全文获取类型
收费全文 | 7118篇 |
免费 | 372篇 |
国内免费 | 76篇 |
专业分类
耳鼻咽喉 | 28篇 |
儿科学 | 141篇 |
妇产科学 | 133篇 |
基础医学 | 1079篇 |
口腔科学 | 146篇 |
临床医学 | 426篇 |
内科学 | 1749篇 |
皮肤病学 | 252篇 |
神经病学 | 600篇 |
特种医学 | 250篇 |
外科学 | 1079篇 |
综合类 | 23篇 |
预防医学 | 178篇 |
眼科学 | 73篇 |
药学 | 534篇 |
中国医学 | 18篇 |
肿瘤学 | 857篇 |
出版年
2023年 | 27篇 |
2022年 | 89篇 |
2021年 | 148篇 |
2020年 | 77篇 |
2019年 | 102篇 |
2018年 | 149篇 |
2017年 | 158篇 |
2016年 | 169篇 |
2015年 | 170篇 |
2014年 | 242篇 |
2013年 | 281篇 |
2012年 | 470篇 |
2011年 | 519篇 |
2010年 | 306篇 |
2009年 | 246篇 |
2008年 | 426篇 |
2007年 | 501篇 |
2006年 | 491篇 |
2005年 | 490篇 |
2004年 | 481篇 |
2003年 | 501篇 |
2002年 | 474篇 |
2001年 | 83篇 |
2000年 | 56篇 |
1999年 | 90篇 |
1998年 | 124篇 |
1997年 | 81篇 |
1996年 | 69篇 |
1995年 | 73篇 |
1994年 | 65篇 |
1993年 | 53篇 |
1992年 | 42篇 |
1991年 | 40篇 |
1990年 | 36篇 |
1989年 | 43篇 |
1988年 | 20篇 |
1987年 | 22篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 13篇 |
1983年 | 7篇 |
1982年 | 18篇 |
1981年 | 9篇 |
1980年 | 12篇 |
1979年 | 13篇 |
1978年 | 8篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1973年 | 7篇 |
1969年 | 5篇 |
排序方式: 共有7566条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Kei Kamide Yoshihiro Kokubo Hironori Hanada Junko Nagura Jin Yang Shin Takiuchi Chihiro Tanaka Mariko Banno Yoshikazu Miwa Masayoshi Yoshii Tetsutaro Matayoshi Hisayo Yasuda Takeshi Horio Akira Okayama Hitonobu Tomoike Yuhei Kawano Toshiyuki Miyata 《Hypertension research》2006,29(4):243-252
Mutations in the gene encoding 11beta-hydroxysteroid dehydrogenase type 2, HSD11B2, cause a rare monogenic juvenile hypertensive syndrome called apparent mineralocorticoid excess (AME). In AME, defective HSD11B2 enzyme activity results in overstimulation of the mineralocorticoid receptor (MR) by cortisol, causing sodium retention, hypokalemia, and salt-dependent hypertension. Here, we have studied whether genetic variations in HDS11B2 are implicated in essential hypertension in Japanese hypertensives and the general population. By sequencing the entire coding region and the promoter region of HDS11B2 in 953 Japanese hypertensives, we identified five missense mutations in 11 patients (L14F, n = 5; R74H, n = 1; R147H, n = 3; T156I, n = 1; R335H, n = 1) and one novel frameshift mutation (4884Gdel, n = 1) in a heterozygous state, in addition to 19 genetic variations. All genetic variations identified were rare, with minor allele frequencies less than 0.005. Four of 12 patients with the missense/frameshift mutations showed renal failure. Four missense mutations, L14F, R74H, R147H, and R335H, were successfully genotyped in the general population, with a sample size of 3,655 individuals (2,175 normotensives and 1,480 hypertensives). Mutations L14F, R74H, R147H, and R335H were identified in hypertensives (n = 6, 8, 3, and 0, respectively) and normotensives (n = 8, 12, 5, and 0, respectively) with a similar frequency, suggesting that these missense mutations may not strongly affect the etiology of essential hypertension. Since the allele frequency of all of the genetic variations identified in this study was rare, an association study was not conducted. Taken together, our results indicate that missense mutations in HSD11B2 do not substantially contribute to essential hypertension in Japanese. 相似文献
5.
Hirotaka Koizuml Mikita Morita Shinya Mikaml Eiichi Shibayama Toshiyuki Uchikoshi 《Pathology international》1998,48(2):93-101
The Trk family of tyrosine protein kinase receptors plays a significant role in the development and maintenance of neural tissues. It has been recently shown that Trk receptors are also expressed by a wide range of normal non-neuronal tissues in humans in a cell type-specific manner. In the present study, the expression patterns of TrkA in 337 non-neuronal invasive carcinomas of 15 different human tissues were investigated immunohistochemically. Overall, 133 (39%), 101 (30%) and 103 (31%) tumors exhibited strong, moderate and no TrkA Immunoreactivity, respectively. Esophageal and thyroid carcinomas expressed high levels of TrkA, whereas the levels in gastric and colon cancers were low. TrkA expression was detected not only in carcinomas originating from TrkA-positive normal counterpart tissues, Including the esophagus, breast, lung and uterus, but also in those from TrkA-negative tissues/cells of the thyroid, liver and ovary. Immunostaining for nerve growth factor-β, the specific ligand for TrkA, in esophageal and breast carcinomas demonstrated its immunoreactivity in stromal fibroblasts and some TrkA-expressing tumor cells. These results suggest that paracrine/autocrine regulation via stromal/tumoral NGF-tumoral TrkA interaction may be involved In the growth of certain non-neuronal carcinomas. 相似文献
6.
Hisato Takagi Toshiyuki Tanabashi Norikazu Kawai Takuya Umemoto 《European journal of cardio-thoracic surgery》2007,32(2):400; author reply 400-400; author reply 401
7.
Objective To establish a means for prenatal prediction of spinal muscular atrophy (SMA) through survival motor neuron (SMN) gene deletion analysis and genetic counseling in families with a child affected with SMA.
Methods Genetic analysis for prenatal prediction of Werdnig-Hoffmann disease was performed in a at risk Chinese family by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) in SMN gene exons 7 and 8.
Results The pregnancy was positive for the homozygous deletion of the SMN gene, thus the fetus was diagnosed as being affected and the pregnancy was terminated.
Conclusion This approach is fast and reliable for DNA-based prenatal diagnosis of Werdnig-Hoffmann disease. 相似文献
Methods Genetic analysis for prenatal prediction of Werdnig-Hoffmann disease was performed in a at risk Chinese family by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) in SMN gene exons 7 and 8.
Results The pregnancy was positive for the homozygous deletion of the SMN gene, thus the fetus was diagnosed as being affected and the pregnancy was terminated.
Conclusion This approach is fast and reliable for DNA-based prenatal diagnosis of Werdnig-Hoffmann disease. 相似文献
8.
Imura Akihiro; Hori Toshiyuki; Imada Kazunori; Kawamata Shin; Tanaka Yuetsu; Imamura Sadao; Uchiyama Takashi 《Blood》1997,89(8):2951-2958
9.
Two-level posterior lumbar interbody fusion for degenerative disc disease: improved clinical outcome with restoration of lumbar lordosis 总被引:2,自引:0,他引:2
Akira Hioki MD Kei Miyamoto MD PhD Hirotaka Kodama MD PhD Hideo Hosoe MD PhD Hirofumi Nishimoto MD Hirofumi Sakaeda MD PhD Katsuji Shimizu MD DMSc 《The spine journal》2005,5(6):600-607
BACKGROUND CONTEXT: Although posterior lumbar interbody fusion (PLIF) for degenerative lumbar diseases is routine, there are few reports on double-level PLIF. PURPOSE: To evaluate the clinical outcomes of double-level PLIF. STUDY DESIGN/SETTING: A retrospective study of operated cases in Gifu, Japan. PATIENT SAMPLE: Nineteen patients (8 men and 11 women, 59.5+/-10.2 years) who underwent double-level PLIF between 1996 and 2001. OUTCOME MEASURES: Operation time, blood loss, complications, the Japanese Orthopaedic Association (JOA) score for back pain and lumbar sagittal alignment were evaluated. METHODS: Patients were examined retrospectively at follow-ups of 3.6+/-1.7 years. Primary diseases were spondylolisthesis, spinal canal stenosis, degenerative scoliosis and herniated intervertebral disc. Fusion areas were L3 to L5 in 15 cases and L4 to S1 in 4 cases. RESULTS: The mean JOA score increased from an initial score of 12.9+/-3.5 to 21.3+/-4.9 at the final follow-up. There was a positive correlation (R=0.718, p<.001) between the increase in lordotic angle and the increase in the JOA score. Several parameters suggested that the surgical invasiveness was not minimal. CONCLUSION: Double-level PLIF provided satisfactory results and preserved lumbar spine lordosis. 相似文献