rhGM-CSF作为成人乙肝疫苗无(弱)应答者免疫佐剂初探

Objective To investigate the effect of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) as adjuvant on immune response in adults of non-and hyporesponders to hepatitis B vaccine. Methods Those who were once immunized with recombined yeast gene hepatitis B vaccine more than one standard scheme in two years and negative for hepatitis B markers were randomly sorted as group A and group B. 33 adults of group A were given hepatitis B vaccine 10 μg each time. The immune procedure was O, 1 and 6month. 34 adults of group B were given rhGM-CSF 300 μg for the first day, then 10 μg each time for routine immune. The blood samples were collected before the first injection and in 1, 2 and 8 months (T1, T2, TS)following the first injection to test Anti-HBs. Results Anti-HBs positive conversion rates of group A and B at T8 was 39.39% and 64.71% respectively(P=0.038). Anti-HBs levels of group B at TI, T2, T8 were(113.85±198.56) mIU/ml, (312.40±349.44) mIU/ml, (427.74±411. 58) mIU/ml (P=0.001). There was significant difference between group A and B in T8 Anti-HBs levels(P=0.010). Conclusion Better immune response was found in the group of rhGM-CSF with hepatitis B vaccine. So rhGM-CSF can induce the immune respond to hepatitis B vaccine.     

蓝氏贾第鞭毛虫承德株分子生物学研究

目的：探讨蓝氏贾第鞭毛虫大、小型包囊存在的原因。方法：用分子生物学技术对３株贾第虫（ＣＨ２、ＣＨ３、ＣＨ４）进行ｔｉｍ基因扩增和序列测定。结果：经与ＧｅｎＢａｎｋ登记虫株比较和用ＤＮＡｓｔａｒ软件处理的结果表明,此３株贾第虫的基因型属２型（ＪＨ型）。结论：大、小型包裹在形态学上经过统计学处理显示有显著差异,但与基因型无关。     

Benefiting Qi and moistening lung for arresting cough combined with Western medicine in the treatment of 56 children with bronchial asthma in remission stage

目的 观察益气润肺止咳法结合西药治疗支气管哮喘的临床疗效.方法 将56例支气管哮喘患者随机分为两组.治疗组30例,予益润肺止咳法结合西药常规治疗；对照组26例,予西药常规治疗,疗程为两周.结果 治疗组有效率为95％,对照组为80％,两组总有效率比较,差异有统计学意义(P＜0.05).结论 益气润肺止咳法结合西药治疗支气管哮喘疗效确切.     

合并神经损害的危重型手足口病患儿临床特征及预后分析

目的:分析合并神经损害的危重型手足口病的临床特征及预后影响因素,为临床诊治和早期康复提供依据。方法:收集58例合并神经损害的危重型手足口病患儿的临床资料,进行汇总分析。对存活的42例患儿进行随访。结果:58例患儿中,脑干脑炎42例(72.4%);急性弛缓性麻痹13例(22.4%),其中脑脊髓炎8例,类脊髓灰质炎综合征5例;脑炎3例(5.2%)。随访到的42例患儿中,康复30例,有后遗症12例(28.6%);肺出血、腱反射减弱和肌力0级是影响神经预后的主要危险因素。结论:危重型手足口病患儿的神经系统损害以脑干脑炎和急性弛缓性麻痹为主,其后遗症的发生率高;合并肺出血、腱反射减弱和肌力0级的患儿预后差。     

三班制连续排班对护士睡眠状况的影响

目的 了解三班制连续排班对护士睡眠状况的影响,为提高护士的睡眠质量提供依据. 方法 采用睡眠状况自评量表(SRSS)对广州地区3家三级甲等医院实行三班制连续排班后的111名护士及实行传统排班制的117名护士的睡眠状况进行调查,并进行χ2检验及t检验. 结果 三班制连续排班护士的SRSS睡眠因子总分≥23分者55名(49.5%),而传统排班制护士的SRSS睡眠因子总分≥23分者72名(61.5%),三班制连续排班护士的总均分及各因子分与传统排班制比较,除睡眠时间及觉醒不足外,均显著好于传统排班制;三班制连续排班与传统排班制护士的睡眠状况比较以20～30岁的年龄段最明显.不同职称的三班制和传统制排班的护士间睡眠障碍分布情况比较差异显著. 结论 三班制连续排班能减轻护士压力,一定程度上能改善护士的睡眠质量,特别是低年资护士的睡眠质量,从而确保护理安全.     

枳术丸加减治疗胃食管反流病的临床观察

目的：观察枳术丸加减方治疗胃食管反流病(gastro—esophaageal reflux disease,GERD)的疗效,并探讨其作用机制。方法：将63例GERD患者随机分为治疗组(30例)和对照组(33例),治疗组以枳术丸加减方治疗,每日1剂,分2次服,每次100ml,餐前0．5h服;对照组以雷尼替丁片0．15g,每天2次,西沙比利片10mg,每天3次,均餐前服,疗程均为8周。分别检测和记录两组治疗前后症状评分、内镜检查、食管压力测定、24hpH监测和胃动素,观察上述指标治疗前后两组间变化情况。结果：两组治疗后其症状积分、食管压力测定、24hDH监测和血胃动素水平均较治疗前有改善(P<0．05),且治疗组较对照组改善更为明显(P<0．05)。结论：枳术丸加减方具有较好的治疗GERD效果。     

rhGM-CSF作为成人乙肝疫苗无(弱)应答者免疫佐剂初探

Objective To investigate the effect of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) as adjuvant on immune response in adults of non-and hyporesponders to hepatitis B vaccine. Methods Those who were once immunized with recombined yeast gene hepatitis B vaccine more than one standard scheme in two years and negative for hepatitis B markers were randomly sorted as group A and group B. 33 adults of group A were given hepatitis B vaccine 10 μg each time. The immune procedure was O, 1 and 6month. 34 adults of group B were given rhGM-CSF 300 μg for the first day, then 10 μg each time for routine immune. The blood samples were collected before the first injection and in 1, 2 and 8 months (T1, T2, TS)following the first injection to test Anti-HBs. Results Anti-HBs positive conversion rates of group A and B at T8 was 39.39% and 64.71% respectively(P=0.038). Anti-HBs levels of group B at TI, T2, T8 were(113.85±198.56) mIU/ml, (312.40±349.44) mIU/ml, (427.74±411. 58) mIU/ml (P=0.001). There was significant difference between group A and B in T8 Anti-HBs levels(P=0.010). Conclusion Better immune response was found in the group of rhGM-CSF with hepatitis B vaccine. So rhGM-CSF can induce the immune respond to hepatitis B vaccine.     

东方田鼠实验室饵料筛选试验

目的寻找对东方田鼠适口性好的饵料以配制毒饵，在发现东方田鼠可能迁移入垸时进行预防性治理。方法以防治农田害鼠常用的饵料——稻谷为对照饵料，比较西瓜、西红柿、梨、苹果、马铃薯、南瓜等饵料的适口性；同时，又以西瓜、西红柿、苹果、梨作对照饵料，分别与其他瓜果饵料进行适口性比较。结果西瓜、西红柿、苹果、梨、马铃薯对东方田鼠的适口性优于稻谷，摄食系数分别为4．73、4．15、3．40、3．28、1．85，尤以前4种饵料的适口性最佳，摄食系数均在3．00以上；在以瓜果饵料为对照的试验中，也以西瓜、西红柿、苹果和梨的适口性最好，南瓜的适口性最差，摄食系数均在0．30以下。结论可用含水较高的西瓜、西红柿、梨、苹果、马铃薯作为饵料配制毒饵，防治东方田鼠，尤以前4种饵料为佳。     

运动病的神经生物学基础

运动病是伴随着运动引起的恶心、呕吐、肠胃不适、食欲低下、头痛、面色苍白和血压变化等一系列自主神经系统功能紊乱的反应症状。目前关于运动病的发病机制有多种学说,运动病发生的神经通路尚未明了,运动病的预防和治疗有待于进一步的探索。本文综述了近年来关于运动病发生机制的学说、神经通路以及预防和治疗等方面的研究进展。     

糖尿病性视网膜血管径改变

目的 量化糖尿病视网膜动静脉血流与血管横径的改变。方法 糖尿病患者和非糖尿病对照组跟底彩色照相和荧光血管造影检查，比较检查结果，并统计分析。结果 跟底荧光造影的血管横径显著大于彩照的血流横径；糖尿病组的血管横径大于对照组；颞侧的血管横径大于鼻侧；用彩色照片计算的第4分支动静脉比值大于用荧光造影结果计算。结论 颞侧分支血管横径和其轴流宽度相对大于鼻侧。与颞侧视网膜面积较大，颞侧视网膜起丰要的感光功能，新陈代谢较旺盛，需氧量增加，血流量增多有关。视网膜血管湍流表现为彩照的血流宽度等于甚至大于荧光造影的血管横径。在视网膜动脉第4分支可见血流以湍流方式流动，表明分支血管血流速度加快，或血管阻力增加。荧光造影动静脉期，静脉外周血浆层最早呈现荧光，说明在微小静脉腔内，越靠近管壁流速不是越慢，反而是最快。这种血流逆反轴流现象可解释血管口径变小反而血流阻力降低的原因。糖尿病组的静脉分支血管横径大于非糖尿病组的同级血管径，与糖尿病视刚膜组织缺血，高血糖使视网膜血管径自我调节力受损有关。对疑似高血压或动脉粥样硬化，特别是伴糖尿病者，最好应用荧光造影结果来测量动静脉血管比值，避免眼底镜检查方法判定血管动脉硬化的高假阴性率，防止漏诊。