排序方式: 共有13条查询结果,搜索用时 15 毫秒
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Takuto Chiba Dbora M. Cerqueira Yao Li Andrew J. Bodnar Elina Mukherjee Katherine Pfister Yu Leng Phua Kai Shaikh Brandon T. Sanders Shelby L. Hemker Patrick J. Pagano Yijen L. Wu Jacqueline Ho Sunder Sims-Lucas 《Journal of the American Society of Nephrology : JASN》2021,32(3):553
BackgroundDamage to the renal microvasculature is a hallmark of renal ischemia-reperfusion injury (IRI)–mediated AKI. The miR-17∼92 miRNA cluster (encoding miR-17, -18a, -19a, -20a, -19b-1, and -92a-1) regulates angiogenesis in multiple settings, but no definitive role in renal endothelium during AKI pathogenesis has been established.MethodsAntibodies bound to magnetic beads were utilized to selectively enrich for renal endothelial cells from mice. Endothelial-specific miR-17∼92 knockout (miR-17∼92endo−/−) mice were generated and given renal IRI. Mice were monitored for the development of AKI using serum chemistries and histology and for renal blood flow using magnetic resonance imaging (MRI) and laser Doppler imaging. Mice were treated with miRNA mimics during renal IRI, and therapeutic efficacies were evaluated.ResultsmiR-17, -18a, -20a, -19b, and pri–miR-17∼92 are dynamically regulated in renal endothelial cells after renal IRI. miR-17∼92endo−/− exacerbates renal IRI in male and female mice. Specifically, miR-17∼92endo−/− promotes renal tubular injury, reduces renal blood flow, promotes microvascular rarefaction, increases renal oxidative stress, and promotes macrophage infiltration to injured kidneys. The potent antiangiogenic factor thrombospondin 1 (TSP1) is highly expressed in renal endothelium in miR-17∼92endo−/− after renal IRI and is a target of miR-18a and miR-19a/b. miR-17∼92 is critical in the angiogenic response after renal IRI, which treatment with miR-18a and miR-19b mimics can mitigate.ConclusionsThese data suggest that endothelial-derived miR-17∼92 stimulates a reparative response in damaged renal vasculature during renal IRI by regulating angiogenic pathways. 相似文献
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Yijen Wu Patrice S Pearce Amedeo Rapuano T Kevin Hitchens Nihal C de Lanerolle Jullie W Pan 《Journal of cerebral blood flow and metabolism》2015,35(11):1862-1870
There is little experimental in vivo data on how differences in seizure duration in experimental status epilepticus influence metabolic injury. This is of interest given that in humans, status duration is a factor that influences the probability of subsequent development of epilepsy. This question is studied using 7-T magnetic resonance (MR) spectroscopy, T2 relaxometry in the incremented kainate rodent model of temporal lobe epilepsy, using two durations of status epilepticus, 1.5 and 3 hours. Histologic evaluation was performed in a subset of animals. Three days after status, single-voxel (8 mm3) point resolved spectroscopy (PRESS) MR spectroscopic measurements were acquired at 7 T to assess the cerebral metabolites measured as a ratio to total creatine (tCr). The status injury resulted in decreased N-acetylaspartate NAA/tCr, increased myo-inositol/tCr and glutamine/tCr, increased T2, and significant declines in NeuN-stained neuronal counts in both status groups. Regressions were identified in the status groups that provide evidence for neuronal injury and astrocytic reaction after status in both the short and long status duration groups. The long status group displays changes in glutathione/tCr that are not identified in the short status group, this difference possibly representing a maturation of injury and antioxidant response that occurs in synchrony with glutamatergic injury and glial activation. 相似文献
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Chitinase-3-like 1 protein complexes modulate macrophage-mediated immune suppression in glioblastoma
Apeng Chen Yinan Jiang Zhengwei Li Lingxiang Wu Ulises Santiago Han Zou Chunhui Cai Vaibhav Sharma Yongchang Guan Lauren H. McCarl Jie Ma Yijen L. Wu Joshua Michel Yi Shi Liza Konnikova Nduka M. Amankulor Pascal O. Zinn Gary Kohanbash Sameer Agnihotri Songjian Lu Xinghua Lu Dandan Sun George K. Gittes Qianghu Wang Xiangwei Xiao Dean Yimlamai Ian F. Pollack Carlos J. Camacho Baoli Hu 《The Journal of clinical investigation》2021,131(16)
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Yijen A. Huang Yutaka Maruyama Robert Stimac Stephen D. Roper 《The Journal of physiology》2008,586(12):2903-2912
Taste buds contain two types of cells that directly participate in taste transduction – receptor (Type II) cells and presynaptic (Type III) cells. Receptor cells respond to sweet, bitter and umami taste stimulation but until recently the identity of cells that respond directly to sour (acid) tastants has only been inferred from recordings in situ, from behavioural studies, and from immunostaining for putative sour transduction molecules. Using calcium imaging on single isolated taste cells and with biosensor cells to identify neurotransmitter release, we show that presynaptic (Type III) cells specifically respond to acid taste stimulation and release serotonin. By recording responses in cells isolated from taste buds and in taste cells in lingual slices to acetic acid titrated to different acid levels (pH), we also show that the active stimulus for acid taste is the membrane-permeant, uncharged acetic acid moiety (CH3 COOH), not free protons (H+ ). That observation is consistent with the proximate stimulus for acid taste being intracellular acidification, not extracellular protons per se . These findings may also have implications for other sensory receptors that respond to acids, such as nociceptors. 相似文献
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In situ labeling of immune cells with iron oxide particles: an approach to detect organ rejection by cellular MRI 总被引:5,自引:0,他引:5
Wu YL Ye Q Foley LM Hitchens TK Sato K Williams JB Ho C 《Proceedings of the National Academy of Sciences of the United States of America》2006,103(6):1852-1857
In vivo cell tracking by MRI can provide means to observe biological processes and monitor cell therapy directly. Immune cells, e.g., macrophages, play crucial roles in many pathophysiological processes, including organ rejection, inflammation, autoimmune diseases, cancer, atherosclerotic plaque formation, numerous neurological disorders, etc. The current gold standard for diagnosing and staging rejection after organ transplantation is biopsy, which is not only invasive, but also prone to sampling errors. Here, we report a noninvasive approach using MRI to detect graft rejection after solid organ transplantation. In addition, we present the feasibility of imaging individual macrophages in vivo by MRI in a rodent heterotopic working-heart transplantation model using a more sensitive contrast agent, the micrometer-sized paramagnetic iron oxide particle, as a methodology to detect acute cardiac rejection. 相似文献
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Ling Xiaoxi Latoche Joseph D. Choy Cindy J. Kurland Brenda F. Laymon Charles M. Wu Yijen Salamacha Nathan Shen Ding Geruntho Jonathan J. Rigatti Lora H. Windish Hillarie P. Langton-Webster Beatrice Berkman Clifford E. Anderson Carolyn J. 《Molecular imaging and biology》2020,22(2):274-284
Molecular Imaging and Biology - Prostate-specific membrane antigen (PSMA) continues to be the hallmark biomarker for prostate cancer as it is expressed on nearly all prostatic tumors. In addition,... 相似文献
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Li Liu Qing Ye Yijen Wu Wen-Yuan Hsieh Chih-Lung Chen Hsin-Hsin Shen Shian-Jy Wang Haosen Zhang T. Kevin Hitchens Chien Ho 《Nanomedicine : nanotechnology, biology, and medicine》2012,8(8):1345-1354
Non-invasive in vivo tracking of T-cells by magnetic resonance imaging (MRI) can lead to a better understanding of many pathophysiological situations, including AIDS, cancer, diabetes, graft rejection. However, an efficient MRI contrast agent and a reliable technique to track non-phagocytic T-cells are needed. We report a novel superparamagnetic nano-sized iron-oxide particle, IOPC-NH2 series particles, coated with polyethylene glycol (PEG), with high transverse relaxivity (250 s?1 mM?1), thus useful for MRI studies. IOPC-NH2 particles are the first reported magnetic particles that can label rat and human T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation. IOPC-NH2 particles do not cause any measurable effects on T-cell properties. Infiltration of IOPC-NH2?labeled T-cells can be detected in a rat model of heart-lung transplantation by in vivo MRI. IOPC-NH2 is potentially valuable contrast agents for labeling a variety of cells for basic and clinical cellular MRI studies, e.g., cellular therapy.From the Clinical EditorIn this study, a novel PEG coated superparamagnetic nano-sized iron-oxide particle was investigated as a T-cell labeling agent for MRI studies. The reported particles can label T-cells with over 90% efficiency, without using transfection agents, HIV-1 transactivator peptide, or electroporation, therefore may enable more convenient preclinical call labeling studies. 相似文献