Preparation of porous calcium phosphate microspheres with phosphate-containing molecules at room temperature for drug delivery and osteogenic differentiation

Calcium phosphate (CaP) has similar chemical properties to those of the inorganic component of human bone tissue, for potential application in drug delivery for the chemotherapy of osteosarcoma. In this work, CaP with a porous microsphere structure has been synthesized using fructose-1,6-bisphosphate (FBP) as the phosphorus source by a simple wet-chemical strategy at room temperature. The CaP porous microspheres, as an organic–inorganic hybrid nano-platform, exhibit good doxorubicin (Dox) loading capacity, and Dox-loading CaP, enhancing the in vitro chemotherapy of osteosarcoma cells. The CaP porous microspheres show high biocompatibility, and induce the osteogenic differentiation of MC3T3-E1. These results indicate that the CaP porous microspheres reported in this study are promising for application as an anti-osteosarcoma drug carrier and osteoinductive material for bone regeneration in the treatment of osteosarcoma.

Porous calcium phosphate microspheres were prepared at room temperature, and displayed potential for application in the chemotherapy of osteosarcoma and osteogenic differentiation.     

Zinc finger of the cerebellum 5 promotes colorectal cancer cell proliferation and cell cycle progression through enhanced CDK1/CDC25c signaling

IntroductionColorectal cancer (CRC), mostly caused by external or environmental factors, is the third most common and lethal cancer worldwide. Although a large number of investigations have been carried out to reveal the evolution of CRC, the underlying mechanisms of CRC remain unclear.Material and methodsExpression of zinc finger of the cerebellum 5 (ZIC5) in CRC tissues and cell models was measured by qRT-PCR and IHC. Cell transfection was carried out for ZIC5 overexpression or knockdown. The MTT assay was applied to examine the capacity of glioma cell proliferation. Wound healing assay and tumor invasion assay were used to test the capacity of glioma cell migration and invasion respectively. Cell cycle analysis and western blot were used to verify the apoptosis rates of CRC cells upon ZIC5 overexpression or downregulation. A further tumor Xenograft study was used to examine the effects of ZIC5 on tumor malignancy in vivo.ResultsCell models using HCT116 and SW620 cells were established to study the ZIC5 function upon ZIC5 overexpression of knockdown. Consistently, we discovered that ZIC5 also significantly increased in Chinese CRC patients. In addition, ZIC5 promoted CRC cell proliferation through increasing the proportion of cells maintained in the S phase. ZIC5 overexpression facilitated the capacity of CRC cell migration and invasion. Inhibition of ZIC5 mitigated such malignant effects.ConclusionsCollectively, investigations of the ZIC5 in CRC provided a new insight into CRC diagnosis, treatment, prognosis and next-step translational therapeutic developments from bench to clinic.     

钛金属内植物表面抗菌肽的应用进展

抗菌肽广泛存在于多种生物体内,是生物体非特异性免疫功能的重要组成部分。其可以保护机体免受细菌、真菌、寄生虫和病毒等病原体的侵袭,还具有介导催化、凋亡、免疫调节活性、促进伤口愈合和促成骨等作用,是钛金属内植物表面抗菌涂层研究的热点,具有良好的应用前景。本文就应用于钛金属内植物表面的抗菌肽的抗菌机制、加载方式和应用现状等进行综述。     

不同方向透明角膜小切口白内障术后对角膜散光的影响

目的探讨不同方向透明角膜小切口白内障术后对角膜散光的影响。方法我科接受透明角膜切口白内障超声乳化吸除联合人工晶状体植入术的患者共34例,按手术切口方向位于角膜曲率最大子午线方向或位于颞上方及鼻上方分为Ⅰ、Ⅱ两组,术后比较角膜散光的变化情况。结果两组患者术后随着时间延长,手术角膜散光度均逐渐减小,差异有统计学意义(P<0.05)。两组患者的角膜散光度均在术后1周时较术前增加最多,但随着时间增加,角膜散光度也逐渐减小。结论采用透明角膜切口超声乳化白内障吸除联合人工晶状体植入术进行白内障治疗前应根据患者的角膜散光情况特异性选择手术切口方向,即在角膜曲率最大处子午线方向做透明角膜切口。     

咪达唑仑片在中国维吾尔族和汉族健康人体的药动学比较

目的研究健康维吾尔族和汉族志愿者单剂量口服咪达唑仑片的药动学。方法维吾尔族、汉族健康志愿者各10名,男、女各半,单剂量口服15 mg咪达唑仑片后,用HPLC法测定咪达唑仑的血浆浓度,运用DAS 2.0程序以非室模型拟合药动学参数,并对药动学参数进行独立样本t检验和非参数Mann-Whitney U test检验,以判断药动学是否存在显著性的民族差异。结果单剂量口服15 mg咪达唑仑片后,维吾尔族志愿者的主要药动学参数分别为:ρmax(124.8±50.0)μg.L-1,tmax(0.8±0.5)h,t1/2z(1.9±0.7)h,MRT0-12 h(2.8±0.8)h,CL/F(0.9±0.4)L.h-1.kg-1,Vz/F(2.3±0.7)L.kg-1和AUC0-12 h(343.2±150.9)μg.h.L-1。汉族健康受试者的主要药动学参数分别为:ρmax(103.1±26.4)μg.L-1,tmax(1.5±0.7)h,t1/2z(3.0±0.8)h,MRT0-12 h(3.6±0.4)h,CL/F(0.7±0.2)L.h-.1kg-1,Vz/F(2.7±0.8)L.kg-1和AUC0-12 h(368.8±103.4)μg.h.L-1。经检验,维吾尔族的tmax、t1/2z和MRT0-12 h比汉族的短,差异有显著性统计学意义,其余参数的民族差异无显著性统计学意义。两个民族的部分受试者的药-时曲线有双峰。结论单剂量口服咪达唑仑片后,汉族和维吾尔族健康志愿者的药动学存在较大的个体差异,且消除速率的民族差异有显著性统计学意义,临床应用时应注意个体化给药。     

2013-2017年新疆伊犁州艾滋病母婴传播现状分析

目的 了解新疆伊犁州2013-2017年艾滋病母婴传播发生情况,探讨母婴传播的影响因素,为今后工作提供参考.方法 收集2013-2017年新疆伊犁州HIV感染孕产妇及所分娩婴幼儿的相关信息.通过描述性分析,x2检验了解HIV感染孕产妇及婴幼儿的一般特征,采用多因素logistic回归分析法,对HIV母婴传播的影响因素进...     

伴有钙化的乳腺浸润性导管癌病理特征及预后分析

目的 乳腺癌已成为世界范围内女性恶性肿瘤中最常见的死因,伴有钙化的乳腺癌占乳腺癌30％～50％.探讨伴有钙化的乳腺浸润性导管癌(invasive ductal carcinoma,IDC)的临床病理特征及预后的影响因素.方法 收集2012-06-01-2013-06-30新疆医科大学附属肿瘤医院收治的初治可手术女性乳腺癌患者307例的临床病理资料和随访资料,根据乳腺X射线图像特征分为钙化组与非钙化组,回顾性分析其临床病理特征及3年无病生存率(disease-free survival,DFS).结果 钙化组在病理类型(P＜0.001)、淋巴结转移(P=0.037)、人表皮生长因子受体2(human epidermal growth fator gene-2,HER2)过表达(P=0.005),组织学分级(P=0.043)、临床分期(P=0.021)与非钙化组比较差异有统计学意义.钙化是淋巴结转移及HER2过表达的相关风险因素.钙化组淋巴结转移的风险是非钙化组的1.736倍;钙化组HER2过表达的风险是非钙化组的2.297倍.钙化组和非钙化组3年DFS分别为87.5％和94.9％.肿瘤直径(P=0.025)和淋巴结(P=0.009)是影响乳腺IDC无病生存时间的独立预后因素.结论 钙化组乳腺IDC更具有肿瘤转移性,预后较非钙化组差.