全文获取类型
收费全文 | 7903篇 |
免费 | 375篇 |
国内免费 | 81篇 |
专业分类
耳鼻咽喉 | 28篇 |
儿科学 | 158篇 |
妇产科学 | 134篇 |
基础医学 | 1200篇 |
口腔科学 | 177篇 |
临床医学 | 495篇 |
内科学 | 1931篇 |
皮肤病学 | 261篇 |
神经病学 | 623篇 |
特种医学 | 284篇 |
外科学 | 1244篇 |
综合类 | 28篇 |
预防医学 | 228篇 |
眼科学 | 78篇 |
药学 | 559篇 |
中国医学 | 23篇 |
肿瘤学 | 908篇 |
出版年
2023年 | 32篇 |
2022年 | 96篇 |
2021年 | 163篇 |
2020年 | 87篇 |
2019年 | 114篇 |
2018年 | 163篇 |
2017年 | 162篇 |
2016年 | 181篇 |
2015年 | 179篇 |
2014年 | 259篇 |
2013年 | 299篇 |
2012年 | 514篇 |
2011年 | 562篇 |
2010年 | 323篇 |
2009年 | 257篇 |
2008年 | 454篇 |
2007年 | 536篇 |
2006年 | 537篇 |
2005年 | 523篇 |
2004年 | 504篇 |
2003年 | 529篇 |
2002年 | 510篇 |
2001年 | 106篇 |
2000年 | 81篇 |
1999年 | 111篇 |
1998年 | 136篇 |
1997年 | 89篇 |
1996年 | 73篇 |
1995年 | 78篇 |
1994年 | 71篇 |
1993年 | 65篇 |
1992年 | 55篇 |
1991年 | 60篇 |
1990年 | 55篇 |
1989年 | 64篇 |
1988年 | 37篇 |
1987年 | 36篇 |
1986年 | 16篇 |
1985年 | 25篇 |
1984年 | 21篇 |
1983年 | 11篇 |
1982年 | 22篇 |
1981年 | 9篇 |
1980年 | 12篇 |
1979年 | 18篇 |
1978年 | 13篇 |
1977年 | 8篇 |
1976年 | 10篇 |
1973年 | 13篇 |
1972年 | 7篇 |
排序方式: 共有8359条查询结果,搜索用时 0 毫秒
1.
2.
3.
Kei Kamide Yoshihiro Kokubo Hironori Hanada Junko Nagura Jin Yang Shin Takiuchi Chihiro Tanaka Mariko Banno Yoshikazu Miwa Masayoshi Yoshii Tetsutaro Matayoshi Hisayo Yasuda Takeshi Horio Akira Okayama Hitonobu Tomoike Yuhei Kawano Toshiyuki Miyata 《Hypertension research》2006,29(4):243-252
Mutations in the gene encoding 11beta-hydroxysteroid dehydrogenase type 2, HSD11B2, cause a rare monogenic juvenile hypertensive syndrome called apparent mineralocorticoid excess (AME). In AME, defective HSD11B2 enzyme activity results in overstimulation of the mineralocorticoid receptor (MR) by cortisol, causing sodium retention, hypokalemia, and salt-dependent hypertension. Here, we have studied whether genetic variations in HDS11B2 are implicated in essential hypertension in Japanese hypertensives and the general population. By sequencing the entire coding region and the promoter region of HDS11B2 in 953 Japanese hypertensives, we identified five missense mutations in 11 patients (L14F, n = 5; R74H, n = 1; R147H, n = 3; T156I, n = 1; R335H, n = 1) and one novel frameshift mutation (4884Gdel, n = 1) in a heterozygous state, in addition to 19 genetic variations. All genetic variations identified were rare, with minor allele frequencies less than 0.005. Four of 12 patients with the missense/frameshift mutations showed renal failure. Four missense mutations, L14F, R74H, R147H, and R335H, were successfully genotyped in the general population, with a sample size of 3,655 individuals (2,175 normotensives and 1,480 hypertensives). Mutations L14F, R74H, R147H, and R335H were identified in hypertensives (n = 6, 8, 3, and 0, respectively) and normotensives (n = 8, 12, 5, and 0, respectively) with a similar frequency, suggesting that these missense mutations may not strongly affect the etiology of essential hypertension. Since the allele frequency of all of the genetic variations identified in this study was rare, an association study was not conducted. Taken together, our results indicate that missense mutations in HSD11B2 do not substantially contribute to essential hypertension in Japanese. 相似文献
4.
Hisato Takagi Toshiyuki Tanabashi Norikazu Kawai Takuya Umemoto 《European journal of cardio-thoracic surgery》2007,32(2):400; author reply 400-400; author reply 401
5.
Objective To establish a means for prenatal prediction of spinal muscular atrophy (SMA) through survival motor neuron (SMN) gene deletion analysis and genetic counseling in families with a child affected with SMA.
Methods Genetic analysis for prenatal prediction of Werdnig-Hoffmann disease was performed in a at risk Chinese family by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) in SMN gene exons 7 and 8.
Results The pregnancy was positive for the homozygous deletion of the SMN gene, thus the fetus was diagnosed as being affected and the pregnancy was terminated.
Conclusion This approach is fast and reliable for DNA-based prenatal diagnosis of Werdnig-Hoffmann disease. 相似文献
Methods Genetic analysis for prenatal prediction of Werdnig-Hoffmann disease was performed in a at risk Chinese family by polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) in SMN gene exons 7 and 8.
Results The pregnancy was positive for the homozygous deletion of the SMN gene, thus the fetus was diagnosed as being affected and the pregnancy was terminated.
Conclusion This approach is fast and reliable for DNA-based prenatal diagnosis of Werdnig-Hoffmann disease. 相似文献
6.
Hiromitsu Nakaya Shuichi Kawashiri Akira Tanaka Natsuyo Noguchi Koroku Kato Takashi Hase Etsuhide Yamamoto 《Journal of oral pathology & medicine》2005,34(2):87-92
BACKGROUND: Although it is clear that dissemination via the blood system involves angiogenesis, it is uncertain whether tumors also induce lymphangiogenesis or simply invade existing peritumoral vessels. The purpose of this study was to elucidate changes in tumor blood and lymph vessels in cases involving the invasion of squamous cell carcinoma in the oral cavity, and its significance. Blood and lymph vessels densities in tongue carcinomas induced in hamsters were investigated. METHODS: Tongue cancer was induced by abrading the right margin of the tongue of each hamster with an endodontic barbed broach and subsequently applying 1.0% 9,10-dimenthl-1,2-benzanthracene (DMBA) dissolved in acetone, three times a week, at the same site. Fresh frozen sections were prepared and blood vessels stained blue by perfusion with Coomassie Brilliant Blue and lymph vessels stained brown for 5'-nucleotidase. The effects on the blood vessels and lymph vessels were observed. RESULTS: The results showed that blood and lymph vessel densities were greater in the advanced carcinoma tissues than in normal tissue. These were compared in terms of the mode of cancer invasion. As tumor invasion progressed, the blood vessel density decreased but lymph vessel density tended to be higher in high-degree tumor invasion than in low-degree tumor invasion. The expression of vascular endothelial growth factor-C was seen more frequently as tumor invasion progressed. CONCLUSIONS: The present findings indicated that angiogenesis and lymphangiogenesis are affected by cancerous invasion. 相似文献
7.
8.
9.
Y Hase T Kondo H Nakagawa T Matsumoto T Maekawa N Sakakibara 《Gan to kagaku ryoho. Cancer & chemotherapy》1987,14(11):3119-3124
As an intraoperative chemotherapy treatment for the regional lymph nodes in resectable cases of pancreatoduodenal cancer, Neocarzinostatin (NCS) was administered in the duodenal subserosa and the NCS concentration was measured in the resected lymph nodes. Experiments: NCS 4,000 units (n = 4), or 10,000 units (n = 4) was administered into the duodenal subserosa of mongrel dogs, and 2 hours after administration, the lymph nodes of the mesentery root were resected. The NCS concentration in the lymph nodes was 0.21 U/g in the 4,000-U group and 1.39 U/g in the 10,000-U group. Clinical findings: NCS 10,000 U was administered into the duodenal subserosa in 6 cases of pancreatoduodenal cancer. The total number of resected lymph nodes was 49 and the mean NCS concentration was 5.65 U/g. According to site, the highest concentration was measured in lymph nodes from the anterior and posterior region of the pancreas head, which were near to the administration site. Also, NCS was well distributed in the lymph nodes in the hepatoduodenal ligament and mesentery root which lay in the direction of lymph flow. NCS concentration was high in lymph nodes resected 1 hour after administration. According to experimental reports of in vitro studies, an NCS concentration of more than 0.5 U/g is required to obtain an anticancer effect. This method is therefore considered to be useful as a form of intraoperative chemotherapy for the regional lymph nodes in pancreatoduodenal cancer. 相似文献
10.
Q-Switched Alexandrite Laser Therapy for Pigmentation of the Lips Owing to Laugier-Hunziker Syndrome 总被引:2,自引:0,他引:2
Ozawa Toshiyuki MD Fujiwara Masao MD † Harada Teruichi MD Muraoka Michinari MD Ishii Masamitsu MD 《Dermatologic surgery》2005,31(6):709-712
BACKGROUND: Laugier-Hunziker (LH) syndrome is a rare benign condition in which hyperpigmentation of the lips and buccal mucosa occurs with no systemic associations. OBJECTIVE: We report the response to treatment with the Q-switched alexandrite laser (QSAL) because there are few reports on therapy for LH syndrome. METHODS: The QSAL was used for pigmentation of the lips in a 63-year-old woman with LH syndrome. Laser irradiation was done at 5.0 J/cm2 with a 3 mm spot size. RESULTS: There was 100% clearance of pigmentation of the lips with a single laser treatment, and recurrence was not observed after 6 months. CONCLUSION: The QSAL is very effective for pigmentation owing to LH syndrome. 相似文献