4.
Nidogen 1 (NID1) is a glycoprotein found in basement membranes involved in cross-linking collagen IV and laminin. The role of NID in breast cancer has only been evaluated in a small number of studies and the findings of these studies have been inconsistent. Our previous work revealed that highly tumorigenic murine mammary tumor cells express high levels of
Nid1 while weakly tumorigenic mammary tumor cells express low levels of
Nid1. To investigate Nid1, two stable knockdown lines were created, and
Nid1 knockdown was confirmed at both the mRNA and protein level.
Nid1 knockdown significantly reduced cell proliferation and migration/invasion and these reductions in proliferation and migration/invasion could be rescued by conditioned media containing NID1 protein. The reduced migration/invasion observed in the
Nid1 knockdown cells was not associated with significant alterations in the epithelial gene
Cdh1 or the mesenchymal genes
Snai1, Snai2, Twist1, Twist2, Zeb1 and
Zeb2. Therefore, suppression of Nid1 expression reduces proliferation and migration/invasion in claudin-low murine mammary tumor cells.
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