基于知识元标引的中医古籍研究回顾与反思

回顾国内以往知识元研究,结合当前中医古籍知识元研究工作,探讨中医古籍研究的部分现存问题.中医古籍知识元研究工作重点在于知识元标引平台的研发以及中医基础理论的数据化处理.知识元的概念是文本中的词或短语;知识元的文本可以借助文言语法与知识图谱三元组概念进行数据提取;建立语义关系,即语义三元组的描述,可分为表达不同描述对象(...     

Pharmacy Student Self-Testing as a Predictor of Examination Performance

Objectives. To determine if student self-testing improves performance during a doctor of pharmacy course.Methods. Students were given access to online quizzes with a large pool of randomly selected questions specific to upcoming examination content. Quizzes were electronically scored immediately upon completion and students were provided corrective feedback.Results. Examination scores following implementation of the practice quizzes were significantly higher in all but the last testing period. The upper fiftieth percentile of students scored higher on both the practice quizzes and subsequent examinations in all but the fourth testing period.Conclusions. Providing pharmacy students with self-testing opportunities could increase their retention of course material and provide feedback to both students and educators regarding learning, as well as provide students with a measure of their metacognition.     

产妇血清表面抗原定量与乳汁中乙肝病毒载量的相关性分析

目的 探讨携带乙型肝炎病毒(HBV)的产妇血清表面抗原(HBsAg)含量与乳汁中HBV-DNA含量的相关性,并指导母乳喂养.方法 对140例产妇采用荧光定量PCR法测定乳汁中HBV-DNA和化学发光法测定表面抗原含量,并进行相关性分析.结果 A组乳汁HBV-DNA阳性组表面抗原含量为(58 183.2±39 147.8) IU/ml,B组HBV-DNA阴性组表面抗原含量为(1848.8±2458.3) IU/ml.A组血清中表面抗原含量比B组高,差异有统计学意义(P＜0.01),乳汁中HBV-DNA含量与血清中表面抗原含量呈正相关(r=0.799,P＜0.01).结论 慢性HBV感染孕产妇血清中乙肝表面抗原的定量的测定及检测其乳汁中HBV-DNA含量,对阻断母婴传播和预防家庭内水平传播及对HBV感染孕产妇的诊治和疗效评估有着重要意义,特别是为HBV感染产妇选择是否母乳喂养提供了指导依据.     

碘治疗工作场所空气中131I的监测结果与分析

目的 了解碘治疗工作场所空气中¹³¹I气溶胶的活度浓度,估算核医学科医务人员吸入¹³¹I所致内照射剂量。方法 使用CF-1001BRL型便携式大容量空气采样器,采用碘盒收集山东省6家医院核医学科碘治疗工作场所空气中的¹³¹I气溶胶,利用HPGe-γ能谱仪对样品进行测量,得到6家医院碘治疗工作场所中¹³¹I的活度浓度值,并估算医务人员的内照射剂量。结果 6家医院碘治疗工作场所空气中¹³¹I的活度浓度范围为3.64~2.94×10³Bq/m³,控制区（病房、患者通道、分装间、远程操作给药室）¹³¹I的浓度水平明显高于监督区,监督区¹³¹I的浓度最高的是医护通道,为2.62×10²Bq/m³。核医学科医务人员两种职业待积有效剂量估算值为0.07~5.68 mSv,均未超过国家规定限值。结论 医院核医学科碘治疗工作场所仍存在不可忽视的¹³¹I气溶胶污染现象,应面向全国各地区核医学科开展内照射监测,探索更加合理的防护标准和方法。     

Computational study on natural compounds inhibitor of c-Myc

To screen and identify ideal leading compounds from a drug library (ZINC15 database) with potential inhibition effect against c-Myc to contribute to medication design and development.A series of computer-aided virtual screening techniques were performed to identify potential inhibitors of c-Myc. LibDock from the software Discovery Studio was used to do a structure-based screening after ADME (absorption, distribution, metabolism, excretion) and toxicity prediction. Molecular docking was utilized to show the binding affinity and potential mechanism between ligands and c-Myc. Stability of the ligand-receptor complex was analyzed by molecular dynamic simulation at the end of the research.Compounds with more interactive energy which are confirmed to be the potential inhibitors for c-Myc were identified from the ZINC15 databases. Additionally, those compounds are also anticipated with fewer ames mutagenicity, rodent carcinogenicity, nondevelopmental toxic potential, and tolerant with cytochrome p450 2D6(CYP2D6). Dynamic simulation analysis also revealed that the very compounds had more favorable potential energy compared with 10058-F4(ZINC12406714). Furthermore, we prove that those compounds are stable and can exist in natural conditions.This study demonstrates that the compounds are potential therapeutic inhibitors for c-Myc. These compounds are safe and stable for drug candidates and may play a critical role in c-Myc inhibitor development.     

Amphiphilic block copolymers bearing six-membered ortho ester ring in side chains as potential drug carriers: synthesis,characterization, and in vivo toxicity evaluation

A new type of amphiphilic block copolymers, poly(ethylene glycol)-block-poly(2-methyl-acrylicacid 2-methoxy-5-methyl-[1,3]dioxin-5-ylmethyl ester) (PEG-b-PMME), bearing acid-labile six-membered ortho ester rings in side chains was synthesized by reversible addition–fragmentation chain-transfer polymerization, and the influence of chain length of the hydrophobic PMME block on micelle properties was investigated. The PEG-b-PMME micelles were stable in aqueous buffer at physiological pH with a low critical micelle concentration. Nile Red as a model drug was encapsulated into the micelles to explore the release profiles. The Nile Red-loaded polymeric micelles showed rapid release of Nile Red in weakly acidic environments (pH 5) but slow release under physiological condition (pH 7.4), due to different hydrolysis rate of ortho ester side chains of PEG-b-PMME. The Paclitaxel (PTX)-loaded micelles retained potency in killing lung cancer cells (A549), compared with the free PTX. No obvious toxicity was found in vitro and in vivo after intraperitoneal injection of the micelles, which confirms that the PEG-b-PMME micelles with unique acid-labile characteristic have great potential as nano-scaled carriers for drug delivery.     

葛根素注射液致高血压患者尿糖和血糖变化的观察

目的:观察葛根素注射液致高血压患者尿糖和血糖的变化。方法:19例高血压患者[男性11例,女性8例,平均年龄(71.11±3.54)岁]予葛根素500mg溶于250ml0.9%氯化钠注射液中静脉滴注。用药开始后1h、5h、11h和21h(次日空腹)以及次日(停用葛根素)的上述相同时间点检测尿糖和血糖水平。结果:用药开始1h、5h及11h的尿糖阳性率分别为15.8%、84.2%和36.8%。次日相同时间点的阳性率分别为0、5.26%和5.26%。前者的尿糖阳性率显著高于后者,差异有统计学意义(P<0.05,P<0.01,P<0.05)。用药开始后21h尿糖检测呈阴性反应。前者的血糖浓度和后者比较,差异均无统计学意义(P>0.05)。结论:葛根素注射液可致高血压患者出现尿糖阳性,停药后迅速转阴,对血糖无明显影响。