BDNF、PGRN在COPD伴下呼吸道感染中的表达及预后分析

<正>慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)属于慢性气道性炎症疾病,致病因素较多,如吸烟、空气污染、吸入有害化学物质^[1]。COPD损伤气道防御机制,减退清除功能,免疫力下降,病原体入侵导致下呼吸道感染(lower respiratory infection,LRI)^[2]。     

心肌标志物联合检测对血液透析患者并发心血管疾病的诊断价值

目的 探讨心肌标志物联合检测对维持性血液透析(MHD)患者并发心血管疾病的诊断价值。方法 将2020年1月1日至2021年12月31日于该院血液透析中心治疗的患者共205例纳入研究。根据是否并发心血管疾病,将205例患者分为无心血管疾病组(92例)和心血管疾病组(113例)。从临床检查结果中收集患者的检测数据。通过访谈及调查问卷获得患者基本资料。检测纳入研究者血清可溶性生长刺激表达基因2蛋白(sST2)、血清氨基末端脑利钠肽前体(NT-proBNP)、肌钙蛋白T(TNT)、肌红蛋白(MYO)水平。通过超声心动图检测患者左室射血分数(LVEF%)。比较两组患者基本资料和血清标志物水平。对纽约心脏协会(NYHA)分级与各项指标的相关性进行分析。对MHD患者并发心血管疾病的危险因素近分析。采用受试者工作特征(ROC)曲线分析不同心肌标志物对MHD患者并发心血管疾病的诊断效能。采用随机森林法对3种心肌标志物的诊断效能进行比较。采用R语言进行分析,评估不同心肌标志物组合的检测对MHD患者并发心血管疾病诊断的效能。结果 心血管疾病组年龄、糖尿病史比例、开始透析年龄,NT-proBNP、sST2、T...     

Efficacy of poly (lactic-co-glycolic acid) and tetrandrine-loaded nanomicrospheres in the treatment of dry eye北大核心

Objective To discover a nano-drug with anti-inflammatory, sustained-release, and biocompatible properties aimed at blocking the dry eye formation pathway. Methods Nano-microspheres (Tet-ATS@PLGA) composed of tetrandrine (Tet) and poly (lactic-co-glycolic acid) (PLGA) were prepared using the thin-film hydration method, and their stability at room temperature (25 ℃), encapsulation efficiency, and drug loading were tested. Normal rabbit eyes without intervention were recruited in the normal group, and dry eye models were randomly divided into the control group (without any intervention), ATS group (intervened by artificial tears), Tet-ATS group (intervened by artificial tears and Tet), and Tet-ATS@PLGA group (intervened by Tet-ATS@PLGA). Flow cytometry was performed to detect the apoptosis of inflammatory corneal epithelial cells in each group after 24 hours of intervention. The staining of corneal epithelial cells, tear film break-up time (BUT), and surface tear secretion (detected by the Schirmer test strip) were recorded after 14 days of intervention. The thickness of corneal epithelial cells as well as the shape and number of bulbar conjunctival goblet cells were monitored by hematoxylineosin staining. Corneal proteins were extracted for the enzyme-linked immunosorbent assay to measure the expression levels of vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and tumor necrosis factor-α (TNF-α). The independent samples t-test was carried out for comparison among groups. Results The encapsulation efficiency and drug loading of Tet-ATS@PLGA nano-drug were 77.43% and 30.26%, respectively. The drug was stable at room temperature and easy to release when the ocular surface temperature stood at 33 ℃. Compared with other groups, BUT and the amount of tear secretion in the Tet-ATS@PLGA group were the largest, the thickness of corneal epithelial cells was close to the normal value, bulbar conjunctival goblet cells recovered the most, the apoptosis of inflammatory corneal epithelial cells after 24 hours of intervention was the most significant, and the expression levels of VEGF, IL-1β, TNF-α, and PGE2 were the lowest (all P<0.05). Conclusion Tet-ATS@PLGA nano-drug can effectively act on inflammatory corneal epithelial cells in rabbits, promote apoptosis of inflammatory cells, and block the inflammatory response of dry eyes by inhibiting the expression of VEGF, IL-1β, TNF-α, and PGE2, thus improving tear secretion on the ocular surface. © The Author(s) 2023.