全文获取类型
收费全文 | 596篇 |
免费 | 29篇 |
国内免费 | 4篇 |
专业分类
儿科学 | 10篇 |
妇产科学 | 9篇 |
基础医学 | 107篇 |
口腔科学 | 16篇 |
临床医学 | 56篇 |
内科学 | 113篇 |
皮肤病学 | 30篇 |
神经病学 | 34篇 |
特种医学 | 32篇 |
外科学 | 84篇 |
综合类 | 4篇 |
预防医学 | 26篇 |
眼科学 | 8篇 |
药学 | 35篇 |
中国医学 | 4篇 |
肿瘤学 | 61篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 6篇 |
2021年 | 23篇 |
2020年 | 6篇 |
2019年 | 14篇 |
2018年 | 9篇 |
2017年 | 7篇 |
2016年 | 18篇 |
2015年 | 21篇 |
2014年 | 26篇 |
2013年 | 28篇 |
2012年 | 41篇 |
2011年 | 46篇 |
2010年 | 29篇 |
2009年 | 24篇 |
2008年 | 25篇 |
2007年 | 29篇 |
2006年 | 44篇 |
2005年 | 44篇 |
2004年 | 20篇 |
2003年 | 36篇 |
2002年 | 38篇 |
2001年 | 6篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 11篇 |
1997年 | 11篇 |
1996年 | 10篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1975年 | 1篇 |
1969年 | 1篇 |
1959年 | 1篇 |
1958年 | 3篇 |
1957年 | 2篇 |
1956年 | 2篇 |
1955年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有629条查询结果,搜索用时 15 毫秒
1.
Shigeki Ohgitani Akimitsu Miyauchi Yasuyuki Takagi Yoshio Fujii Takuo Fujita Misa Nakamura Zhi-qiang Zhang Liang Shan Mitsuyo Sasaki Ryuichi Tsukino Toyoharu Yokoi Kennichi Kakudo Tomitaka Nakayama Junya Toguchida Hiroshi Kanoe Shinichi Aizawa Masao S. Sasaki Takashi Nakamura M. Suda K. Tanaka Y. Ogawa N. Tamura A. Yasoda H. Itoh M. Uehira N. Nishimoto T. Takigawa K. Shiota K. Nakao 《Journal of bone and mineral metabolism》1997,15(3):165-171
2.
3.
4.
Liang Shan Yasushi Nakamura Misa Nakamura Toyoharu Yokoi Kennichi Kakudo 《Pathology international》1998,48(8):569-574
Hyperparathyroidism refers to a term representing a wide spectrum of parathyroid disorders that are characterized by the increased production of parathyroid hormone. Hyperparathyroidism was once thought to be tare but is now more commonly recognized, aifecting 1 in 500 women over 40 years of age. Yet the interpretation of parathyroid pathology is still controversial and confusing. Over the past 10 years, genetic changes ( ret and menin genes) involved in the pathogenesis of MEN 2 and MEN 1 have been discovered in succession. Different mutations of the calcium-sensing receptor gene have been identified in neonatal severe hyperparathyroidism and familial hypocalciuric hypercal-cemia, respectively. The HRPT 2 gene responsible for the development of heredltaty hyperparathyroidism and jaw tumors has been localized on the 1q21–31 locus. Several genetic alterations have also been characterized in primary and secondary hyperparathyroidism. Different genetic alterations appear to involve the development of different types of hyperparathyroidism. These novel advances give us new insights into the pathogenesis of hyperparathyroidism and allow better differentiation between the different types of parathyroid disorders. 相似文献
5.
6.
Virpi Rantanen Seija Grénman Kaisa Kurvinen Sakari Hietanen Misa Raitanen Stina Syrjänen 《Gynecologic oncology》1998,71(3):352-358
Objective.The correlation betweenp53tumor suppressor gene mutations and the presence of high-risk human papillomavirus (HPV) DNA with thein vitroradiosensitivity of gynecological malignancies was studied in 26 cell lines derived from gynecological cancers of 23 patients.Methods.Comparison of the intrinsic radiosensitivity was performed with mean inactivation dose (D?) determined with the 96-well plate clonogenic assay.p53mutations were investigated with polymerase chain reaction and single-strand conformation polymorphism (PCR–SSCP) analysis and direct DNA sequencing, and the presence of HPV DNA was studied with PCR using HPV consensus primers.Results. p53mutations were found in 6 of 10 vulvar squamous cell carcinoma (SCC) lines. Nine vulvar and 1 vaginal SCC cell lines were HPV DNA negative and 1 vulvar cell line was HPV 16 positive. All 4 cervical SCC lines were HPV positive and possessed the wild-typep53.Three cell lines expressed HPV 16 and 1 HPV 68. Among 10 endometrial cancer cell lines, 2 cell lines with mutantp53and 1 HPV 16 positive cell line were found. No correlation could be demonstrated between inactivation of thep53gene and radiosensitivityin vitro;the cell lines were evaluated as one group or according to their anatomical origin or histology.Conclusion.Our results indicate that inactivation of thep53gene through mutation or binding with HPV DNA does not increase the resistance of gynecological malignancies to ionizing radiationin vitro. 相似文献
7.
Asuka Watanabe Misa Togi Terutsugu Koya Makoto Taniguchi Takuya Sakamoto Kuniyoshi Iwabuchi Tomohisa Kato Jr. Shigetaka Shimodaira 《Genes to cells : devoted to molecular & cellular mechanisms》2021,26(5):313-327
As the sentinels of innate and adaptive immune system, dendritic cells (DCs) have been considered to hold a great promise for medical application. Among the diverse types of DCs, monocyte-derived DCs (mo-DCs) generated in vitro have been most commonly employed. We have been improving the culture protocol and devised a protocol to produce mature interferon-α-induced DCs (IFN-DCs), hereinafter called (mat)IFN-DCs. While exploring the relationship between the expression of CD56 and the cytotoxic activity of (mat)IFN-DCs, we unexpectedly found that sorting of (mat)IFN-DCs with CD56 antibody-coated microbeads (MB) resulted in fractionating cells with tumoricidal activity into the flow-through (FT) but not MB-bound fraction. We uncovered that the FT fraction contains cells expressing low but substantial level of CD56. Moreover, those cells express granzyme B (GrB), perforin (PFN), and serpin B9 at high levels. By employing a specific inhibitor of PFN, we confirmed that direct tumoricidal activity relies on the GrB/PFN pathway. We designated subpopulation in FT fraction as CD56dim and that in CD56 positively sorted fraction as CD56bright, respectively. This is the first time, to our knowledge, to identify subpopulations of CD56-positive IFN-DCs with distinct tumoricidal activity which is ascribed to high expression of the components of GrB/PFN pathway. 相似文献
8.
Makio Kusaoi Ken Yamaji Go Murayama Misa Yasui Risa Yamada Ruka Hishinuma Takuya Nemoto Katsura Hohtatsu Michiaki Kageyama Toshio Kawamoto Kaoru Sugimoto Fumio Sekiya Takayuki Kon Michihiro Ogasawara Kazuo Kempe Hiroshi Tsuda Yoshinari Takasaki 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2012,16(5):456-466
Leukocytapheresis (LCAP) is a safe, unique therapy pertaining to intractable rheumatoid arthritis (RA) even in cases of drug allergy or infectious states. To investigate how to represent LCAP efficacy, we have conducted gene expression analyses from the peripheral blood of RA patients treated with non‐woven polyethylene terephthalate filters. Peripheral blood samples were collected immediately before and after treatment from eight RA patients who received LCAP. Among these patients, all of them achieved 20% improvement in the core set of the American College of Rheumatology (ACR20), and thus, they were confirmed as LCAP responders. Gene expression analysis was done with a high‐resolution DNA microarray. The results of each of the two groups' gene expression values (immediately before and after LCAP) were calculated using Welch's t‐test. Calculations were performed with a statistical software R.basic package: if the P‐value was less than 0.05, this was seen as a significant change. In a comparison of 25 370 gene expressions, the number of genes showing a P‐value < 0.05 in the upregulating group was 2110, and in the downregulating group it was 1864. The results of pathway analysis using the MetaCore program indicate that gene groups work for cytoskeletal remodeling are upregulated, and genes related to immune responses, such as antigens presenting via major histocompatibility complex class I and II, are downregulated just after LCAP. These findings may relate to LCAP efficacy for RA patients, but this needs further investigation. 相似文献
9.
R. Fernández‐de‐Misa B. Hernández‐Machín O. Servitje F. Valentí‐Medina L. Maroñas‐Jiménez P. L. Ortiz‐Romero J. Sánchez Schmidt R. M. Pujol F. Gallardo I. Pau‐Charles M. P. García Muret S. Pérez Gala C. Román J. Cañueto L. Blanch Rius R. Izu A. Ortiz‐Brugués R. M. Martí M. Blanes M. Morillo P. Sánchez Y. Peñate J. Bastida A. Pérez Gil I. Lopez‐Lerma C. Muniesa T. Estrach 《Clinical and experimental dermatology》2018,43(2):137-143
10.
Ayumi Hida Misa Imaizumi Benjamin French Waka Ohishi Daisuke Haruta Katsumi Eguchi Hideki Nakamura Atsushi Kawakami 《Medicine》2021,100(24)
Previous studies have suggested that human T-cell leukemia virus type 1 (HTLV-1) might act as a pathogen in rheumatoid arthritis (RA), but epidemiological evidence of an association is scarce. We measured anti-HTLV-1 antibodies among Nagasaki atomic bomb survivors to determine whether HTLV-1 is related to RA and whether radiation exposure is associated with HTLV-1 and RA prevalence.This is a cross-sectional study among atomic bomb survivors who participated in biennial health examinations from 2006 to 2010. Serum levels of anti-HTLV-1 antibodies were measured using a chemiluminescent enzyme immunoassay and confirmed by Western blotting. Association between HTLV-1 and RA was analyzed by a logistic regression model.Of 2091 participants (women 61.5%; median age, 73 years), 215 (10.3%) had anti-HTLV-1 antibodies. HTLV-1 prevalence was higher among women (13.1% vs 5.8%; P < .001). Twenty-two participants (1.1%) were diagnosed with RA. HTLV-1 prevalence among RA participants was significantly higher than that among non-RA participants (27.3% vs 10.1%; P = .020). After adjustment for age, sex, and hepatitis C virus infection, HTLV-1 was significantly associated with prevalent RA (odds ratio, 2.89; 95% confidence interval, 1.06, 7.03). There was no association between radiation dose and either the prevalence of HTLV-1 or RA.This study, among a well-defined group of atomic bomb survivors, suggests that HTLV-1 is associated with RA. 相似文献