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排序方式: 共有262条查询结果,搜索用时 15 毫秒
1.
TP Amadeu† AB Seabra‡ MG de Oliveira‡ AMA Costa† 《Journal of the European Academy of Dermatology and Venereology》2007,21(5):629-637
BACKGROUND: Nitric oxide (NO) plays a key role in wound repair and S-nitrosothiols like S-nitrosoglutathione (GSNO) are well known NO donors. METHODS: Animals were separated in two groups and submitted to excisional wounds on the dorsal surface at the first day. GSNO (100 microm)-containing hydrogels were topically applied on the wound bed in the GSNO group, daily, during the first 4 days. Control group was topically treated with hydrogel without GSNO for the same period. Wound contraction and re-epithelialization were measured. Animals were sacrificed 21 days after wounding. Samples of lesion and normal tissue were formalin-fixed, paraffin embedded for histological analysis. RESULTS: Wound contraction, measured 14 and 21 days after wounding, was greater in the GSNO group than in the control group (P<0.05 for both). The re-epithelialized wound area, measured 14 days after wounding, was higher in the GSNO group than in the control group (P<0.05). A higher amount of inflammatory cells was observed in superficial and deep areas of the granulation tissue of the control group compared to the GSNO group. Twenty-one days after wounding, thin red-yellow collagen fibers arranged perpendicularly to the surface were found in the granulation tissue of the control group, whereas in the GSNO-treated group collagen fibers were thicker and arranged parallel to the surface. Increased number of mast cells was observed in the GSNO group compared with that in the control group. Vascularization and myofibroblast distribution were similar in both groups. CONCLUSION: Topical application of GSNO-containing hydrogel during the early phases of rat cutaneous wound repair accelerates wound closure and re-epithelialization and affects granulation tissue organization. 相似文献
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A cell-free model of the encephalomyocarditis virus-induced inhibition of host cell protein synthesis. 总被引:9,自引:0,他引:9
When preincubated extracts from Krebs-II cells were supplemented with total poly(A)-containing RNA isolated from encephalomyocarditis virus-infected Krebs-II cells, two phenomena characteristic of EMC virus infection of these cells in vivo were observed: (i) preferential translation of viral rather than cellular, mRNA, and (ii) a general inhibition of protein synthesis, relative to the synthesis in samples where the poly(A)-containing RNA from uninfected cells served as the template. The first effect could not be explained by an irreversible functional inactivation of cellular templates and seemed to result from a direct interference of viral RNA with the translation of cellular mRNA. The second effect was due primarily to the presence of double-stranded RNA. 相似文献
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Stein TP; Oram-Smith JC; Leskiw MJ; Wallace HW; Long LC; Leonard JM 《The American journal of physiology》1976,230(5):1321-1325
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Summary The effect of DNA on the radiosensitivity of a protein included into the nucleoprotein complex was studied. Since biological activity, the change of which may conveniently serve as a criterion of protein disintegration, cannot yet be detected in the protein constituents of natural nucleoproteins, the authors used an artificial DNA--chymotrypsim complex for this investigation. It was found that after x-ray irradiation (10.000 and 50.000 r) of solutions of these complexes and of free -chymotrypsim (control) the free enzyme is much more resistant to the action of the radiation than the same enzyme combined with DNA. The formation of different complexes may possibly be one of the factors which intensifies or reduces the radiosensitivity of the individual biological components of the cell.(Presented by Active Member AMN SSSR N.N. Zhukov-Verezhnikov) Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 50, No. 7, pp. 55–58, July, 1960 相似文献
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Y.V. Svitkin T.Y. Ugarova T.V. Chernovskaya V.N. Lyapustin V.A. Lashkevich V.I. Agol 《Virology》1981,110(1):26-34
RNA isolated from tick-borne encephalitis virus (TBEV) was translated in an mRNA-dependent cell-free system derived from Krebs-2 cells, producing a set of polypeptides with Mr ranging from ca. 13,000 to 160,000 and higher. Two of these polypeptides with Mr of 13,000 (p13) and 53,000 (p53) were identified as TBEV core polypeptide V2 and the polypeptide moiety of envelope glycoprotein, V3, respectively. The amino acid sequences of p13 and p53 were also shown to be contained in a high-molecular-weight polypeptide (Mr ~ 118,000). The label from the initiator tRNA species, f-[35S]Met-tRNAfMet, could be incorporated into p13 but not into p53, suggesting that p13 is encoded in a region of the viral genome immediately adjacent to the site at which the translation in vitro is initiated; on the other hand, the p53 coding segment appears to be located further from the initiation point. The two structural polypeptides of TBEV were accumulated in vitro with dissimilar kinetics and both differed from the majority of other translation products in that their synthesis was relatively resistant to an increase in KCl concentration in the incubation mixture. Possible modes of synthesis of TBEV structural proteins and post-translational processing of their precursor are discussed. 相似文献
8.
双能量X线骨质密度测量仪监测小儿下肢骨延长骨矿物质的变化 总被引:1,自引:0,他引:1
目的 在儿童骨延长的患儿中 ,为了能够有效地控制骨延长的速率 ,达到骨延长的目的 ,采用双能量X线骨质密度测量仪 (dualenergyX Rayabsorptiometry ,DEXA)监测延长断端骨矿含量 (bonemineralcontent,BMC)的变化。方法 30例患儿中有 5 0处下肢作了骨延长术 ,平均年龄10 .9岁 (5~ 17岁 ) ,引起短肢的病因不同。术后 7~ 10d开始行骨延长 ,每次延长 0 .2 5mm ,每天 4次。牵引延长期间每周扫描一次 ,拆除外固定器后每 2周扫描一次到术后 2年。DEXA扫描的分辨率是 1mm× 1mm ,扫描速度 30mm/s。比较不同延长时期中骨矿含量的变化。分析不同病因和不同外固定器之间骨矿含量变化的差别。结果 不同固定器之间骨矿含量的差别无著性意义。根据骨延长区BMC增加速率 ,将患儿分为快速组、一般组和慢速组。快速组每日BMC增加速率为 0 .3%~ 0 .6 % ,新骨生长快速 ;一般组每日BMC增加 0 .1%~ 0 .3% ,新骨中速生长 ;慢速组每日增加 <0 .1% ,新骨生成缓慢。骨矿化速率与原发病因相关。结论 DEXA能动态监测骨延长中新生骨的骨矿含量的变化 ,根据骨矿含量变化的程度 ,能够调整骨延长的速率 ,从而达到预期骨延长的目的。 相似文献
9.
WG Mitchell H Lynn JF Bale MA Maeder SM Donfield B Garg AH Tilton JK Willis TP Bohan 《Pediatrics》1997,100(5):817-824
BACKGROUND: Boys and young men with hemophilia treated with factor infusions before 1985 had a substantial risk of acquiring the human immunodeficiency virus (HIV) and the acquired immunodeficiency syndrome. This study was designed to assess the effects of HIV and hemophilia per se on neurological function in a large cohort of subjects with hemophilia, and to investigate the relationships between neurological disease and death during follow-up. METHODS: Three hundred thirty-three boys and young men (207 HIV seropositive and 126 HIV seronegative) were evaluated longitudinally in a multicenter, multidisciplinary study. Neurological history and examination were conducted at baseline and annually for 4 years. The relationship between neurological variables, HIV serostatus, CD4+ cell counts, and vital status at the conclusion of the study was examined using logistic regression models. RESULTS: The risks of nonhemophilia-associated muscle atrophy, behavior change, and gait disturbance increased with time in immune compromised HIV-seropositive subjects compared with HIV seronegative or immunologically stable HIV-seropositive subjects. The risk of behavior change in immune compromised HIV-seropositive hemophiliacs, for example, rose to 60% by year 4 versus 10% to 17% for the other study groups. Forty-five subjects (13.5%), all of whom were HIV seropositive, died by year 4. Subjects who died had had increased risks of hyperreflexia, nonhemophilia-associated muscle atrophy, and behavior change. CONCLUSIONS: These results indicate that immune compromised, HIV-seropositive hemophiliacs have high rates of neurological abnormalities over time and that neurological abnormalities were common among subjects who later died. By contrast, immunologically stable HIV-seropositive subjects did not differ from the HIV-seronegative participants. Hemophilia per se was associated with progressive abnormalities of gait, coordination, and motor function. 相似文献
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