脂多糖和甲双吡丙酮诱发形成豚鼠运动性哮喘模型

目的建立一种接近临床特点的运动性哮喘动物模型。方法豚鼠２７只分４组。实验组（Ａ组）用脂多糖（１ｍｇ／ｋｇ）和甲双吡丙酮（５０ｍｇ／ｋｇ）腹腔注射，４天后测定气道阻力和动态肺顺应性基础值，８小时后进行运动试验并复测上述指标。对照组有３组（Ｂ、Ｃ、Ｄ组）；分别为腹腔注射脂多糖和甲双吡丙酮不运动组、腹腔注射生理盐水运动组和腹腔注射生理盐水不运动组。结果实验组豚鼠在运动后肺阻力增高、动态肺顺应性降低，而３个对照组上述指标变化均无统计学意义。结论脂多糖和甲双吡丙酮腹腔注射可诱发形成豚鼠运动性哮喘模型     

糖皮质激素类联合肾上腺素能β激动剂对慢性阻塞性肺疾病急性加重期患者的疗效分析

目的 探讨糖皮质激素类联合肾上腺素能β激动剂对慢性阻塞性肺疾病急性加重期(AECOPD)患者的疗效.方法 选取AECOPD患者100例,依据随机数字表法分为联合用药组和对照组,每组50例.对照组给予常规对症和布地奈德雾化吸入治疗,联合用药组在此基础上加用沙丁胺醇雾化吸入治疗.调查所有患者治疗前后肺功能、血清肿瘤坏死因子-α、白细胞介素-8、C-反应蛋白、不良反应、病情严重程度和生活质量情况.结果 联合用药组治疗后1秒用力呼气量、肺总量、1秒用力呼气量用力肺活量百分比显著高于对照组(P<0.05);联合用药组治疗后血清肿瘤坏死因子-α、白细胞介素-8、C-反应蛋白水平低于对照组(P<0.05),治疗后1个月、3个月慢性阻塞性肺疾病评估测试评分和St George呼吸疾病问卷得分低于对照组,差异有统计学意义(P<0.05);两组治疗期间不良反应发生率、治疗后6个月慢性阻塞性肺疾病评估测试评分和St George呼吸疾病问卷得分差异无统计学意义(P>0.05).结论 糖皮质激素类联合肾上腺素能β激动剂治疗可有改善AECOPD患者的肺功能、炎症状态、病情、生活质量和提高疗效,且具有良好的安全性.     

Effects of endothelin antagonist on the bronchoconstriction elicited by isocapnic hyperpnea in Guinea pigs

Effects of endothelin antagonist on the bronchoconstriction elicited by isocapnic hyperpnea in Guinea pigs@梁永杰@蔡映云...     

白介素6、白介素17与慢性阻塞性肺疾病相关性研究

目的探讨慢性阻塞性肺疾病（COPD）患者血浆白介素6（IL-6）、白介素17（IL-17）水平与疾病发生发展和严重程度的关系。方法以90例COPD患者和114例健康对照组为研究对象,以肺功能检测患者COPD严重程度并分组,用酶联免疫吸附实验方法检测各组对象血浆中IL-6和IL-17浓度。结果 COPD组血浆IL-6、IL-17浓度均高于健康对照组（P〈0.05）。重度COPD组血浆IL-6、IL-17浓度高于轻中度COPD组（P〈0.05）。COPD组血浆IL-6、IL-17浓度与FEV1占预计值%和FEV1/FVC均显著呈负相关（P〈0.01）。结论 COPD组血浆中IL-6、IL-17浓度增高,且与COPD的发生、发展、严重程度密切相关。     

COPD患者血浆中ET-1和EDNRB含量变化及临床意义

目的探讨慢性阻塞性肺疾病（chronic obstructive pulmonary disease,COPD）患者血浆内皮素-1（endothelin-1,NET-1）及内皮素受体B（endothelin receptor B,EDNRB）含量变化与COPD发生的关系;这两项指标之间的相关性以及吸烟是否会影响血浆中ET-1及EDNRB的浓度发生变化。方法采用ELISA法测定血浆ET-1、EDNRB的含量。结果COPD患者急性加重期血浆中ET-1和EDNRB的浓度明显高于健康对照组（P〈0．05）,ET-1与EDNP,B含量经统计学处理呈正相关（r=0．284,P〈0．05）。吸烟与非吸烟组ET-1和EDNRB血浆含量的变化无统计学差异（P〉0．05）。结论ET-1、EDNRB是参与COPD急性期发病过程的重要因素。     

INVESTIGATION OF REGULATORY T CELLS IN PATIENTS WITH NON-SMALL CELL LUNG CANCER

Objective To evaluate the prevalence of CD4 ^＋ CD25^high regulatory T cells （ Treg cells） in the peripheral blood mononuclear cells （PBMC） and tumor-infiltrating lymphocytes （TIL） of patients with non-small cell lung cancer （NSCLC） and to investigate immunosuppression to the progression of cancer. Methods Peripheral blood and tumor tissues were collected from 20 patients with NSCLC at the time of surgery. None of the patients received surgery, radiotherapy, chemotherapy, or other medical interventions before this study. Cancer stages of the patients were Ⅰ-Ⅲ A. Venous blood samples were obtained from 20 health donors. PBMC were isolated from blood samples by differential centrifugation over Ficoll-Hypaque. TILs were isolated from tumors by differential centrifugation over Ficoll-Hypaque and Percoll. Percentage of CD4^＋ CD25^highTr/CD4＋T in PBMC and TIL was assessed by the flow cytometry. Results The percentage of CD4^ ＋ CD25high Tr/ CD4 ^＋T in PBMC [ （4. 87 ± 1.22 ） % ] of NSCLC patients was significantly higher than that in healthy donors [ （ 2.36 ± 0. 72 ） % ] （ P 〈 0.01 ）. The percentage of CD4^＋ CD25^highTr/ CD4^＋ T in PBMC [ （5.40 ± 1.20） % ] of NSCLC patients in stage Ⅱ-Ⅲ A was significantly higher than that in stage Ⅰ [ （3. 87 ± 0. 22 ） % ] （ P 〈 0. 01 ）. The percentage of CD4 ＋ CD25hiShTr/ CD4 ＋ T in TIL[ （ 8. 66 ±0. 76） % ] of NSCLC patients in stage Ⅱ-Ⅲ A was significantly higher than that in stage Ⅰ [ （ 7. 04 ± 0. 80） % ] （ P 〈 0. 01 ）. Conclusion The prevalence of CD4 ^＋ CD25^highTreg cells in PBMC and TIL of NSCLC patients was significantly higher than that in healthy donors. These Treg cells may be preventing appropriate antitumor immune responses. The population of CD4^ ＋ CD25^highTreg cells in PBMC and TILs of NSCLC patients with Ⅱ-Ⅲ A stage was significantly higher than that of NSCLC patients with Ⅰ stage. These Treg cells may facilitate development of tumors.     

Assessment of Respiratory lmpedance During Deep Slow Breathing with lmpulse Oscillometry in Patients with Chronic Obstructive Pulmonary Disease

Breathingexerciseisanimportantapproachforrehabilitationofpatientswithchronicobstructivepul monarydisease(COPD).TraditionalChinesemedicine(TCM)hasahistoryofovertwothousandyearsandChinesetraditionalbreathingexercise(Qigong)isanimportantcomponentofTCM.LotsofinvestigationsaboutthetherapeuticeffectsofbreathingexerciseonpatientswithCOPDhavebeenreportedinChina(1,2).　　Deepslowbreathingexercise(DSBE)isoneofbreathingregulationmethodsinChinesetraditionalbreathingexercise.Toourknowledge,thereh…     

Phosphoramidon和Thiorphan对豚鼠气管上皮细胞内皮素—1代谢的影响

目的 观察内皮素转换酶和主要的内皮素降解酶ＮＥＰ对豚鼠气管上皮细胞培养上清液内上素－１（ＥＴ－１）浓度的影响。方法 无菌条件下取豚鼠气管，并用Ｓｃｒａｐｅｒ机械轻刮下上皮细胞，在５％ＣＯ２ ３７℃ＤＭＥＭ／Ｆ１２培养液中培养，不加或分别按０．１ｍｍｏｌ／Ｌ浓度加入中性肽链内切酶－内皮素转换酶双重抑制剂Ｐｈｏｓｐｈｏｒａｍｉｄｏｎ和中性肽链内切酶抑制剂Ｔｈｉｏｒｐｈａｎ继续培养１０小时。收集上清液，     

Kartagener综合症1例

患者，女，25岁。因反复咳嗽、咳痰半年，加重1周人院。体检：T、P、R．BP正常。全身浅表淋巴结无肿大。双侧呼吸运动对称，双肺叩诊清音，呼吸音粗，右下肺闻及湿罗音。心尖搏动位于右锁骨中线第5肋间，心率90次／分，律齐。腹软，无压痛，双下肢不肿。     

紫草素对肺腺癌A549细胞的生长抑制作用及其机制研究

目的观察紫草素抑制人肺腺癌A549细胞的增殖及其机制。方法用不同浓度紫草素处理A549细胞,CCK-8方法检测紫草素对A549细胞生长增殖的抑制作用;细胞周期检测试剂盒测定细胞周期的变化;Western blot法检测磷酸化Akt蛋白（pAkt）的表达。结果紫草素能够抑制人肺腺癌A549细胞的增殖;诱导A549细胞凋亡;阻滞细胞G1期向S期的发展。pAkt蛋白表达量在紫草素处理48 h后明显减少。结论紫草素可以抑制肺腺癌A549细胞的增殖,诱导凋亡,机制可能与紫草素抑制PI3K/Akt信号途径有关。