48例老年人急性颅内高压的液体疗法

迄今国内外学者对老年急性颅内压增高的液体疗法仍然存在着两种不同的观点 ,一种是严格地限制每日入液量少于2 0 0 0ml/d〔1〕,另一种认为不应限制入液量 ,过分限制入液量有害无益〔2～ 3〕。我们对 5 0例老年急性颅内压增高患者液体疗法进行了研究 ,采取“边补边脱”治疗原则 ,取得较好效果。1　临床资料1 1　一般资料　纳入 1998～ 2 0 0 1年老年急性颅内压增高者48例 ,男 3 2例 ,女 16例 ;60～ 70岁 3 9例 ,70～ 80岁 7例 ,80岁以上 2例 ;外伤性颅内血肿 12例 ,高血压脑出血 3 2例 ,自发性蛛网膜下腔出血 4例。1 2 　诊断标准　临床诊断…     

用于大容量人源天然噬菌体抗体库的表达载体的构建及鉴定

目的构建一个应用于大容量Fab段天然噬菌体抗体库的表达载体。方法用定点突变技术将表面呈现噬菌粒载体pDF上的BssHⅡ酶切位点改为BglⅡ酶切位点,然后分别在抗体轻链和重链位置插入自杀基因SacB,构建含自杀基因的噬菌粒载体pDF-D-SacB;利用抗乙肝表面抗原抗体的基因为模板,PCR扩增重链和轻链基因片段。将PCR扩增的轻链基因和重链基因分别插入载体pDF-D-SacB内,利用电转化的方法将其转入Trans1-Blue大肠杆菌,构建2个初级质粒,再利用初级质粒超感染BSl365菌,使其轻链与重链发生重组,获得重组质粒,进一步获得抗乙肝表面抗原抗体的噬菌体。之后利用该噬菌体感染大肠杆菌Trans1-Blue进行扩增,得到大量抗乙肝表面抗原的噬菌体抗体。最后,通过酶联免疫吸附剂测定检测所获得的抗体。结果通过向pDF重轻链区域插入SacB基因,改造抗体基因克隆位点,构建了pDF-D-SacB载体;经检测,pDF-D-SacB可以表达具有功能的Fab噬菌体抗体,可以在分泌Cre蛋白酶的细菌胞内发生预期的Cre-Loxp介导的定点重组。结论所获得的含自杀基因的噬菌粒载体pDF-D-SacB适用于构建大容量噬菌体抗体库。     

基于核酶自剪切机制稳定分泌丙型肝炎病毒的细胞模型

Objective To construct a stable HCV-producing cell model for anti-HCV drug research. Methods The HCV-ribozyme recombinant plasmid pJFHl-Rbz was constructed to generate the exact 5' and 3' ends of HCV genomic RNA by placing two self-cleaving ribozymes at both ends of the HCV JFH-1 cDNA. The plasmid was then transfected into HepG2 cells and the resultant clones were screened with G418. Subsequently, immunofluorescence and Western blot were performed to detect the expression of HCV core protein, HCV RNA level was quantitated by TaqMan real-time PCR method and HCV particles was detected by electron microscopy. Results HCV core protein was detected in die screened cell clone, and the level of HCV RNA was up to 1 ×107 in the culture medium. Electron microscopy showed the viral particles in the culture suspension were approximately 55 nm in diameter. IFN-treating experiment demonstrated that the HCV RNA level decreased with the increasing concentration of IFN α. Conclusions We constructed a stable HCV-producing cell model which can be used for anti-HCV drug research.     

电子消毒灭菌器应用效果考察

本文应用JR88—MQ—1型电子消毒灭菌器(臭氧发生器)进行臭氧对制剂工作间及无菌工作衣的消毒实验研究。结果证明,当开机60min 后,制剂工作间内达到了无菌状态;开机60min 后,无菌工作衣也基本上达到了无菌状态。同时表明臭氧消毒效果优于人工紫外杀菌灯的消毒,尤其在具有阻挡物体的工作间内,效果更为显着。     

浅析医院药房管理现状及其对策

本文通过对目前国内医院药房管理现状的特征及其存在的主要问题的分析讨论,提出了医院药房必须实行目标管理等对策构思,以期从整体上提高医院药房的管理水平。     

医院制剂洁净室管理模式探讨

本文在医院制剂洁净室管理中，如何保证相应区域的洁净度，从人、物、环境综合管理出发，阐述 了生产环境，生产人员，生产工艺及卫生质量监督四方面应包含的管理内容，使医院制剂洁净室管理从整体上制 度化，规范化，以提高制剂质量，保证用药安全有效。     

基于核酶自剪切机制稳定分泌丙型肝炎病毒的细胞模型

Objective To construct a stable HCV-producing cell model for anti-HCV drug research. Methods The HCV-ribozyme recombinant plasmid pJFHl-Rbz was constructed to generate the exact 5' and 3' ends of HCV genomic RNA by placing two self-cleaving ribozymes at both ends of the HCV JFH-1 cDNA. The plasmid was then transfected into HepG2 cells and the resultant clones were screened with G418. Subsequently, immunofluorescence and Western blot were performed to detect the expression of HCV core protein, HCV RNA level was quantitated by TaqMan real-time PCR method and HCV particles was detected by electron microscopy. Results HCV core protein was detected in die screened cell clone, and the level of HCV RNA was up to 1 ×107 in the culture medium. Electron microscopy showed the viral particles in the culture suspension were approximately 55 nm in diameter. IFN-treating experiment demonstrated that the HCV RNA level decreased with the increasing concentration of IFN α. Conclusions We constructed a stable HCV-producing cell model which can be used for anti-HCV drug research.     

乙型肝炎病毒聚合酶片段的表达及其在血清学检测中的应用

目的建立乙型肝炎患者血清HBV病毒聚合酶抗体(抗-HBp)的ELISA检测方法。方法构建HBV反转录酶/DNA聚合酶(HBV-Pol)基因原核表达载体pQE30-Pol,表达并纯化目的蛋白,用间接ELISA法检测274例乙型肝炎患者及50例正常人血清中的抗-HBp抗体,并与HBV-DNA实时荧光定量检测结果比较。结果成功将HBV-Pol基因插入原核表达载体pQE30,并在大肠埃希菌M15中获得了高效表达,表达产物以包涵体形式存在。以纯化的重组蛋白建立间接ELISA检测方法,在检测的274例乙型肝炎患者中,HBsAg+/HBeAg+/抗HBc+,HBsAg+/抗HBe+/抗HBc+,HBsAg+/抗HBc+患者血清中的抗-HBp抗体阳性率分别为97.8%、57.9%和13.2%,平均阳性率58.8%,而正常人血清抗-HBp均为阴性。运用此法测得的血清抗-HBp阳性率和实时定量PCR检测结果无统计学差异(P=0.359)。结论成功表达了HBV-Pol片段并且建立了血清抗-HBp抗体的检测方法,为进一步研究乙型肝炎患者血清HBV-Pol抗体的血清学意义奠定了基础。     

小脑桥脑角胆脂瘤误诊为原发性三叉神经痛1例

患者女，67岁，因间歇性左侧上下颌部皮肤闪电样疼痛2年，刷牙、洗脸常可诱导疼痛发生，近2月来在家自服卡马西平无效，要求手术来院。查体：神志清，双侧瞳孔等大等圆，光反射灵敏。眼底：双侧视乳头边界清晰。双耳听力正常，鼻唇沟对称，左侧上下颌部皮肤粗糙．痛觉减退。四肢肌力正常。CT及MRI平扫双侧小脑桥脑角及脑部均未见异常。本患。