聚合酶链反应诊断女性外阴尖锐湿疣初步探讨

聚合酶链反应诊断女性外阴尖锐湿疣初步探讨郑惠方单祥年李明发蒋清龚嘉仪（南京铁道医学院附属医院妇产科，生物学教研室，附属医院病理科南京２１０００９）尖锐湿疣（ＣＡ）主要由人乳头瘤病毒（ＨＰＶ）６及１１型ＤＮＡ感染引起。尖锐湿疣和假性湿疣...     

Flow-induced Expression and Phosphorylation of VASPin Endothelial Cells

1 Introduction It is well known that mechanical forces have important influence on endothelial cells, in particular, on cytoskeleton reorganization. VASP (vasodilator stimulated phosphoprotein) is a 46 KD actin associated protein. It is a member of Ena/VASP protein family and composed of EVH1, proline-rich and EVH2 domains. It is considered as an important component of the sub-cellular regions where remodelling of the actin cytoskeleton takes place, such as the front of spreading lamell…     

Chronic neuroprotective effects of low concentration lithium on SH-SY5Y cells:possible involvement of stress proteins and gene expression

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate(Li2CO2) for 25–50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2(PKM2) and calmodulin 3(CaM 3) expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II(CK2), threonine/tyrosine phosphatase 7(PYST2), and dopamine beta-hydroxylase(DBH) expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins(HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins: a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.     

百令胶囊对频繁发作 ＣＯＰＤ 病人免疫功能和肺功能的影响

目的：观察百令胶囊对频繁发作慢性阻塞性肺疾病（ＣＯＰＤ）病人免疫功能和肺功能的影响。方法： 选取 １００ 例年发作次数在 ２ 次以上，每次发作均来本院呼吸内科住院诊治的慢性阻塞性肺疾病急性加重期病人 （ＡＥＣＯＰＤ）。５２ 例对照组病人在常规治疗的基础上增加无创正压通气（ＮＩＰＰＶ），４８ 例观察组病人在对照组之 上增加百令胶囊治疗。观察两组病人治疗前后免疫功能和肺功能情况。结果：观察组临床治疗有效率为８３．３３％ 显著高于对照组的６５．３８％，有统计学意义（χ２ ＝ ４．１８，Ｐ＜０．０５）；观察组治疗后 ＦＥＶ１ 、ＦＥＶ１ 预测值、ＦＥＶ１ ／ ＦＶＣ 均 高于对照组，差异有统计学意义（Ｐ＜０．０５）；观察组治疗后 Ｔ 淋巴细胞亚群 ＣＤ４＋、ＣＤ４＋ ／ ＣＤ８＋高于对照组，ＣＤ８ ＋低于对照组，均有统计学差异（Ｐ＜０．０５）。结论：采用百令胶囊辅助治疗，可改善病人免疫功能，提高肺功能，有 望降低 ＡＥＣＯＰＤ 发作次数。     

手足口病儿童血浆γ干扰素诱导蛋白10的表达水平及临床意义

目的:探讨γ干扰素诱导蛋白10(interferon-γ inducible protein-10,IP-10)在手足口病(hand-foot-mouth disease,HFMD)儿童血浆中的表达水平及临床意义?方法:选取HFMD患儿74例,根据是否伴有神经系统症状将患儿分为重症组和普通感染组;另选取30例体检儿童作为健康对照组?采用流式液相多重蛋白定量检测技术检测血浆中IP-10的表达量,同时检测分析淋巴细胞亚群比例?免疫球蛋白和血常规相关指标?比较各指标在健康对照组?HFMD普通感染组及重症组中的差异;分析IP-10与皮疹出现天数及淋巴细胞各亚群比例之间的相关性?结果:HFMD普通感染组及重症组患儿白细胞计数均增高,但和健康对照组相比无统计学差异?HFMD普通感染组及重症组患儿IP-10蛋白表达均显著增加(P < 0.001),重症组IP-10蛋白表达略低于普通感染组,差异无统计学意义(P > 0.05)?HFMD普通感染组及重症组患儿T细胞比例显著降低(P < 0.001),其中CD4+ T 淋巴细胞与CD8+ T 淋巴细胞亦显著降低(P < 0.05)?相反,B淋巴细胞比例显著增加(P < 0.001)?HFMD普通感染组?重症组与健康对照组相比,IgA降低,IgG增加,但无统计学差异(P > 0.05)?HFMD普通感染组患儿IP-10蛋白表达量与皮疹天数呈负相关(P < 0.05),而重症组虽IP-10蛋白表达量与皮疹天数呈负相关,但无统计学意义?HFMD重症组IP-10蛋白表达量与T淋巴细胞比例呈正相关,其中与CD4+ T 淋巴细胞的正相关有统计学意义(P < 0.01)?结论:HFMD患儿T淋巴细胞比例降低,B淋巴细胞比例增加,IP-10蛋白表达显著增加,与皮疹天数呈负相关?     

正确和完整解读《甲乙经》的学术思想——《甲乙经》析疑五则

《甲乙经》一书,乃《针灸甲乙经》之简称,为晋代皇甫谧据《素问》、《针经》、《针灸治要》三书内容除去重复重新归类编撰而成,是针灸学的一部宝贵典籍。由于年代久远,文字错简脱落者有之,亥豕鲁鱼者有之,字形义训变迁者亦有之,今人一般很难通读之,必须采用研究古籍方法始克奏效。     

从肝论治疑难病案分析

患者张某,男,44岁,1980年5月22日门诊。 据患者述1960年感右胁隐痛,疑为肝炎,而作超声波、肝功能检查,结果均无异常发现。右胁隐痛持续至1965年5月,每日痛2～3次,有时可间歇半个月不痛,痛位不甚固定,并连及右腰背,当时又疑为胆囊炎,在某院住院检查,行胆囊造影及。肾脏造影均无异常。     

Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?

AIM:To analyze the role of rs12979860 and rs8099917polymorphisms in hepatitis C virus(HCV)genotype 1infection of Brazilians.METHODS:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C(CHC)who had completed a 48-wk regimen of pegylated-interferonα-2a or-2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and199 healthy blood donors(controls)from a single site between January 2010 and January 2012.Data on the patients’response to treatment was collected.Polymerase chain reaction-restriction fragment length polymorphism genotyping of the interleukin(IL)28B gene fragment encompassing the single nucleotide polymorphisms(SNPs)rs12979860(C/T)and rs8099917(T/G)was carried out for 79 of the CHC patients and 199 of the controls.Bi-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC patients.RESULTS:SNP rs12979860 genotyping was successful in 99.5%of the controls and 97.2%of the CHC patients,whereas the SNP rs8099917 genotyping was successful in 95.5%of the controls and 100%of the CHC patients.The genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups,with significantly higher genotype frequencies of CC and TT in the controls(P=0.037 and 0.046,respectively)and of TT and GG in the CHC patients(P=0.0009and 0.0001,respectively).Analysis of the CHC patients who achieved sustained virological response(SVR)to treatment(n=55)indicated that the rs12979860 C allele and CC genotype were predictors of SVR(P=0.02).No significant correlation was found between rs8099917 genotypes and treatment response,but carriers of the T allele showed significantly higher rates of SVR(P=0.02).Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917(P=0.07).CONCLUSION:The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCVinfected individuals may indicate a potential pro     

周围神经卡压综合征影像诊断的研究进展

周围神经经过某些解剖上狭窄部位的急、慢性挤压或者反复摩擦造成局部水肿等炎症反应,使局部容积减小,血液循环障碍,造成该处甚至远处神经发生脱髓鞘改变,出现支配区不同程度的运动及感觉功能障碍的现象称之为神经卡压综合征。传统的周围神经卡压综合征的诊断主要依靠其临床表现、物理检查及电生理检测,是一种主观的判断。近年     

肝外伤112例诊治体会

肝外伤是腹部外科中死亡率较高的脏器伤,需及时、正确的诊断和处理。我院自1996年1月至2009年3月共收治肝外伤患者112例,其中男98例,女14例,年龄8～64岁,平均36．5岁。伤后至入院时间1～6h,平均4．5h。损伤原因：交通事故79例,坠落伤14例,钝击伤8例,刀刺伤10例,枪击伤1例。闭合性损伤97例,开放性损伤15例。