Assessment and upgrading of Alexandria University nursing students' knowledge and attitudes about genetic counseling

The aim of this study was to study the effect of a teaching unit on upgrading university-nursing students' knowledge and attitudes about genetic counseling. The study used pre- test, posttest with no control group design. All nursing students (100) who accepted to participate in the study and enrolled in the Maternity and Gynecological-nursing course, during the second semester of the third academic year 1999-2000, at the Faculty of Nursing, Alexandria University, were involved in the study. A self-administered questionnaire sheet was used to assess nursing students' knowledge about genetic counseling, while a 3-point Likert-like scale was used to assess their attitudes towards it. The content of six-hours' teaching unit -about genetic counseling was taught to students by the researchers based on their needs. The study revealed a general lack of knowledge among nursing students regarding basic information about genetic counseling, where poor total score of knowledge was obtained by all of them in the pre-test. In addition, the pre-test revealed that negative attitude was found in about half of the nursing students. The teaching unit had an obvious effect on the nursing students' knowledge and attitudes regarding genetic counseling, as the total score of their knowledge and attitudes had improved after implementation of the teaching unit. Moreover, there was a significant relationship between nursing students' knowledge and attitudes towards genetic counseling before and after implementation of the teaching unit. Therefore, a negative attitude was found with poor total score of knowledge in the pre-test and positive attitude was found with good total score of knowledge in the post-test. So, there is a need to improve knowledge and change attitude of nursing students about genetic counseling. As well as genetic counseling should be included in the university-nursing curriculum and should be reviewed periodically to accommodate the relevant fast change in the science and technology of genetics. Moreover, nursing students must be informed about the different community resources, which offer the genetic counseling services.     

Molecular characterization of CTX-Mβ-lactamases among Klebsiella pneumoniae isolated from patients at Tehran hospitals

Purpose: Plasmid-encoded CTX-M-group of extended-spectrum β-lactamases (ESBLs) represent a significant and rapidly emerging problem in most part of the world. The aim of the present study was to describe the prevalence of CTX-M producing Klebsiella pneumoniae at Tehran hospitals. Materials and Methods: Clinical isolates of K. pneumoniae (n=250) were collected from 10 hospitals of Tehran. Susceptibility to antimicrobial agents, MIC of cefotaxime and ESBLs production of collected isolates were detected. All ESBL-producing isolates were screened for bla_CTX-M genes using PCR and DNA sequencing. Molecular typing of bla_CTX-M harboring isolates was performed by Pulsed–field gel electrophoresis assay. Results: Of 250 K. pneumoniae clinical isolates, 102 isolates revealed ESBLs – phenotype. PCR assay and sequencing detected bla_CTX-M genes in 71.5% (n= 73) of ESBL-producing isolates. The prevalence of CTX-M -I and CTX-M-III clusters among these isolates was 35.61% (n=26) and 21.9 % (n=16) respectively. Coexistence of CTX-M -I and CTX-M-III clusters was found among 42.5% (n= 31) of isolates. Of 102 isolates that were positive in the phenotypic confirmatory test (PCT), 29 isolates (28.4%) did not produce any amplicons in PCR for bla_CTX-M gene. The results of PCR for CTX-M -II and CTX-M-IV clusters were also negative. Analysis of the 31 CTX-M producing K. pneumoniae isolates by PFGE typing showed 26 distinct patterns. Conclusions: The bla_CTX-M genes are widespread among Iranian isolates of K. pneumoniae. PFGE demonstrated the high diversity of K. pneumoniae harboring bla_CTX-M in our study.     

Immunolocalization of Glioma-Associated Oncogene Homolog 1 in Non Melanoma Skin Cancer

Glioma-associated oncogene homolog (GLI)1 is involved in controlling cell proliferation and angiogenesis. The aim of this work was to explore its possible role in non-melanoma skin cancer pathogenesis through its immunohistochemical (IHC) expression in skin biopsies of these diseases and correlating this expression with the clinico-pathological parameters of the studied cases. Seventy-six cutaneous specimens were studied; 30 cases with basal cell carcinoma (BCC), 30 cases with squamous cell carcinoma (SCC) and 16 normal skin samples, from age- and gender-matched subjects, as a control group. GLI1 was expressed in all BCC cases and in 60% of SCC cases. All SCC cases showed cytoplasmic, while 70% of BCC cases showed nucleocytoplasmic immunoreactivity. It was over expressed in BCC and SCC compared to normal skin (p?=?0.01 and 0.0006, respectively). Higher Histo (H) score in BCC cases was significantly associated with female gender (p?=?0.04), multiple lesions, desmoplastic stromal reaction and stromal angiogenesis (p?p?=?0.004 for all), inflammatory stromal reaction (p?=?0.01), lymph node involvement and absence of calcification (p?=?0.001 for both). In conclusion, GLI1 may play a role in BCC pathogenesis through its role in cell proliferation, migration, and angiogenesis. Its upregulation and cytoplasmic localization in SCC may suggest that its role in tumor pathogenesis is through mechanisms other than Hedgehog pathway activation. Further studies are needed to clarify the exact molecular basis of its oncogenic action.     

Nuclear Factor Kappa B and Cyclo-Oxygenase-2: Two Concordant Players in Psoriasis Pathogenesis

Nuclear factor kappa B (NFκB) is a key regulatory element in a variety of immune and inflammatory pathways, cellular proliferation, differentiation and apoptosis. Cyclo-oxygenase 2 (COX2) is one of the downstream targets of NFκB. The current work aimed to explore the possible role of NFκB and COX2 in psoriasis pathogenesis through their immunohistochemical (IHC) expression in skin biopsies of this disease and correlating this expression with clinico-pathological parameters of studied cases. 103 subjects were studied; including 58 cases with psoriasis vulgaris (lesional and perilesional skin) and 45 normal, age- and gender-matched subjects, as a control group. NFκB and COX2 expressions were evaluated using standard IHC techniques. NFκB and COX2 were upregulated in psoriasis lesional skin compared to perilesional (p?p?p?=?0.02 for both), severe degree of perivascular inflammatory infiltrate (p?=?0.03 and 0.002, respectively) and thin suprapapillary epidermis (p?=?0.003 and 0.006, respectively). Significant positive correlation was noted between NFκB and COX2 H scores in epidermis (r?=?0.41, p?=?0.02) and dermis (r?=?0.6, p?=?0.04) of lesional skin. Significant positive correlation between NFκB H score and PASI score (r?=?0.38, p?=?0.04) and between COX2 H score and PASI score (r?=?0.52, p?相似文献   

Analysis of methodology for measurement of intramyocardial pressure

Summary We critically evaluated the major techniques for measurement of intramyocardial pressure (IMP (closed, perfusion, open, microtransducer). Each technique demonstrates a gradient in systolic IMP increasing with depth from the epicardium. The estimated magnitude of this gradient varies with the technique employed. Indirect methods (closed, perfusion) estimate a higher value for the systolic IMP gradient. The open and microtransducer methods, which measure a direct hydrostatic pressure within the myocardium, are less invasive and reflect more reliably the magnitude of IMP. The results for the open and microtransducer methods were comparable and indicate that normalized systolic IMP increases linearly from the epicardium. In the inner half of the myocardium systolic IMP approaches the level of systolic LVP and in the subendocardial layer it exceeds the systolic LVP by about 8%. A reverse IMP gradient occurred in late diastole ranging from 5 mm Hg in the subendocardial region K 25 mm Hg in the subepicardium.     

Double anterior chamber in a patient with glaucoma and microspherophakia

We report the case of a 16-year-old woman with microspherophakia and secondary open angle glaucoma. The patient presented with a membrane dividing the anterior chamber into two segments without edema or Descemet's membrane detachment. Slit lamp biomicroscopy, Pentacam, and specular microscopy images were obtained. Double anterior chamber is primarily found in patients with anterior chamber anomalies when there is no history of surgery or trauma.