Design of highly porous Fe3O4@reduced graphene oxide via a facile PMAA-induced assembly

Advances in the synthesis and processing of graphene-based materials have presented the opportunity to design novel lithium-ion battery (LIB) anode materials that can meet the power requirements of next-generation power devices. In this work, a poly(methacrylic acid) (PMAA)-induced self-assembly process was used to design super-mesoporous Fe₃O₄ and reduced-graphene-oxide (Fe₃O₄@RGO) anode materials. We demonstrate the relationship between the media pH and Fe₃O₄@RGO nanostructure, in terms of dispersion state of PMAA-stabilized Fe₃O₄@GO sheets at different surrounding pH values, and porosity of the resulted Fe₃O₄@RGO anode. The anode shows a high surface area of 338.8 m² g⁻¹ with a large amount of 10–40 nm mesopores, which facilitates the kinetics of Li-ions and electrons, and improves electrode durability. As a result, Fe₃O₄@RGO delivers high specific-charge capacities of 740 mA h g⁻¹ to 200 mA h g⁻¹ at various current densities of 0.5 A g⁻¹ to 10 A g⁻¹, and an excellent capacity-retention capability even after long-term charge–discharge cycles. The PMAA-induced assembly method addresses the issue of poor dispersion of Fe₃O₄-coated graphene materials—which is a major impediment in the synthesis process—and provides a facile synthetic pathway for depositing Fe₃O₄ and other metal oxide nanoparticles on highly porous RGO.

Advances in the synthesis and processing of graphene-based materials have presented the opportunity to design novel lithium-ion battery (LIB) anode materials that can meet the power requirements of next-generation power devices.     

Early detection of pancreatic cancers in liquid biopsies by ultrasensitive fluorescence nanobiosensors

Numerous proteases, such as matrix metalloproteinases (MMPs), cathepsins (CTS), and urokinase plasminogen activator (UpA), are dysfunctional (that is, over- or under-expressed) in solid tumors, when compared to healthy human subjects. This offers the opportunity to detect early tumors by liquid biopsies. This approach is of particular advantage for the early detection of pancreatic cancer, which is a “silent killer”. We have developed fluorescence nanobiosensors for ultrasensitive (sub-femtomolar) arginase and protease detection, consisting of water-dispersible Fe/Fe₃O₄ core/shell nanoparticles and two tethered fluorescent dyes: TCPP (Tetrakis(4-carboxyphenyl)porphyrin) and cyanine 5.5. Upon posttranslational modification or enzymatic cleavage, the fluorescence of TCPP increases, which enables the detection of proteases at sub-femtomolar activities utilizing conventional plate readers. We have identified an enzymatic signature for the detection of pancreatic adenocarcinomas in serum, consisting of arginase, matrix metalloproteinase-1, -3, and -?9, cathepsin-B and -E, urokinase plasminogen activator, and neutrophil elastase, which is a potential game-changer.     

Peptide nanosponges designed for rapid uptake by leukocytes and neural stem cells

The structure of novel binary nanosponges consisting of (cholesterol-(K/D)_nDEVDGC)₃-trimaleimide units possessing a trigonal maleimide linker, to which either lysine (K)₂₀ or aspartic acid (D)₂₀ are tethered, has been elucidated by means of TEM. A high degree of agreement between these findings and structure predictions through explicit solvent and then coarse-grained molecular dynamics (MD) simulations has been found. Based on the nanosponges'' structure and dynamics, caspase-6 mediated release of the model drug 5(6)-carboxyfluorescein has been demonstrated. Furthermore, the binary (DK20) nanosponges have been found to be virtually non-toxic in cultures of neural progenitor cells. It is of a special importance for the future development of cell-based therapies that DK20 nanosponges were taken up efficiently by leucocytes (WBC) in peripheral blood within 3 h of exposure. The percentage of live cells among the WBC was not significantly decreased by the DK20 nanosponges. In contrast to stem cell or leucocyte cell cultures, which have to be matched to the patient, autologous cells are optimal for cell-mediated therapy. Therefore, the nanosponges hold great promise for effective cell-based tumor targeting.

Nanosponges for drug delivery.