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1.
Fahad Ali Rahul Smita Jyoti Ambreen Fatima Saba Khanam Falaq Naz Yasir Hasan Siddique 《Scientia pharmaceutica》2016,84(2):361-377
The present investigation was aimed at studying the possible role of curcumin against N-nitrosodiethylamine (NDEA)-induced toxicity in albino rats. Administration of NDEA to rats at a concentration of 0.1 mg/ml in drinking water ad libitum for 21 days produced toxicity in them, which was evident from histopathological changes in the rat livers, and increased levels of blood serum enzyme markers, i.e. aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate dehydrogenase. In addition, the levels of oxidative stress markers like lipid peroxidation (LPO), protein carbonyl (PCC), and glutathione-S-transferase (GST) activity were elevated and the total glutathione (GSH) content was reduced in the livers. The administration of curcumin to rats at concentrations of 10, 20, and 40 mg/ml in drinking water along with 0.1 mg/ml of NDEA for 21 days effectively suppressed NDEA-induced toxicity and also resulted in a dose-dependent reduction in the levels of blood serum enzyme markers (AST, ALT, ALP, and LDH). Moreover, LPO, PCC, and GST activity were reduced and the GSH level was increased upon the administration of curcumin along with NDEA. The results obtained for the comet assay in rat hepatocytes and blood lymphocytes showed a significant dose-dependent decrease in the mean tail length. The micronucleus assay performed on rat hepatocytes also showed a dose-dependent reduction in the frequency of micronucleated cells along with curcumin administration. These results suggest that curcumin has a protective role against NDEA-induced toxicity in albino rats. 相似文献
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J S Appelbaum R P Roos E F Salazar-Grueso A Buchman S Iannaccone R Glantz T Siddique R Maselli 《Neurology》1992,42(8):1488-1492
Although there are varied inheritance patterns in motor neuron disease (MND), the phenotype of MND is reported to be constant within these families, ie, cases of amyotrophic lateral sclerosis or primary lateral sclerosis do not occur in pedigrees with cases of spinal muscular atrophy. We describe four pedigrees whose members diverged in the phenotype of MND expressed. The intrafamilial variation of phenotype suggests a similar pathogenesis for some of the varied types of familial MND and the need for careful inquiry of family history in all patients with MND. 相似文献
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Campylobacter jejuni has been documented as one of the major aetiological agents of diarrhoeal illness all over the world. Studies revealed its pathogenicity by different assay methods, but none could be strongly recommended as a tool for differentiating toxigenic strains of C. jejuni. This study was an attempt to demonstrate better its pathogenicity by media modification. Fifteen isolates of C. jejuni recovered from diarrhoeal patients at the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka Hospital were included in this study. The standard medium for C. jejuni was modified by the incorporation of FeCl3 at different concentrations. The pathogenicity of the test isolates were studied by rabbit ileal loop assay; Chinese hamster ovary (CHO), human epithelial cervical carcinoma (HeLa); Y-1 adrenal cell lines and suckling mouse assay. Sonicated extracts of the test organisms, grown with FeCl3 supplement, were also assayed. An enhanced growth of C. jejuni was obtained with the increasing concentration of FeCl3 supplementation in the medium. Only five isolates of C. jejuni produced cytotoxic effect on HeLa cell monolayer. Other cell lines were not affected by the test specimens or sonicates. Rabbit ileal loop assay did not reveal any fluid accumulation but on dissection, the test loops were found highly haemorrhagic. No heat-stable (ST) toxin could be detected. Cell-free culture supernatant of patients' isolates of C. jejuni had an effect on HeLa cell monolayer. Sonicated extracts of patients' extracts had a greater effect on HeLa cell monolayer. Pathogenic strains of C. jejuni might be distinguished on HeLa cell monolayer using its sonicated extracts. 相似文献
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We describe a 28-year-old white Caucasian man displaying many of the physical signs of ectodermal dysplasia (ED). An unusual finding was his presentation with xerostomia. Salivary gland imaging techniques revealed aplasia of both submandibular salivary glands and relatively small parotids. The case highlights that hypoplasia and aplasia of exocrine glands could be rare features of ED. In the management of ED, early detection of xerostomia is important to limit any potential damage to the already hypodontic dentition. 相似文献
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The aim of this study was to describe the effects of aluminum on the stromal cells of rat bone marrow using a combination of in vivo exposure and in vitro culture of bone marrow. The toxic effects of aluminum are manifested by a decrease in the erythrocyte count and hemoglobin level in the peripheral blood. Aluminum stimulated an increase in the number of fibroblasts while the macrophage count dropped. The number of adipocytes remained unaffected. An increase in mean corpuscular hemoglobin (MCH) was also found in the peripheral blood of experimental animals. 相似文献
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A glycoprotein (gp-88) secreted by pseudorabies virus (PRV) infected cells was isolated and purified. Anti gp-88 antibodies were used to screen an expression library constructed in Escherichia coli using genomic PRV DNA. Two positive clones were identified and the cloned genetic information was used to localize the corresponding gene in the unique short region of the PRV genome. Antibodies to the glycoprotein were also used to identify the unglycosylated precursor as synthesized in an in vitro translation system. A major precursor of 65 kDa was detected. Although the glycoprotein described here was not found as a major structural glycoprotein of the virion, antibodies to gp-88 are able to neutralise viral activity in vitro. The possible relationship with known glycoproteins of PRV is discussed. 相似文献
10.
New variants of Vibrio cholerae O1 biotype El Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh
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Nair GB Faruque SM Bhuiyan NA Kamruzzaman M Siddique AK Sack DA 《Journal of clinical microbiology》2002,40(9):3296-3299
The sixth pandemic of cholera and, presumably, the earlier pandemics were caused by the classical biotype of Vibrio cholerae O1, which was progressively replaced by the El Tor biotype representing the seventh cholera pandemic. Although the classical biotype of V. cholerae O1 is extinct, even in southern Bangladesh, the last of the niches where this biotype prevailed, we have identified new varieties of V. cholerae O1, of the El Tor biotype with attributes of the classical biotype, from hospitalized patients with acute diarrhea in Bangladesh. Twenty-four strains of V. cholerae O1 isolated between 1991 and 1994 from hospitalized patients with acute diarrhea in Matlab, a rural area of Bangladesh, were examined for the phenotypic and genotypic traits that distinguish the two biotypes of V. cholerae O1. Standard reference strains of V. cholerae O1 belonging to the classical and El Tor biotypes were used as controls in all of the tests. The phenotypic traits commonly used to distinguish between the El Tor and classical biotypes, including polymyxin B sensitivity, chicken cell agglutination, type of tcpA and rstR genes, and restriction patterns of conserved rRNA genes (ribotypes), differentiated the 24 strains of toxigenic V. cholerae O1 into three types designated the Matlab types. Although all of the strains belonged to ribotypes that have been previously found among El Tor vibrios, type I strains had more traits of the classical biotype while type II and III strains appeared to be more like the El Tor biotype but had some classical biotype properties. These results suggest that, although the classical and El Tor biotypes have different lineages, there are possible naturally occurring genetic hybrids between the classical and El Tor biotypes that can cause cholera and thus provide new insight into the epidemiology of cholera in Bangladesh. Furthermore, the existence of such novel strains may have implications for the development of a cholera vaccine. 相似文献