全文获取类型
收费全文 | 683篇 |
免费 | 45篇 |
国内免费 | 9篇 |
专业分类
儿科学 | 11篇 |
妇产科学 | 4篇 |
基础医学 | 48篇 |
口腔科学 | 2篇 |
临床医学 | 36篇 |
内科学 | 188篇 |
皮肤病学 | 13篇 |
神经病学 | 15篇 |
特种医学 | 23篇 |
外科学 | 227篇 |
预防医学 | 10篇 |
眼科学 | 7篇 |
药学 | 51篇 |
中国医学 | 5篇 |
肿瘤学 | 97篇 |
出版年
2024年 | 1篇 |
2023年 | 19篇 |
2022年 | 13篇 |
2021年 | 33篇 |
2020年 | 15篇 |
2019年 | 22篇 |
2018年 | 24篇 |
2017年 | 26篇 |
2016年 | 25篇 |
2015年 | 29篇 |
2014年 | 43篇 |
2013年 | 45篇 |
2012年 | 42篇 |
2011年 | 42篇 |
2010年 | 22篇 |
2009年 | 15篇 |
2008年 | 53篇 |
2007年 | 53篇 |
2006年 | 57篇 |
2005年 | 38篇 |
2004年 | 21篇 |
2003年 | 28篇 |
2002年 | 29篇 |
2001年 | 6篇 |
2000年 | 3篇 |
1999年 | 10篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
排序方式: 共有737条查询结果,搜索用时 15 毫秒
1.
Saiki O Uda H Nishimoto N Miwa T Mima T Ogawara T Azuma N Katada Y Sawaki J Tsutsui H Matsui K Maeda A Nakanishi K 《Clinical immunology (Orlando, Fla.)》2004,112(1):120-125
Adult Still's disease (ASD) is a chronic multisystemic disease. Extraordinarily high serum levels of IL-18 in ASD patients have been described, whereas the mechanism remains to be clarified. This study aimed to evaluate proinflammatory cytokines and to consider their pathological roles. In patients with rheumatic diseases (n = 151), blood samples were taken at the active phase and the serum levels of IL-18 and other proinflammatory cytokines were measured by ELISA. The extra-high levels of IL-18 were confirmed selectively in ASD patients (n = 10). In the active phase of ASD patients, the levels of IL-6 were elevated accordingly, but IL-1beta and TNF-alpha were undetectable. As to Th1-Th2 cytokines, the levels of IL-4 and IL-13, but not INF-gamma, IL-12, or IL-2, were elevated in all ASD patients examined. Moreover, the serum levels of IL-18 showed a good correlation with those of IL-4, suggesting that ASD reflects a Th2 rather than a Th1 cytokine profile. 相似文献
2.
Ichinose Y Okino T Yamasaki S Moriguchi Y Sugie T Li L Kanaoka S Kan N Watanabe Y Imamura M 《Surgery today》1999,29(4):338-343
To evaluate the effect of interferon-γ-genetransduced cells, DS mice were inoculated into their footpads with syngeneic mammary
adenocarcinoma SC42 admixed with interferon-γ producing mammary adenocarcinoma SC115Kγ, which had been established by an interferon-γ-gene
transduction in another syngeneic mammary adenocarcinoma SC115 using retroviral vectors. These mice rejected both tumor cells
and developed resistance to subsequent challenges with either SC115 or SC42 cells inoculated into their opposite posterior
footpads. These results thus indicate that systemic immunological memory to each of the independent tumor cell lines developed
in these mice. Although the SC42 cells admixed with irradiated SC115Kγ cells were rejected by these mice, the SC42 cells admixed
with irradiated SC115neoR, in which the neo-gene had been transduced, were observed to proliferate. Tumor rejection was reversed
by an in vivo administration of anti-interferon-γ antibody, thus suggesting that locally produced interferon-γ plays an important
role in tumor elimination and immunological memory induction. In conclusion, interferon-γ-gene-transduced tumor cells are
therefore considered to have a therapeutic potential for other types of malignant tumor cell lines. 相似文献
3.
Shigeki Wakiyama Katsuhiko Yanaga Yuji Soejima Takashi Nishizaki Tomoharu Yoshizumi Keizo Sugimachi 《Transplant international》1996,9(4):359-363
We studied the significance of N-acetyl--glucosaminidase (-NAG) and type III procollagen peptide (P-III-P) in the effluent of rodent hepatic grafts. After total hepatectomy, the livers were preserved in chilled, lactated Ringer's solution and then divided into five groups (n=10 each): group 1, 4 h preservation only; group 2, 4 h preservation and rewarming; group 3, 6 h preservation only; group 4, 6 h preservation and rewarming; and group 5, minimal preservation only. The -NAG of groups 2 and 4 was significantly higher than that of groups 1 and 3 (0.98±0.5 U/l vs 0.21±0.12 U/l; P<0.01 and 1.76±0.67 U/l vs 0.38±0.25 U/l, respectively; P<0.01), while that of group 4 was significantly higher than that of group 2 (1.76±0.67 U/l vs 0.98±0.50 U/l; P<0.05). The P-III-P of group 4 was significantly higher than that of group 2 (0.133±0.008 U/ml vs 0.110±0.015 U/ml; P<0.01). We conclude that -NAG is a novel parameter of parenchymal and nonparenchymal cells, while P-III-P reflects the integrity of the hepatic sinusoidal extracellular matrix. 相似文献
4.
Shigeyuki Kitamura Tomoharu Suzuki Tomoko Kadota Mayumi Yoshida Koji Ohashi Shigeru Ohta 《Drug metabolism and disposition》2003,31(2):179-186
The in vitro metabolism of fenthion and its sulfoxide (fenthion sulfoxide) in sea bream (Pagrus major) and goldfish (Carassius auratus) was investigated and compared with that in rats. Fenthion was oxidized to fenthion sulfoxide and the oxon derivative, but not to its sulfone, in the presence of NADPH by liver microsomes of sea bream, goldfish, and rats. These liver microsomal activities of the fish were lower than those of rats but were of the same order of magnitude. The NADPH-linked oxon- and sulfoxide-forming activities of liver microsomes of the fish and rats were inhibited by SKF 525-A, metyrapone, alpha-naphthoflavone, and carbon monoxide. The oxidizing activity to fenthion sulfoxide was also inhibited by alpha-naphthylthiourea. Several cytochrome P450 isoforms and flavin-containing monooxygenase 1 exhibited these oxidase activities. Fenthion sulfoxide was reduced to fenthion with liver cytosol of the fish and rats upon addition of 2-hydroxypyrimidine, N(1)-methylnicotinamide, or butyraldehyde, each of which is an electron donor of aldehyde oxidase, under anaerobic conditions. The activity was inhibited by menadione, beta-estradiol, and chlorpromazine, which are inhibitors of aldehyde oxidase. The activities in the fish livers were similar to those of rat liver. Aldehyde oxidase purified from the livers of sea bream and rats exhibited the reducing activity. Thus, fenthion and fenthion sulfoxide are interconvertible in fish and rats through the activities of cytochrome P450, flavin-containing monooxygenase, and aldehyde oxidase. 相似文献
5.
A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50% methanol containing 1% formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification. 相似文献
6.
Naoko Iwahashi Kondo Ken Shirabe Yohei Mano Akinobu Taketomi Tomoharu Yoshizumi Toru Ikegami Toshiro Masuda Hiroto Kayashima Naotaka Hashimoto Kazutoyo Morita Mizue Matsuo Yoshihiko Maehara 《Surgery today》2012,42(12):1210-1214
The outcome after surgical resection for intrahepatic cholangiocarcinoma has not been satisfactorily evaluated due to its malignant behavior. Surgical resection, however, has the potential to improve the prognosis and may allow surgeons to experience rare cases with long survival. This report presents the case of a patient who developed recurrence 9?years after resection of intrahepatic cholangiocarcinoma. A 76-year-old female was diagnosed to have intrahepatic cholangiocarcinoma and underwent an extended right posterior subsegmentectomy. The gross appearance showed a mass-forming type tumor. The histopathological examination revealed well to moderately differentiated adenocarcinoma associated with portal vein invasion. Subcutaneous metastasis in the head as the first sign of relapse was diagnosed 9?years after hepatectomy. The histopathological findings of the subcutaneous tumor were similar to those of the intrahepatic cholangiocarcinoma, thus suggesting metastasis from intrahepatic cholangiocarcinoma. Positron emission tomography with 2-[fluorine-18]-fluoro-2-deoxy-d-glucose was useful for detecting multiple metastases. Long-term follow-up for more than 5?years is recommended because the present case shows that late recurrence of intrahepatic cholangiocarcinoma occurs even 5?years after resection. 相似文献
7.
Phosphodiesterase III inhibitor attenuates rat sinusoidal obstruction syndrome through inhibition of platelet aggregation in Disse's space 下载免费PDF全文
8.
Yoshinobu Nagasawa Tomoharu Shimizu Hiromichi Sonoda Hirotomi Chou Eiji Mekata Tohru Tani 《Surgery today》2014,44(7):1346-1349
Catheter rupture after totally implantable access port (TIAP) implantation via the right internal jugular vein is thought to be very rare. We report a case of catheter rupture found 682 days after TIAP surgery in a 52-year-old woman with recurrent right breast cancer. It is possible that chronic stress at the flexure of the catheter induced by neck movements caused the catheter to rupture. Therefore, when inserting a TIAP via the right internal jugular vein, the site of venous puncture should be decided on carefully. Although a fracture of this type is rarely reported in the literature, the incidence of catheter injury of a TIAP inserted via the internal jugular vein at our institute is 1.8 %. This highlights the need to educate and caution medical staff and patients about preventing catheter fracture being caused by external factors. 相似文献
9.