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神经营养因子-3基因修饰的嗅鞘细胞移植治疗自身免疫性脑脊髓炎的实验研究 总被引:1,自引:0,他引:1
目的 研究神经营养因子-3(NT-3)基因修饰的嗅鞘细胞移植对自身免疫性脑脊髓炎(EAE)的髓鞘及轴突的修复作用.方法 用逆转录病毒介导神经营养因子-3(NT-3)基因转染嗅鞘细胞(OEC),将其移植入EAE侧脑室,荧光标记后观察其在体内的迁徙、分布特点;运用皮质体感诱发电位监测(CSEP)、超微结构观察、功能评分;RT-PCR方法检测NT-3mRNA转录水平,并与对照组、OEC移植组相比较等方法对髓鞘及轴突修复进行评价.结果 (1)OEC-NT-3在EAE体内存活,可广泛迁徙至病灶远端且至少可以持续存活4周.(2)超微结构发现转基因治疗组4周后,轴索结构完整,周围髓鞘板层结构清楚,明显优于另外二组.(3)4周后,转基因组CSEP潜伏期明显缩短,波幅增加,明显优于其他二组,差异有统计学意义(P<0.05).(4)转基因组NT-3mRNA转录水平为(212.32±16.14)×10-2,明显高于OEC组(1.23±0.13)×10-2及对照组(1.98±0.19)×10-2,差异有统计学意义(P<0.01).结论 NT-3基因修饰的OEC在持续表达神经营养因子,且可促进EAE的神经修复. 相似文献
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局部亚低温治疗对急性脑出血患者应激激素的影响 总被引:5,自引:1,他引:5
目的观察局部亚低温治疗对急性脑出血患者应激激素的影响。方法将78例脑出血患者随机分为亚低温组(38例)和常规治疗组(40例),两组在常规药物综合治疗基础上,亚低温组加用局部亚低温治疗;观察两组治疗前、治疗后第3d、第7d血浆促肾上腺皮质激素(ACTH)、促肾上腺皮质释放激素(CRH)、皮质醇(CS)和醛固酮(ALD)的变化,并与健康对照组比较;比较各组治疗前、治疗第21d两组临床神经功能缺损程度评分(NDS)及疗效。结果两组患者治疗前ACTH、CRH、CS和ALD水平与健康对照组比较明显增高(均P<0.01);治疗第3d、第7d逐步下降,与常规治疗组相比亚低温组各项指标下降更明显(均P<0.01)。治疗第21d亚低温组NDS评分明显低于常规治疗组(P<0.05),显效率、总有效率明显高于常规治疗组(均P<0.01)。结论局部亚低温治疗可降低脑出血患者应激激素分泌,促进患者神经功能恢复,明显改善预后。 相似文献
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BACKGROUND: Olfactory ensheathing cell transplantation can activate axonal regeneration and enhance myelin repair, which are beneficial for treating demyelinating diseases. OBJECTIVE: To explore the effects of olfactory ensheathing cell transplantation on myelin repair, synaptophysin expression, and motor function in a rat model of experimental allergic encephalomyelitis. DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at the Laboratory of Provincial Hospital affiliated to Shandong University between August 2006 and September 2007. MATERIALS: Dibenzylamine (Hoechst 33342), luxol fast blue, and rabbit anti-rat synaptophysin antibody were provided by Sigma, USA. METHODS: Olfactory ensheatbing cells extracted from neonatal Wistar rats were cultured for 10-14 days and labeled with dibenzylamine. Spinal cord extracted from a healthy guinea pig was homogenized and equally mixed with complete Freund's adjuvant; thereafter, the mixture was intracutaneously injected into two posterior voix pedis of healthy male Wistar rats to establish models of experimental allergic encephalomyelitis. Rats were randomly divided into a control encephalomyelitis group and an olfactory ensheathing cell transplantation group, 36 rats in each group. Physiological saline (2 μ L) or an olfactory ensheathing cell suspension (2 μ L) was separately injected along lateral cerebral ventricle at day 7 post-model induction. MAIN OUTCOME MEASURES: The migration and distribution of olfactory ensheathing cells were observed under fluorescence microscopy; myelin repair was detected using hematoxylin-eosin staining and luxol fast blue staining; synaptophysin expression was measured using immunohistochemical staining; motor function was evaluated using a motor function scale. RESULTS: Olfactory ensheatbing cells could survive in vivo and migrate to the distal end of the transplant focus and spinal cord, and survived 21 days. Hematoxylin-eosin staining and luxol fast blue staining indicated that myelin in the transplantation group was intact, and the inflammatory focus gradually disappeared. Transplantation increased synaptophysin expression (P 〈 0.05 versus control) and motor function (P 〈 0.05). CONCLUSION: Olfactory ensheathing cell transplantation can promote myelin repair, increase synaptophysin protein expression, and ameliorate motor function in a rat model of experimental allergic encephalomyelitis. 相似文献
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脑缺血再灌注后不同脑区TNF—α和IL—1β动态变化 总被引:2,自引:0,他引:2
1 材料和方法 : 雄性沙鼠 36只 ,体重 5 0~ 80 g ,随机分假手术组、缺血组。采用常规前脑缺血再灌注模型 ,用10 g/L戊巴比妥钠按每公斤体重 30mg进行麻醉 ,颈正中切口 ,分离双侧颈总动脉并用血管夹夹闭 30min时恢复血流。其中缺血组在术中采用电热垫保持颞肌温度 36~37℃ (半导体测温仪 ) ,分别于再灌注 1、3、6、12、2 4h断头(各组n =6 )。假手术组除不夹闭血管外 ,全过程同缺血对照组。分离纹状体、海马、下丘脑按湿重 10 0mg/mL生理盐水比例用电动匀浆器研磨成匀浆 ,30 0 0r/min离心15min ,取上清液 - 70℃保存… 相似文献
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急性脑梗死患者血小板表达血小板内皮细胞黏附分子-1、P选择素的改变及其意义 总被引:8,自引:4,他引:8
目的观察急性脑梗死(AC I)患者血小板表达血小板内皮细胞黏附分子-1(CD31)、P选择素(CD62p)的改变及其意义。方法采用全血流式细胞术测定53例AC I患者发病48 h内血小板CD31、CD62p的表达水平,并与有脑梗死易患因素组及健康对照组比较。结果AC I组血小板表达CD31、CD62p[(90.91±15.39)%,(7.00±2.96)%]明显高于易患因素组和健康对照组(均P<0.001);AC I组中合并高血压或糖尿病患者血小板CD62p表达高于无高血压和糖尿病的患者(均P<0.01);血小板CD31、CD62p的表达与脑梗死体积正相关(r=0.39,P<0.05;r=0.63,P<0.01)。结论AC I发病后血小板表达CD31、CD62p显著增高,其表达程度与脑梗死体积以及是否合并高血压或糖尿病有关。 相似文献
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关于桡动脉畸形分枝,有些文献已作报导,本文报告高位起始掌浅支一例。患者、郑××,男性,16岁,因头痛发烧,于1980年11月25日就诊。体检中,触诊脉搏时,发现右侧两极并行动脉搏动。脉管距体表很浅,血管搏动明显,强度和搏动频率完全一致,视、触都很清楚,且均呈长脉。两根脉管相距0.5cm。內侧一根搏幅较外侧一根略窄(内侧0.2cm、外侧0.3cm)。以手触摸向近侧追踪发现二者于右桡骨茎突向上方3cm处合而为一。为了证实是桡动脉的分支及其分支位置高低,随用1%亚甲兰1mL于汇合处稍上方约1.5cm处作动脉注射。注射后即发 相似文献