稳定表达EGFR胞外区的NIH3T3细胞株的建立及鉴定

目的构建稳定表达表皮生长因子受体(EGFR)胞外区(EGFRex)的NIH3T3细胞株。方法构建带有EGFRex基因的重组真核表达质粒PLNCX2-EGFRex,经脂质体法转染NIH3T3细胞后,用G418筛选阳性克隆,用免疫组化和蛋白质印迹(Western blot)检测EGFRex蛋白的表达,用EGF-EGFP蛋白检测其活性。结果成功构建了真核表达载体PLNCX2-EGFRex。以其转染NIH3T3细胞后,经免疫组化和Western blot检测到EGFRex的表达。在荧光显微镜下见细胞膜上有绿色荧光。结论人EGFRex在NIH3T3细胞内以其天然活性形式稳定表达,为抗体制备奠定了基础。     

稳定表达EGFRvⅢex的NIH3T3细胞株的建立及其免疫原性分析

目的:建立表皮生长因子突变体Ⅲ胞外区(EGFRvⅡ-Iex)的NIH3T3稳定细胞系并分析其免疫原性。方法:将编码EGFRvⅢex基因的表达质粒pLNCX2-EGFRvⅢex转染NIH3T3细胞后,用G418筛选阳性克隆,得到多个细胞克隆。采用免疫组化和Western blot法鉴定这些细胞克隆。选取高表达EG-FRvⅢex的细胞株(命名为3T3-vⅢex)免疫BALB/c小鼠,制备抗血清。用ELISA、Western blot和免疫荧光分别检测抗血清的效价和特异性。结果:成功地构建了真核表达载体pLNCX2-EGFRvⅢex并获得了稳定高表达EGFRvⅢex的NIH3T3细胞系3T3-vⅢex。用3T3-vⅢex免疫小鼠所获得的抗血清效价为10-5。Western blot和免疫荧光鉴定证明抗血清可以与EGFR-vⅢex特异结合。结论:人EGFRvⅢex可在NIH3T3细胞内获得稳定表达,以其免疫小鼠可以获得高效价、高特异性的抗血清。     

江苏地产白首乌 C21甾体苷对高血脂大鼠血脂调节和肝脏保护作用研究

目地研究江苏地产白首乌 C21甾体苷对实验性高脂血症大鼠的血脂调节作用和肝脏保护作用。方法建立实验性高脂血症大鼠模型,成模大鼠分别给予白首乌 C21甾体苷（20、40 mg·kg －1）、阿托伐他汀钙10 mg·kg －1和1％羧甲基纤维素钠溶液灌胃,每日1次,连续给药2周,同时设正常对照组。实验结束比较体质量变化,计算肝脏系数;检测给药后血清总胆固醇（TC）、总甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）含量,并行大鼠肝脏病理学检查。结果给药1周后,阳性对照组和白首乌 C21甾体苷给药组,能够有效控制高脂血症模型大鼠肝脏系数,降低血清 TC、TG 和LDL-C 的含量,其中白首乌 C21甾体苷高剂量组升高血清 HDL-C 的含量与模型组比较有显著差异,病理学切片显示各用药组能改善肝脏脂肪变性。结论白首乌 C21甾体苷具有良好的调节血脂作用,改善肝脏脂肪变性。     

凝血酶和思密达治疗颅脑伤后上消化道出血疗效观察

应激性溃疡出血是颅脑伤后常见并发症,严重影响预后.传统应用孟氏液、硫糖铝治疗,止血速度慢、效果差.为探索快速高效的局部止血药物,我们应用凝血酶和思密达治疗应激性溃疡出血18例,并进行对比观察.     

miR-21对食管癌细胞增殖、凋亡及PI3K/Akt信号通路的影响

目的:探讨miR-21对食管癌细胞增殖及凋亡影响与分子机制.方法:将食管癌细胞分成miR-21-NC组、miR-21组、LY294002组、LY294002+miR-21组、对照组5组,检测miR-21表达情况及食管癌细胞系中PI3K、Akt与p-Akt表达情况.结果:食管癌细胞系的miR-21相对表达量明显高于正常食...     

安徽省农村老年人受虐待与忽视现状及其影响因素研究

目的 探讨安徽省农村老年人受虐待与忽视的现状及影响因素.方法 在安徽省16个地市41个县,对975名60岁以上老年人入户问卷调查.使用自编的调查问卷调查老年人的受教育程度、婚姻状况、收入状况、管教子女情况、对自己父母的行为方式、日常生活能力、生理功能、慢性病情况和老年人受虐待与忽视情况等.结果 最近1年内,一般躯体虐待、严重躯体虐待、情感虐待、经济虐待、忽视和总虐待与忽视的报告率分别为4.5%、1.5%、26.9%、4.9%、7.2%和29.9%.281名受虐待与忽视对象中,80.4%报告最近1年内受到3次以上虐待与忽视,有34.9%报告受到2种及以上的虐待与忽视.5种虐待与忽视的首位施虐者均为儿媳或女婿(43.2%).日常生活功能明显下降、有慢性疾病是一般躯体虐待的危险因素,非文肓是一般躯体虐待的保护因素;日常生活功能明显下降是严重躯体虐待的危险因素;日常生活功能轻度或明显下降、对自己父母有鲁莽行为是情感虐待的危险因素,对子女管教严格是情感虐待的保护因素;日常生活功能轻度或明显下降、经常责打子女、经常对自己父母有鲁莽行为是经济虐待的危险因素;日常生活功能轻度或明显下降、生理功能明显影响日常生活、对自己父母有鲁莽行为是忽视的危险因素;日常生活功能轻度或明显下降、对自己父母有鲁莽行为是总虐待与忽视的危险因素,对子女管教严格是总虐待与忽视的保护因素.结论 农村老年人受虐待与忽视现状不容乐观,不同虐待与忽视类型的影响因素存在一定差异,应加强对日常生活能力下降老年人的关注.

Abstract：

Objective To investigate the prevalence and influencing factors related to abuse and negligence against the elderly in the rural areas. Methods 975 elderly over 60 years from 41counties in Anhui province were included. All participants completed an anonymous questionnaire including items as: educational background, marital condition, income, child-discipline, rude action to parents, daily activities, physical functions, having chronic illness, abuse and negligence against the elderly, etc. Results In the last year, rates of common physical abuse, serious physical abuse,emotional abuse, financial exploitation, negligence, overall abuse and negligence against the elderly were 4.5%, 1.5%, 26.9%, 4.9%, 7.2%, 29.9% respectively. Among the 281 victims, 80.4% reported that they were suffered more than 3 times of abuse and neglect episodes, and 34.9% reported that they were suffered more than 2 forms of abuse and negligence. The primary sadism was carried out by the daughter-in-law or son-in-law (43.2%) of the elderly. Low activity on daily life and having chronic illness were the risk factors causing common physical abuse while better education was the protective factor to it, Low ability in managing daily activity of living was the risk factor causing serions physical abuse. Less active on daily life and having rude action to parents were the risk factors to emotional abuse, but being strict with their children was the protective factor to emotional abuse. Less active on daily life, often beating their children and having rude action to parents were the risk factors related to financial exploitation. Less active on daily life, having rude action to parents and having bad physical functions were the risk factors causing negligence. Less active on daily life and having rude manner to parents were the risk factors of overall elderly abuse and negligence, but being strict with their children was protective factor to the abuse and negligence against the elderly. Conclusion High prevalence on abuse and negligence against the elderly was seen in the rural areas of China. Different forms on elderly abuse and negligence were affected by different factors that called for more attention to be paid to those elderly with lower ability in managing their daily life.     

慢性酒精中毒致脑萎缩24例报道

慢性酒精中毒致脑萎缩２４例报道安徽省宿县地区第二医院神经内科江秀玲慢性酒精中毒可以引起中枢神经系统的严重损害，病程较长时可致脑萎缩［１］．我院自１９８９年７月以来收治２４例慢性酒精中毒的患者，经ＣＴ扫描证实２３例有脑萎缩，现报道如下：１　临床资料：本...     

病案终末质控存在的问题分析与对策

病案是医务人员记录疾病诊疗过程的件，它客观、完整、连续地记录了病人的病情变化及诊疗过程。一份真实而完整的病案不仅能反映医院医疗质量、管理水平、各项核心制度（如三级医师查房制、交接班制度、首诊负责制、会诊制度、疑难病历讨论制度、查对制度、危重病人抢救制度）的执行落实情况；同时也是伤残鉴定、医疗纠纷、保险理赔的法律依据。为此，各级医院从等级医院评审后都将病案质量监控作为医疗质量管理的重要内容。但长期以来绝大部分医院在实施病案质量监控过程中，都是以终末质量监控为主，考核各级医务人员医疗书书写质量的高低。     

基于解剖学课程特质的任务驱动教学模式的构建和应用

<正>近年来,多种新型教学模式的借鉴和应用,对提高教学质量起到积极作用。但总体而言,传统教学模式在医学院校解剖学课堂上仍然处于统治地位,学生被动接受知识的填鸭式教育现状未有根本[1]性改变。任务驱动教学法是以目标任务为导向,以学生为中心,以教师为指导,利用各种教学资源、情景、协作、交互等要素充分发挥学生的主动性、[2]互动性和创新精神的一种教学方法。目前任务驱动教学法已在高校英语、计算机、法学、营销学、会