全文获取类型
收费全文 | 873篇 |
免费 | 35篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 8篇 |
妇产科学 | 1篇 |
基础医学 | 71篇 |
口腔科学 | 44篇 |
临床医学 | 41篇 |
内科学 | 150篇 |
皮肤病学 | 148篇 |
神经病学 | 94篇 |
特种医学 | 11篇 |
外科学 | 140篇 |
综合类 | 2篇 |
预防医学 | 16篇 |
眼科学 | 8篇 |
药学 | 56篇 |
中国医学 | 2篇 |
肿瘤学 | 117篇 |
出版年
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 18篇 |
2020年 | 9篇 |
2019年 | 13篇 |
2018年 | 13篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 13篇 |
2013年 | 17篇 |
2012年 | 35篇 |
2011年 | 36篇 |
2010年 | 16篇 |
2009年 | 24篇 |
2008年 | 53篇 |
2007年 | 50篇 |
2006年 | 38篇 |
2005年 | 33篇 |
2004年 | 33篇 |
2003年 | 38篇 |
2002年 | 38篇 |
2001年 | 46篇 |
2000年 | 36篇 |
1999年 | 38篇 |
1998年 | 22篇 |
1997年 | 10篇 |
1996年 | 13篇 |
1995年 | 8篇 |
1994年 | 14篇 |
1993年 | 3篇 |
1992年 | 19篇 |
1991年 | 14篇 |
1990年 | 21篇 |
1989年 | 15篇 |
1988年 | 26篇 |
1987年 | 23篇 |
1986年 | 15篇 |
1985年 | 17篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1981年 | 3篇 |
1979年 | 3篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1974年 | 4篇 |
1970年 | 3篇 |
1968年 | 2篇 |
1967年 | 4篇 |
1922年 | 2篇 |
排序方式: 共有909条查询结果,搜索用时 15 毫秒
1.
2.
Pathogenic role of connective tissue growth factor (CTGF/CCN2) in osteolytic metastasis of breast cancer. 总被引:5,自引:0,他引:5
3.
Shigeki Ohgitani Akimitsu Miyauchi Yasuyuki Takagi Yoshio Fujii Takuo Fujita Misa Nakamura Zhi-qiang Zhang Liang Shan Mitsuyo Sasaki Ryuichi Tsukino Toyoharu Yokoi Kennichi Kakudo Tomitaka Nakayama Junya Toguchida Hiroshi Kanoe Shinichi Aizawa Masao S. Sasaki Takashi Nakamura M. Suda K. Tanaka Y. Ogawa N. Tamura A. Yasoda H. Itoh M. Uehira N. Nishimoto T. Takigawa K. Shiota K. Nakao 《Journal of bone and mineral metabolism》1997,15(3):165-171
4.
5.
Regeneration of defects in articular cartilage in rat knee joints by CCN2 (connective tissue growth factor). 总被引:7,自引:0,他引:7
Takashi Nishida Satoshi Kubota Shunji Kojima Takuo Kuboki Kyouji Nakao Toshihiro Kushibiki Yasuhiko Tabata Masaharu Takigawa 《Journal of bone and mineral research》2004,19(8):1308-1319
CTGF/CCN2, a hypertrophic chondrocyte-specific gene product, possessed the ability to repair damaged articular cartilage in two animal models, which were experimental osteoarthritis and full-thickness defects of articular cartilage. These findings suggest that CTGF/CCN2 may be useful in regeneration of articular cartilage. INTRODUCTION: Connective tissue growth factor (CTGF)/CCN2 is a unique growth factor that stimulates the proliferation and differentiation, but not hypertrophy, of articular chondrocytes in vitro. The objective of this study was to investigate the therapeutic use of CTGF/CCN2. MATERIALS AND METHODS: The effects of recombinant CTGF/CCN2 (rCTGF/CCN2) on repair of damaged cartilage were evaluated by using both the monoiodoacetic acid (MIA)-induced experimental rat osteoarthritis (OA) model and full-thickness defects of rat articular cartilage in vivo. RESULTS: In the MIA-induced OA model, quantitative real-time RT-PCR assays showed a significant increase in the level of CTGF/CCN2 mRNA, and immunohistochemical analysis and in situ hybridization revealed that the clustered chondrocytes, in which clustering indicates an attempt to repair the damaged cartilage, produced CTGF/CCN2. Therefore, CTGF/CCN2 was suspected to play critical roles in cartilage repair. In fact, a single injection of rCTGF/CCN2 incorporated in gelatin hydrogel (rCTGF/CCN2-hydrogel) into the joint cavity of MIA-induced OA model rats repaired their articular cartilage to the extent that it became histologically similar to normal articular cartilage. Next, to examine the effect of rCTGF/CCN2 on the repair of articular cartilage, we created defects (2 mm in diameter) on the surface of articular cartilage in situ and implanted rCTGF/CCN2-hydrogel or PBS-hydrogel therein with collagen sponge. In the group implanted with rCTGF/CCN2-hydrogel collagen, new cartilage filled the defect 4 weeks postoperatively. In contrast, only soft tissue repair occurred when the PBS-hydrogel collagen was implanted. Consistent with these in vivo effects, rCTGF/CCN2 enhanced type II collagen and aggrecan mRNA expression in mouse bone marrow-derived stromal cells and induced chondrogenesis in vitro. CONCLUSION: These findings suggest the utility of CTGF/CCN2 in the regeneration of articular cartilage. 相似文献
6.
Hideo Kanauchi Sadao Imamura Masahiro Takigawa Fukumi Furukawa 《The Journal of dermatology》1994,21(12):935-939
Kampo, a Japanese-Chinese traditional herbal medicine, has been used for the treatment of various diseases for about 3,000 years in China. Among herbal medicines, Sairei-to is well known for improving the symptoms of rheumatoid arthritis (RA) and other collagen diseases. However, its immunosuppressive effects on autoimmune cutaneous phenomena are not completely understood. We investigated the effects of Sairei-to on the development of lupus dermatoses in autoimmune-prone MRL/Mp-lpr/lpr (MRL/lpr) mice, an animal model which spontaneously develops skin lesions similar to those seen in human lupus erythematosus. Virgin female MRL/lpr mice at 1 month of age, which were treated orally with Sairei-to, had reduced amounts of IgG deposition at the dermoepidermal junction, titers of anti-DNA antibodies and rheumatoid factor, and lymphoproliferation. These results support the use of traditional herbal medicines in patients with human RA and systemic lupus erythematosus. 相似文献
7.
J Fujita K Negayama K Takigawa Y Yamagishi A Kubo Y Yamaji J Takahara 《The Journal of antimicrobial chemotherapy》1992,29(5):539-546
The activity of 12 antibiotics, piperacillin, cefazolin, cefotiam, ceftizoxime, latamoxef, ceftazidime, cefuzonam, amikacin, ofloxacin, imipenem, aztreonam and minocycline, against 120 isolates of Pseudomonas aeruginosa was examined. In addition, the efficacy of antibiotics against single-, double-, or triple-drug-resistant isolates of P. aeruginosa were also examined to determine the cross-resistance to each drug. There was cross-resistance between piperacillin, ceftazidime and aztreonam, but amikacin and imipenem remained effective antibiotics, especially as salvage therapy, against isolates resistant to one agent. Results also suggested that piperacillin, ceftazidime or imipenem in combination with amikacin are effective combination regimens against most clinical isolates of P. aeruginosa. Amikacin and imipenem were also suitable antibiotics, especially as salvage therapy, against isolates of P. aeruginosa resistant to two agents. In conclusion, the results provide useful guidelines for choosing an effective treatment against clinical isolates of P. aeruginosa, and for choosing salvage therapy against resistant P. aeruginosa. 相似文献
8.
Kawada K Yonei T Ueoka H Kiura K Tabata M Takigawa N Harada M Tanimoto M 《Acta medica Okayama》2002,56(3):129-134
When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a substitute for the human tumor clonogenic assay (HTCA), we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939) and good in anthracyclines/anthracenedione (r = 0.611). However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan), and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione. 相似文献
9.
Lin Y Kawamura T Anno T Ichihara Y Ohta T Saito M Fujioka Y Kimura M Okada T Kuwayama Y Wakai K Ohno Y 《Environmental health and preventive medicine》1999,4(3):117-121
It is well known that physical exercise can reduce coronary risk factors. But how an aerobic exercise modifies coronary risk
factors in relation to severity and physical fitness is still controversial.
Fifty-four middle-aged women (mean age, 55 years) completed a 6-month on-site and home-based anaerobic threshold-level exercise
program. The changes in coronary risk factor profiles were observed during the pre-intervention and intervention periods.
Before the intervention (during control period), most coronary risk factors showed a rather unfavorable trend. After the program,
their mean body weight decreased from 56.7 to 55.7 kg (p>0.05) and the proportion of body fat from 30.9 to 27.9% (p>0.05)
without any reduction in lean body mass. Systolic blood pressure (SBP) decreased from 129.0 to 125.0 mm Hg (p>0.05) and diastolic
blood pressure from 79.5 to 76.6 mm Hg (p>0.05). Fasting plasma glucose (FPG) declined from 109.6 to 103.4 mg/dl (p>0.05).
Changes in SBP and FPG were most remarkable in their respective worst tertile. Serum lipids improved only modestly. Maximum
oxygen uptake increased from 23.6 to 26.1 ml/kg/min (p>0.01). However, no significant correlations were found between changes
in coronary risk factors and those in physical fitness. We conclude that the 6-month aerobic exercise program would modify
women’s coronary risk factors depending on their initial values, probably independently of the changes in physical fitness. 相似文献
10.
Akihiko Kimura Tomoji Uda Shoichi Nakashima Haruhiko Ikeda Seiji Yasuda Motoki Osawa Tsutomu Tsuji 《International journal of legal medicine》1993,106(1):1-4
Summary The erythrocyte band 3 (EPB3) variant, band 3 Memphis (EPB3*Memphis), was detected by immunoblotting with a monoclonal antibody to the 41 kDa cytoplasmic N-terminal domain of band 3 without protease treatment of erythrocytes. EPB3*Memphis was also detected by immunoblotting from 3-month-old bloodstains subjected to -chymotrypsin treatment. A population genetic study using this method indicated that the EPB3 variant would be useful for forensic work in Japan, since the frequency of this variant in Japanese (Wakayama prefecture) is relatively high (0.159). 相似文献