别为147.6±10.2,229.2±16.4,427.0±40.6,454.8±26.1),分别是对照组(CPM值为91.6±9.8)的1.61倍、2.50倍、4.66倍、4.97倍,差异均有显著性(P<0.05).当加入不同浓度的AcSDKP(10-10mol/L,5×10-10mol/L, 10-9mol/L, 10-8mol/L)时,细胞脯氨酸含量逐渐下降(CPM值为378.8±6.4,292.8±14.4,130.6±17.6,230.6±19.4),分别是TGF-β1组(5μg/L)的88.7%,68.6%,30.6%,54.0%.差异均具有显著性(P<0.05).免疫细胞化学结果显示,TGF-β1组(5μg/L)细胞内Ⅰ型与Ⅲ型胶原蛋白平均吸度值分别是对照组的1.36倍和2.12倍,差异具有显著性(P<0.05); Western blotting法结果显示,TGF-β1组的Ⅰ型与Ⅲ型胶原蛋白表达条带吸光度值分别是对照组的1.09倍和1.29倍,差异具有显著性(P<0.05).当给予AcSDKP(10-9mol/L)进行干预时,免疫细胞化学结果显示,细胞内Ⅰ型与Ⅲ型胶原蛋白表达强度较TGF-β1组减弱,其平均吸光度值分别是TGF-β1组的61.3%和68.5%,差异具有显著性(P<0.05).Western blotting法结果显示,Ⅰ型与Ⅲ型胶原蛋白表达条带吸光度值分别是TGF-β1组的83%和54%,差异具有显著性(P<0.05).结论 AcSDKP对TGF-β1介导的心脏成纤维细胞胶原合成与Ⅰ、Ⅲ型胶原蛋白的表达有明显抑制作用,这可能与其抗心脏纤维化的作用相关. 相似文献