全文获取类型
收费全文 | 189篇 |
免费 | 22篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 4篇 |
妇产科学 | 4篇 |
基础医学 | 27篇 |
口腔科学 | 5篇 |
临床医学 | 24篇 |
内科学 | 46篇 |
皮肤病学 | 2篇 |
神经病学 | 11篇 |
外科学 | 21篇 |
综合类 | 1篇 |
预防医学 | 26篇 |
眼科学 | 11篇 |
药学 | 13篇 |
中国医学 | 5篇 |
肿瘤学 | 9篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 13篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 6篇 |
2017年 | 14篇 |
2016年 | 17篇 |
2015年 | 4篇 |
2014年 | 9篇 |
2013年 | 11篇 |
2012年 | 11篇 |
2011年 | 11篇 |
2010年 | 9篇 |
2009年 | 6篇 |
2008年 | 10篇 |
2007年 | 9篇 |
2006年 | 13篇 |
2005年 | 13篇 |
2004年 | 6篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1993年 | 1篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1981年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1968年 | 2篇 |
排序方式: 共有211条查询结果,搜索用时 15 毫秒
1.
Cortés-Gutiérrez EI Cerda-Flores RM González-Ramírez D Zúñiga-Charles MA Lazcano-Martínez S Sampayo-Reyes A Leal-Garza CH 《Mutagenesis》2004,19(3):203-205
The aims of this study were to evaluate the mutagenic and cytotoxic activity of mercurous chloride by the micronucleus technique in vivo on the bone marrow of golden Syrian hamsters after a single i.p. drug administration. Forty male golden Syrian hamsters were classified into eight groups: negative control, positive control and six groups treated with different doses of mercurous chloride (1.25, 2.5, 5, 10, 20 and 40 mg/kg). The negative control was injected with physiological saline i.p. and the positive control with cyclophosphamide at a dose of 80 mg/kg i.p. With respect to mutagenic effect, the average number of micronucleated polychromatic erythrocytes (MPE) in hamsters treated with different doses of mercurous chloride was not significant compared with the negative control. With respect to cytotoxic effect, the average polychromatic erythrocyte/red blood cell ratio showed a significant decrease when the doses were higher than the 2.5 mg/kg dose compared with the negative control. In conclusion, this preliminary study shows a cytotoxic effect but not a mutagenic effect of calomel in vivo at one time point (24 h). 相似文献
2.
María Elena Escobar Ospina Jonatan Gómez Perdomo 《Artificial intelligence in medicine》2013,57(1):31-47
ObjectiveThis paper presents a conceptual model that is developed upon a characterization of human papillomavirus type 16 (HPV16) which is used to build a simulation prototype of the HPV16 growth process.MethodologyThe human papillomavirus type 16 is the principal virus detected in invasive lesions of cervical cancer, and associated with the greater persistence and prevalence in pre-malignant and malignant lesions. The probability of acquiring an infection with HPV16 is extremely high in sexually active individuals. However, an HPV16 infection can disappear after becoming a histological confirmed case. According to the characterization of HPV16 proposed in this paper, cells as compared to a society behaves as a complex system, i.e., cells behave in a cooperative manner, following a set of rules defined by local interactions among them. Such complex system is defined by combining a cellular automaton and agent-based models. In this way, the behavior of the HPV16 is simulated by allowing the cellular automaton to follow such parameterized behavior rules.ResultsBoth cross-sectional and prospective studies indicate that HPV16 infection persistence increase the risk of high-grade CIN, as observed in the results provided by the growth simulation model of HPV16. The average growth rate extrapolated over 52 weeks (12 months) and calculated by the model showed a 37.87% growth for CIN1, 35.53% for CIN2 and 16.92% for CIN3. Remarkably, these results are similar to the results obtained and reported by clinical studies. For example, the results obtained using the proposed model for CIN2 and the results obtained by Östör [36], have a differential of 0.53 percentage points while have a differential of 2.23 percentage points with the results obtained by Insinga et al. [51]. Also, for the CIN3, the results obtained using the proposed model, have a differential of 2.92 percentage points with the Insinga et al. [52], results.ConclusionThrough the specification of parameterized behavior rules for HPV16 that are simulated under the combined technique of cellular automata and agent-based models, the HPV life cycle can be simulated allowing for observations at different stages. The proposed model then can be used as a support tool in the investigation of HPV16, in particular (as part of our future work) to develop drugs as agents in the control of the HPV16 disease. 相似文献
3.
Sigifredo Pedraza-Sánchez Amy G. Hise Lakshmi Ramachandra Fabian Arechavaleta-Velasco Christopher L. King 《Clinical and Vaccine Immunology : CVI》2013,20(7):962-971
The human innate immune response to pathogens is not fully effective and mature until well into childhood, as exemplified by various responses to Toll-like receptor (TLR) agonists in newborns compared to adults. To better understand the mechanistic basis for this age-related difference in innate immunity, we compared tumor necrosis factor alpha (TNF-α) production by monocytes from cord blood (CB) and adult blood (AB) in response to LAM (lipoarabinomannan from Mycobacterium tuberculosis, a TLR2 ligand) and LPS (lipopolysaccharide from Escherichia coli, a TLR4 ligand). LPS or LAM-induced TNF-α production was 5 to 18 times higher in AB than in CB monocytes, whereas interleukin-1α (IL-1α) stimulated similar levels of TNF-α in both groups, suggesting that decreased responses to LPS or LAM in CB are unlikely to be due to differences in the MyD88-dependent signaling pathway. This impaired signaling was attributable, in part, to lower functional TLR4 expression, especially on CD14+ CD16+ monocytes, which are the primary cell subset for LPS-induced TNF-α production. Importantly, the frequency of CD14+ CD16+ monocytes in CB was 2.5-fold lower than in AB (P < 0.01). CB from Kenyan newborns sensitized to parasite antigens in utero had more CD14+ CD16+ monocytes (P = 0.02) and produced higher levels of TNF-α in response to LPS (P = 0.004) than CB from unsensitized Kenyan or North American newborns. Thus, a reduced CD14+ CD16+ activated/differentiated monocyte subset and a correspondingly lower level of functional TLR4 on monocytes contributes to the relatively low TNF-α response to LPS observed in immunologically naive newborns compared to the response in adults. 相似文献
4.
Botero JE Yepes FL Roldán N Castrillón CA Hincapie JP Ochoa SP Ospina CA Becerra MA Jaramillo A Gutierrez SJ Contreras A 《Journal of periodontology》2012,83(10):1245-1250
Background: Periodontal disease has been associated with diabetes, but there is still controversy on the relationship between periodontal clinical parameters and glycemic control. The purpose of this study is to assess the relationship between blood glucose levels and clinical parameters of periodontal disease in individuals with diabetes. Methods: A total of 65 individuals with diabetes and 81 individuals without diabetes were included in the study. A full-mouth periodontal examination and preprandial fasting glycemia values were recorded for each individual. Glycosylated hemoglobin was only measured in patients with diabetes. A comparative analysis between groups (Mann-Whitney U test) and a correlation analysis between glycemia and periodontal parameters were performed (Spearman test). Results: Patients without diabetes presented more teeth than individuals with diabetes (P <0.05). Patients with diabetes with periodontitis displayed loss of periodontal clinical attachment compared to patients without diabetes, but the highest value was observed in patients with periodontitis that reported a smoking habit. Furthermore, patients with diabetes with periodontitis presented higher glycemia and glycated hemoglobin values in contrast to patients with gingivitis. Patients with diabetes with hyperglycemia had a higher risk to develop periodontitis (odds ratio = 2.24; 95% confidence interval = 1.02 to 4.93). A positive correlation was observed between glycemia and clinical attachment loss (AL), whereas a negative correlation between glycemia and the number of teeth present was found (P <0.05). Conclusions: Tooth and periodontal AL were increased by hyperglycemia in individuals with diabetes. This study contributes additional evidence that diabetes could aggravate periodontal disease and affect the systemic health of individuals. 相似文献
5.
Gregory J. Walker Zin Naing Alberto Ospina Stella Malinna Yeang Joanna Caguicla Vidiya Ramachandran Sonia R. Isaacs David Agapiou Rowena A. Bull Sacha Stelzer-Braid James Daly Iain B. Gosbell Veronica C. Hoad David O. Irving Joanne M. Pink Stuart Turville Anthony D. Kelleher William D. Rawlinson 《Viruses》2021,13(2)
Serological testing for SARS-CoV-2-specific antibodies provides important research and diagnostic information relating to COVID-19 prevalence, incidence and host immune response. A greater understanding of the relationship between functionally neutralising antibodies detected using microneutralisation assays and binding antibodies detected using scalable enzyme immunoassays (EIA) is needed in order to address protective immunity post-infection or vaccination, and assess EIA suitability as a surrogate test for screening of convalescent plasma donors. We assessed whether neutralising antibody titres correlated with signal cut-off ratios in five commercially available EIAs, and one in-house assay based on expressed spike protein targets. Sera from recovered patients or convalescent plasma donors who reported laboratory-confirmed SARS-CoV-2 infection (n = 200), and negative control sera collected prior to the COVID-19 pandemic (n = 100), were assessed in parallel. Performance was assessed by calculating EIA sensitivity and specificity with reference to microneutralisation. Neutralising antibodies were detected in 166 (83%) samples. Compared with this, the most sensitive EIAs were the Cobas Elecsys Anti-SARS-CoV-2 (98%) and Vitros Immunodiagnostic Anti-SARS-CoV-2 (100%), which detect total antibody targeting the N and S1 antigens, respectively. The assay with the best quantitative relationship with microneutralisation was the Euroimmun IgG. These results suggest the marker used (total Ab vs. IgG vs. IgA) and the target antigen are important determinants of assay performance. The strong correlation between microneutralisation and some commercially available assays demonstrates their potential for clinical and research use in assessing protection following infection or vaccination, and use as a surrogate test to assess donor suitability for convalescent plasma donation. 相似文献
6.
在一项随机、平行分组的开放试验中,27例妊娠糖尿病妇女(年龄30.7±6.3岁,HbA1c〈7%)随机分为门冬胰岛素治疗组(餐前5分钟注射)和常规人胰岛素治疗组(餐前30分钟注射)。试验时间为从诊断妊娠糖尿病(18~28周)至产后6周。研究期间两组的总体血糖水平均控制良好(试验开始和结束时HbA1c≤6%)。进餐试验时,试验6周时的平均血糖水平(门冬胰岛素组4.2±0.57mmol/L,常规人胰岛素组4.8±0.86mmol/L)略低于试验0周时(门冬胰岛素组4.9±0.59mmol/L,常规人胰岛素组5.1±0.36mmol/L)。 相似文献
7.
8.
Comparison of an insulin analog,insulin aspart,and regular human insulin with no insulin in gestational diabetes mellitus 总被引:5,自引:0,他引:5
OBJECTIVE: To assess the short-term efficacy of insulin aspart in comparison with regular human insulin in women with gestational diabetes mellitus (GDM) during standardized meal tests. RESEARCH DESIGN AND METHODS: The study included 15 women with GDM who had inadequate diabetes control with diet alone. On 3 consecutive days, breakfast meal tests were performed-the first with no exogenous insulin and the other two after the injection of either regular insulin or insulin aspart. RESULTS: The peak insulin concentration was higher and the peak glucose and C-peptide concentrations were lower with both insulin preparations than with no exogenous insulin. Glucose areas under the curve above baseline were significantly lower with insulin aspart (180-min area, 7.1 mg. h. dl(-1); P = 0.018), but not with regular insulin (30.2 mg. h. dl(-1); P = 0.997), than with no insulin (29.4 mg. h. dl(-1)). CONCLUSIONS: This study demonstrates that effective postprandial glycemic control in women with GDM who required insulin was brought about by insulin aspart through higher insulin peak and lower demand on endogenous insulin secretion. 相似文献
9.
Bernardo Rocco Barbara A. Jereczek‐Fossa Deliu‐Victor Matei Fabrizio Verweij Luigi Santoro Andrea Vavassori Juan Camillo Ospina Francisco Cedeira Mario Ciocca Roberto Orecchia Ottavio De Cobelli 《BJU international》2009,104(11):1624-1630
OBJECTIVE
To evaluate a novel approach with intraoperative radiotherapy (IORT) administered in the surgical field, after pelvic lymphadenectomy (PL) and before radical retropubic prostatectomy (RRP), evaluating acute and late toxicity, complications and biochemical progression‐free survival (bPFS), as the adequate treatment of locally advanced prostate cancer is still a controversial issue.PATIENTS AND METHODS
Between June 2005 and October 2007, 33 consecutive patients with intermediate‐risk or locally advanced prostate cancer were selected for PL + IORT + RRP. IORT was delivered by a mobile linear accelerator in the operating room (electron beam, 12 Gy at 90% isodose). According to the pathological findings further adjuvant radio‐ or hormone therapy could be administered. The median follow‐up was 16 months. This group was compared retrospectively with a historical group of 100 patients who had undergone RRP and further adjuvant therapy, selected with equivalent criteria. The comparison was conducted as a matched‐pair analysis. The perioperative outcomes (surgical time, estimated blood loss, blood transfusions, days of catheterization, days of drainage, days of hospitalization), continence as the functional outcome, acute and late toxicity, rate of complications and bPFS were evaluated and compared.RESULTS
The baseline characteristics of the two groups were equivalent but the node count and the number of positive lymph nodes was higher in the IORT group. The IORT group had longer surgery, and a shorter hospital stay and catheterization. There were no differences in continence rate, and no major complications in either group. The acute and late toxicity and bPFS were equivalent. A retrospective comparison and the short follow‐up were the major limitations.CONCLUSIONS
IORT administered before RRP seems a feasible approach, with little effect on the variables evaluated. 相似文献10.
Marta C. Ospina Francisco J. Diaz Jorge E. Osorio 《The American journal of tropical medicine and hygiene》2010,83(3):672-678
During the past two decades, Dengue virus-3 (DENV-3) has re-emerged in the Western Hemisphere causing significant epidemics of dengue fever (DF) and dengue hemorrhagic fever (DHF). In an effort to understand the molecular evolution of DENV-3 and their relationships to other DENV-3 circulating in the western hemisphere, we conducted a phylogenetic study on DENV-3 isolates made between 2002 and 2007 in the metropolitan area of Medellín, Colombia. An unexpected co-circulation of two different variants of DENV-3 subtype III during at least 5 years in Medellín was found. In addition, a more complete analysis of DENV-3 viruses isolated in other South American regions revealed the existence of three different subtype III lineages, all derived from independent introductions. This study documents significant genetic diversity of circulating viruses within the same subtype and an unusual capacity of the population of this city to support continuous circulation of multiple variants of dengue virus. 相似文献