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Giakkoupi P Xanthaki A Kanelopoulou M Vlahaki A Miriagou V Kontou S Papafraggas E Malamou-Lada H Tzouvelekis LS Legakis NJ Vatopoulos AC 《Journal of clinical microbiology》2003,41(8):3893-3896
Seventeen Klebsiella pneumoniae clinical isolates carrying the bla(VIM-1) metallo-beta-lactamase gene were collected in the intensive care units of three hospitals in Athens, Greece, in 2002. They exhibited various carbapenem resistance levels (Etest MICs of imipenem ranged from 4 to 32 microg/ml). All isolates gave positive results by the imipenem-EDTA synergy Etest. The isolates were classified into four main types by pulsed-field gel electrophoresis; the majority of the isolates (5 and 10 isolates) belonged to two types. The bla(VIM-1) gene cassette was part of the variable region of a class 1 integron that also included aac6, dhfrI, and aadA. This structure was carried by transferable plasmids. 相似文献
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Nikolaos Athanasiou Katerina Baou Eleni Papandreou Georgia Varsou Anastasia Amfilochiou Elisavet Kontou Athanasia Pataka Konstantinos Porpodis Ioanna Tsiouprou Evangelos Kaimakamis Serafeim-Chrysovalantis Kotoulas Evgenia Katsibourlia Christina Alexopoulou Izolde Bouloukaki Meropi Panagiotarakou Aspasia Dermitzaki Nikolaos Charokopos Kyriakh Pagdatoglou Kallirroi Lamprou Sofia Pouriki Foteini Chatzivasiloglou Zoi Nouvaki Alexandra Tsirogianni Ioannis Kalomenidis Paraskevi Katsaounou Emmanouil Vagiakis 《Journal of sleep research》2023,32(1):e13656
Growing evidence suggests that sleep could affect the immunological response after vaccination. The aim of this prospective study was to investigate possible associations between regular sleep disruption and immunity response after vaccination against coronavirus disease 2019 (COVID-19). In total, 592 healthcare workers, with no previous history of COVID-19, from eight major Greek hospitals were enrolled in this study. All subjects underwent two Pfizer–BioNTech messenger ribonucleic acid (mRNA) COVID-19 vaccine BNT162b2 inoculations with an interval of 21 days between the doses. Furthermore, a questionnaire was completed 2 days after each vaccination and clinical characteristics, demographics, sleep duration, and habits were recorded. Blood samples were collected and anti-spike immunoglobulin G antibodies were measured at 20 ± 1 days after the first dose and 21 ± 2 days after the second dose. A total of 544 subjects (30% males), with median (interquartile range [IQR]) age of 46 (38–54) years and body mass index of 24·84 (22.6–28.51) kg/m2 were eligible for the study. The median (IQR) habitual duration of sleep was 6 (6–7) h/night. In all, 283 participants (52%) had a short daytime nap. In 214 (39.3%) participants the Pittsburgh Sleep Quality Index score was >5, with a higher percentage in women (74·3%, p < 0.05). Antibody levels were associated with age (r = −0.178, p < 0.001), poor sleep quality (r = −0.094, p < 0.05), insomnia (r = −0.098, p < 0.05), and nap frequency per week (r = −0.098, p < 0.05), but after adjusting for confounders, only insomnia, gender, and age were independent determinants of antibody levels. It is important to emphasise that insomnia is associated with lower antibody levels against COVID-19 after vaccination. 相似文献
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Maria Papaioannou Georgia Pitsiou Katerina Manika Paschalina Kontou Pavlos Zarogoulidis Lazaros Sichletidis 《COPD》2014,11(5):489-495
The COPD assessment test (CAT) is a short questionnaire designed to assess the impairment in health status of COPD patients. We aimed to determine the change of the CAT in COPD patients after 1 year of treatment and test the association between the score and clinical and lung function variables. Methods A cohort of 111 newly diagnosed COPD patients in primary care was evaluated at baseline and one year after the implementation of the recommended treatment according to the Global Initiative for the management of COPD (GOLD). Results Most of the patients (82%) were diagnosed with mild to moderate airflow limitation (mean FEV1 72 ± 21.5% predicted) and the CAT score increased in proportion with the GOLD stage of severity. The CAT significantly correlated with the number of exacerbations, visits to general practitioners and days of hospitalization both at the beginning and at 1 year follow-up. A strong negative correlation between the CAT score and FEV1 predicted was also observed. The CAT was responsive to the application of treatment with a significant improvement in the mean score (95% confidence interval) following 12 months of treatment by –2.4 (–2.9, –1.9) despite the small decline in lung function indices. The number of exacerbations in the preceding year and FEV1 were independent predictors of the CAT score in the general linear model. Conclusion The CAT questionnaire may serve as a simple, measurable tool complementary to spirometry in the assessment of severity and of response to treatment in unselected COPD patients in primary care. 相似文献
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W Ingram N C Lea J Cervera U Germing P Fenaux B Cassinat J J Kiladjian J Varkonyi P Antunovic N B Westwood M J Arno A Mohamedali J Gaken T Kontou B H Czepulkowski N A Twine J Tamaska J Csomer S Benedek N Gattermann E Zipperer A Giagounidis Z Garcia-Casado G Sanz G J Mufti 《Leukemia》2006,20(7):1319-1321
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Manta S Xipnitou A Kiritsis C Kantsadi AL Hayes JM Skamnaki VT Lamprakis C Kontou M Zoumpoulakis P Zographos SE Leonidas DD Komiotis D 《Chemical biology & drug design》2012,79(5):663-673
Glycogen phosphorylase is a molecular target for the design of potential hypoglycemic agents. Structure‐based design pinpointed that the 3′‐position of glucopyranose equipped with a suitable group has the potential to form interactions with enzyme’s cofactor, pyridoxal 5′‐phosphate (PLP), thus enhancing the inhibitory potency. Hence, we have investigated the binding of two ligands, 1‐(β‐d ‐glucopyranosyl)5‐fluorouracil (GlcFU) and its 3′‐CH2OH glucopyranose derivative. Both ligands were found to be low micromolar inhibitors with Ki values of 7.9 and 27.1 μm , respectively. X‐ray crystallography revealed that the 3′‐CH2OH glucopyranose substituent is indeed involved in additional molecular interactions with the PLP γ‐phosphate compared with GlcFU. However, it is 3.4 times less potent. To elucidate this discovery, docking followed by postdocking Quantum Mechanics/Molecular Mechanics – Poisson–Boltzmann Surface Area (QM/MM‐PBSA) binding affinity calculations were performed. While the docking predictions failed to reflect the kinetic results, the QM/MM‐PBSA revealed that the desolvation energy cost for binding of the 3′‐CH2OH‐substituted glucopyranose derivative out‐weigh the enthalpy gains from the extra contacts formed. The benefits of performing postdocking calculations employing a more accurate solvation model and the QM/MM‐PBSA methodology in lead optimization are therefore highlighted, specifically when the role of a highly polar/charged binding interface is significant. 相似文献
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Metabolism of galactose in the brain and liver of rats and its conversion into glutamate and other amino acids 总被引:1,自引:0,他引:1
Martin Roser Djuro Josic Maria Kontou Kurt Mosetter Peter Maurer Werner Reutter 《Journal of neural transmission (Vienna, Austria : 1996)》2009,116(2):131-139
Time- and dose-dependent measurements of metabolites of galactose (with glucose as control) in various organs of rats are
discussed. Not only the liver but especially the brain and to a lesser extent the muscles also have the capacity to take up and metabolize
galactose. Primarily, the concentrations of UDP-galactose, a pivotal compound in the metabolism of galactose, and UDP-glucose
are measured. An important feature lies in the demonstration that galactose and glucose are metabolized to amino acids and
that the only increases observed in the brain appear in the concentrations of glutamate, glutamine, GABA measured after acute
galactose loads. In addition the increase in the amino acid concentrations after galactose has been administered persists
for longer periods of time than after glucose administration. This conversion of hexoses, especially galactose, to amino acids
requires the consumption of ammonia equivalents in the brain; this finding might stimulate the use of galactose as a new means
of removal of this neurotoxic compound from the brain in patients suffering from hepatic encephalopathy or Alzheimer’s disease. 相似文献
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Maria Mavroudi Despoina Papakosta Theodore Kontakiotis Kaliopi Domvri George Kalamaras Vasiliki Zarogoulidou Paul Zarogoulidis Paschalina Latka Haidong Huang Wolfgang Hohenforst-Schmidt Konstantinos Zarogoulidis 《Sleep & breathing》2018,22(2):393-400