酶联免疫吸附法测定血小板反应素1及其对前列腺癌诊断价值的研究

目的 采用酶联免疫吸附法(ELISA)测定血小板反应素-1(TSP-1),并分析TSP-1对前列腺癌的诊断价值.方法 用ELISA斑点法测定TSP-1,研制了3种具有分离特异抗原功能的试剂,研发了一项具有纯化蛋白能力的技术,解决非特异蛋白的交叉反应问题,建立测定TSP-1的ELISA常规方法.并用该方法测定14例良性前...     

酶联免疫吸附法测定血小板反应素1及其对前列腺癌诊断价值的研究

Objective To use enzyme-linked immunosorbent assay (ELISA) for measuring thrombospondin-1 (TSP-1),and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non- idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia (BPH) and 18 patients with prostatic carcinoma.Results The TSP-1 values were (73.77±12.72)% and (121.86±-19.47)% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively (t= 8.44,P＜0.01).The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively (P＜0.01).The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.9663 and 0.7421 (P＜0.05).Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.     

北京市首都机场地区支原体感染状况及药敏分析

目的：了解本地区泌尿生殖道支原体感染状况和药敏变化。方法：应用生物？梅里埃支原体IST试剂盒,对泌尿生殖道有临床症状疑患支原体感染的患者采取标本进行支原体培养和药敏试验,并对检验结果进行分析。结果：356例受检患者,其中支原体培养阳性171株（份）（47.9%）。其中解脲支原体（Uu）阳性121株（份）33.9%（121/356）,人型支原体（Mh）阳性29株（份）8.1%（29/356）,Uu＋Mh混合阳性21株（份）5.9%（21/356）。支原体对药物敏感率较高的药物依次是强力霉素（96.4%）、交沙霉素（94.2%）原始霉素90.1%）;耐药较高的是环丙沙星（CIP）26.9%、氧氟沙星（OFL）35.7%。结论：对疑患支原体感染的患者应采取标本进行支原体和药敏检测,参考实验结果有目的规范地选择药物治疗。     

酶联免疫吸附法测定血小板反应素1及其对前列腺癌诊断价值的研究

Objective To use enzyme-linked immunosorbent assay (ELISA) for measuring thrombospondin-1 (TSP-1),and to analyze its diagnostic value for prostatic carcinoma.Methods The possible difficulties and the way to solve the difficulties with ELISA spot were explored first.Three agents which could segregate idio-antigen and one technique which could depurate proteinum were designed and developed.The non- idio- proteinum cross reaction problems were solved and the routine method to measure TSP-1 with ELISA was set up successfully.The serum TSP-1 was measured in 14 patients with benign prostatic hyperplasia (BPH) and 18 patients with prostatic carcinoma.Results The TSP-1 values were (73.77±12.72)% and (121.86±-19.47)% in prostatic carcinoma group and benign prostatic hyperplasia group,respectively (t= 8.44,P＜0.01).The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostatic cancer were 92.7%,88.9% and 85.7%,66.7%,respectively (P＜0.01).The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.9663 and 0.7421 (P＜0.05).Conclusions The determination of TSP-1 with ELISA is feasible.TSP-1 is an ideal diagnostic parameter for prostatic carcinoma and it may distinguish BPH from malignant prostatic disease more exactly than PSA.     

302株铜绿假单胞菌临床分布及耐药分析

铜绿假单胞菌(PAE)是一种氧化酶阳性的非发酵革兰阴性杆菌,连续三年居我院病房病原菌疾病分离率第一位.由于其对多种抗菌药物产生耐药性,使得临床抗感染治疗面临严峻挑战.为探讨铜绿假单胞菌院内感染的耐药情况,对我院三年来分离的302株铜绿假单胞菌的耐药性进行分析,以期为临床有效治疗和控制感染提供参考依据.