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排序方式: 共有419条查询结果,搜索用时 31 毫秒
1.
Tadashi Kano MD Toshiro Koga Kuniyasu Souda Yoshishige Abe Tomohiro Yonemura Naokata Oka Kiyoshi Inokuchi 《Surgery today》1987,17(4):269-275
The usefulness of carcinoembryonic antigen (CEA) as an indicator for recurrence and a guide to the treatment was evaluated
from a retrospective analysis of 88 patients with recurrent gastric cancer. Sixty-two of these patients (70.5 per cent), 25
of whom had a preoperative positive assay, and 37 a negative assay, had elevated levels of CEA after disease progression.
Averaged CEA level in patients with liver metastasis was significantly higher (872 ng/ml) than in those with peritoneal metastasis
(68 ng/ml), with lymph node metastasis (103 ng/ml) or with local metastasis (93 ng/ml) (p<0.01). An elevation of CEA was found
prior to the clinical manifestation of recurrence, and the average lead time was 4 months. In 25 patients with a lead time
of more than 4 months, survival time after CEA elevation was 13.3 months, which was longer than the 6.5 months of 28 patients
with less than 4 months. Thirty-seven of the 88 patients were treated after recurrence. The average survival period after
the detection of recurrence was 9.4 months in patients with surgical treatments followed by chemotherapy, 5.9 months in those
with chemotherapy alone and 3.8 months in those with surgery alone. The average survival period of 26 patients with positive
CEA assays in recurrence was 5.1 months longer than of patients with negative assays. This fact suggested that early detection
of recurrence followed by various treatments, in the elevated CEA group, contributes to favorable results. 相似文献
2.
Kuniyasu H Yasui W Shinohara H Yano S Ellis LM Wilson MR Bucana CD Rikita T Tahara E Fidler IJ 《The American journal of pathology》2000,157(5):1523-1535
We determined whether hyperplastic mucosa adjacent to colon cancer contributes to neoplastic angiogenesis. Surgical specimens of human colon cancer (40 Dukes' stage B and 34 Dukes' stage C) were analyzed by immunohistochemistry for expression of proliferative and angiogenic molecules. The mucosa adjacent to Dukes' stage C tumors (but not Dukes' stage B tumors) had a higher Ki-67 labeling index and a higher expression of epidermal growth factor receptor and transforming growth factor-alpha than distant mucosa. The expression levels of vascular endothelial growth factor, basic fibroblast growth factor, interleukin-8, and the vascular density in the adjacent mucosa were similar to those in the tumor lesions and significantly higher than those in the distant mucosa. The expression of interferon-beta inversely correlated with the level of pro-angiogenic molecules and the vascular density. The injection of metastatic human colon cancer cells and murine colon cancer cells into the cecal wall of mice induced hyperplastic changes in the adjacent mucosa which expressed higher levels of epidermal growth factor receptor, basic fibroblast growth factor, and vascular endothelial growth factor, and lower levels of interferon-beta than did the control mucosa, which directly correlated with the degree of hyperplasia. These data suggest that metastatic human colon cancer cells can induce hyperplasia in the adjacent mucosa, which in turn produces angiogenic molecules that contribute to neoplastic angiogenesis. 相似文献
3.
Wataru Yasui Zhong-Qiang Ji Hiroki Kuniyasu Ayse Ayhan Hiroshi Yokozaki Hisao Ito Eiichi Tahara 《Virchows Archiv : an international journal of pathology》1992,421(6):513-519
Summary The expression of transforming growth factor alpha (TGF-) was examined in various human tissues and the fetus, using immunohistochemistry and Northern blot analysis. TGF- immunoreactivity was detected mainly in the epithelial cells of the digestive tract, liver, pancreas, kidney, thyroid, adrenal, skin, mammary gland and genital organs. In the digestive tract, epithelial cells with regenerative change or hyperplastic change showed strong immunoreactivity to TGF-. Peripheral nerve, vessels, megakaryocytes and macrophages in the lung and spleen were also positive for TGF-. By Northern blot analysis the expression of TGF- mRNA was confirmed in the digestive tract, salivary gland, thyroid, kidney and mammary gland. In the human fetus, the nerve tissues, liver, adrenal and kidney were positive for TGF-. Strong immunoreactivity to TGF- was observed in the hepatocytes of the fetus. These findings indicate that TGF- is produced by a variety of nonneoplastic cells in both adult and fetal tissues. 相似文献
4.
Ken Hayashi Hiroki Kuniyasu Naohide Oue Hideo Shigeishi Kazuya Kuraoka Hirofumi Nakayama Wataru Yasui 《Pathobiology》2002,70(1):40-46
DNA damage triggers the activation of checkpoints that delay cell cycle progression to allow for DNA repair. Loss of G2 checkpoints provides a growth advantage for tumor cells undergoing aberrant mitosis. However, the precise mechanisms of G2 checkpoints acting in gastric cancer are unknown. Here, we analyzed the G2 checkpoint function in two gastric cancer cells, MKN-28 cells containing a mutant p53 gene and MKN-45 cells which have wild-type p53. Two agents damaging DNA, camptothecin (CPT) or ultraviolet light (UV), were utilized to trigger a G2 phase cell cycle checkpoint response in these tumor cells. Both CPT and UV inhibited the growth of MKN-45 cells, whereas they did not affect the growth of MKN-28 cells. CPT induced cell cycle arrest at the G2/M phase and enhanced the expression of human RAD9 (hRAD9) in MKN-45 cells. In addition, hRAD9 showed perinuclear staining and similar localization with Bcl-2 in MKN-45 cells but not in MKN-28 cells after having applied CPT or UV light. These results suggest that besides p53 activity, the induction of hRAD9 is required for G2/M checkpoint signal transduction in gastric cancer cells. 相似文献
5.
Takahashi T; Kuniyasu Y; Toda M; Sakaguchi N; Itoh M; Iwata M; Shimizu J; Sakaguchi S 《International immunology》1998,10(12):1969-1980
Elimination of CD25+ T cells, which constitute 5-10% of peripheral CD4+ T
cells in normal naive mice, leads to spontaneous development of various
autoimmune diseases. These immunoregulatory CD25+CD4+ T cells are naturally
unresponsive (anergic) in vitro to TCR stimulation, and, upon stimulation,
suppress proliferation of CD25-CD4+ T cells and CD8+ T cells. The antigen
concentration required for stimulating CD25+CD4+ T cells to exert
suppression is much lower than that required for stimulating CD25-CD4+ T
cells to proliferate. The suppression, which results in reduced IL-2
production by CD25-CD4+ T cells, is dependent on cellular interactions on
antigen-presenting cells (and not mediated by far-reaching or long-lasting
humoral factors or apoptosis-inducing signals) and antigen non-specific in
its effector phase. Addition of high doses of IL-2 or anti-CD28 antibody to
the in vitro T cell stimulation culture not only breaks the anergic state
of CD25+CD4+ T cells, but also abrogates their suppressive activity
simultaneously. Importantly, the anergic/suppressive state of CD25+CD4+ T
cells appeared to be their basal default condition, since removal of IL-2
or anti-CD28 antibody from the culture milieu allows them to revert to the
original anergic/suppressive state. Furthermore, transfer of such
anergy/suppression-broken T cells from normal mice produces various
autoimmune diseases in syngeneic athymic nude mice. These results taken
together indicate that one aspect of immunologic self-tolerance is
maintained by this unique CD25+CD4+ naturally anergic/suppressive T cell
population and its functional abnormality directly leads to the development
of autoimmune disease.
相似文献
6.
Yoshikazu Noguchi Tatsuo Makino Takaki Yoshikawa Katsutoshi Nomura Kuniyasu Fukuzawa Akihiko Matsumoto Takuko Yamada 《Surgery today》1996,26(1):36-41
This study was conducted to investigate the role of tumor necrosis factor- (TNF-) and interleukin-2 (IL-2) in inducing cancer cachexia, and the results were compared with those obtained from our previous study on Fisher 344 rats with methylcholanthrene-induced sarcoma. Three groups of male Fisher 344 rats received one of the following regimens: 4×104 IU of human recombinant TNF- per rat per day subcutaneously (sc) for 5 consecutive days (n=5), 3.5×105 U human recombinant IL-2 per rat per day sc for 14 consecutive days (n=5), or normal saline (n=5). The activities of both phosphoenolpyruvate carboxykinase (PEPCK) and malic enzyme (ME) were increased slightly in the IL-2 group. Furthermore, LPL activity was significantly increased in the adipose tissue of the TNF group and in the cardiac muscle of the IL-2 group, but not in that of the TNF group. These results show that there is a significant difference between the metabolic alterations seen in the tumor-bearing state and those induced by either TNF- or IL-2 alone. Thus, it is unlikely that IL-2 or TNF- is the sole mediator of cancer cachexia in this tumor and rat model. 相似文献
7.
Hirohito Yano Takashi Funakoshi Jun Shinoda Noboru Sakai George Kokuzawa Kuniyasu Shimokawa 《Brain tumor pathology》1997,14(1):75-78
A 35-year-old woman had an intradural tumor in the posterior fossa adjacent to the posterior wall of the left pyramidal bone,
which was totally removed and histologically diagnosed as a pleomorphic adenoma. Follow-up examination for 2 years showed
no recurrence of the tumor. There was no primary lesion in any other gland of the body, and therefore there is no alternative
but to conclude a “migration” of some gland cells. The pathogenesis of this tumor remains unclassified. 相似文献
8.
9.
BACKGROUND/AIMS: The E-cadherin-mediated cell adhesion system is now considered to be an "invasion suppressor system" in cancer cells. The purpose of this study is to examine the effect of E-cadherin on morphogenesis of MKN28 human gastric carcinoma cell line in the course of E-cadherin antisense S-oligodeoxynucleotide (ODN) treatment. METHODOLOGY: The effect of E-cadherin antisense or random S-ODN treatment on cell growth, morphology in monolayer culture, and E-cadherin protein expression of MKN28 cells were evaluated. Further, immunohistochemical examination was performed. RESULTS: Cell growth under 3-microM and 6-microM E-cadherin antisense S-ODN treatment did not differ from that under random S-ODN treatment. Although the expression of E-cadherin was decreased assuredly at the time of 6 days after 3-microM E-cadherin antisense S-ODN treatment by immunohistochemical examination, cell-cell adhesion was still observed until Day 10 after the treatment. On Day 15, the cells lost the cell-cell adhesion and showed the detachment and intercellular slits at least. Expression of the insoluble fraction of E-cadherin protein decreased in E-cadherin antisense S-ODN treatment cells at 6 days. CONCLUSIONS: In this study, we demonstrated that discrepancy between E-cadherin protein expression and morphology exists in MKN28 cells treated with E-cadherin antisense S-ODN treatment. 相似文献
10.
p53 point mutations in primary human gastric carcinomas 总被引:12,自引:0,他引:12
Hiroshi Yokozaki Hiroki Kuniyasu Yasuhiko Kitadai Kenji Nishimura Hiroko Todo Ayşe Ayhan Wataru Yasui Hisao Ito Eiichi Tahara 《Journal of cancer research and clinical oncology》1992,119(2):67-70
Summary p53 point mutations in primary gastric carcinomas were analyzed by performing cDNA deoxynucleotide sequencing of the gene. Out of 16,9 (56.3%) primary gastric carcinoma cases, including early cancer, showed one or more p53 point mutations in their open-reading frame, and 4 out of 9 cases had a p53 point mutation within highly conserved domains. The characteristics of the p53 mutation spectrum observed in primary tumors were (a) frequent mutation at an A:T pair (50%, 7 out of 14 mutations), (b) high transversion incidence (29%, 4 out of 14 mutations), (c) no transition at CpG, and (d) no G:C to T:A transversion. Our results suggest that p53 mutation is a common event in gastric carcinoma occurring from the early stage of progression with its specific mutation spectrum.Abbreviation PCR-SSCP
polymerase chain reaction single-strand conformation polymorphism 相似文献