BCAN基因在肾透明细胞癌中的表达及临床意义

目的以基因表达数据集资料为研究对象,分析BCAN基因在肾透明细胞癌中的表达情况以及对患者预后的影响。方法在Oncomine数据库中挖掘BCAN在肾透明细胞癌(ccRCC)中的表达情况。从TCGA数据库中获取ccRCC患者临床资料和目的基因的表达信息并进行统计分析。利用GEO数据库中GSE73731数据集的ccRCC样本进行基因富集分析。利用String数据库分析与BCAN相关的蛋白。结果BCAN低表达组的ccRCC患者在病理分期及T分期方面低于高表达组(P<0.001;P=0.001);N分期及M分期差异无统计学意义(P>0.05)。BCAN低表达组患者的总生存期优于高表达组(P=0.033)。BCAN基因高表达组的样本主要富集在KRAS信号通路。结论BCAN可以通过多种途径来促进肿瘤细胞的侵袭能力,有望成为ccRCC不良预后的重要生物标志物之一。     

Mortality,length of stay and costs associated with acute graft-versus-host disease during hospitalization for allogeneic hematopoietic stem cell transplantation

Objective: Acute graft-versus-host disease (aGVHD) is a common and life-threatening complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The extent to which aGVHD increases inpatient costs associated with allo-HSCT has not been thoroughly evaluated. In this analysis, mortality, hospital length of stay (LOS) and costs associated with aGVHD during allo-HSCT admissions are evaluated.

Methods: This is a retrospective analysis of discharge records from the National Inpatient Sample database for patients receiving allo-HSCT between 1 January 2009 and 31 December 2013. Allo-HSCT discharges with an aGVHD diagnosis were included in the aGVHD group and those without any graft-versus-host disease (GVHD) diagnosis comprised the non-GVHD group. Mortality, LOS and costs were compared between the two groups, as well as within subgroups, including age (<18 vs. ≥18 years) and survival status (alive vs. deceased) at discharge.

Results: Overall, mortality (16.2% vs. 5.3%; p?<?.01), median hospital LOS (42.0 vs. 26.0 days; p?<?.01) and median total costs ($173,144 vs. $98,982; p?<?.01) were significantly increased in patients with aGVHD versus those without GVHD during hospitalizations for allo-HSCT, irrespective of age group. Patients with aGVHD who were <18 years of age had a lower mortality rate but greater hospital LOS and total costs versus patients aged ≥18 years. Patients who died during allo-HSCT hospitalization had longer LOS and incurred greater costs than those who survived in both the aGVHD and non-GVHD groups.

Conclusion: Occurrence of aGVHD during allo-HSCT admissions resulted in a tripling of the mortality rate and a near doubling of hospital LOS and total costs. In addition, death during allo-HSCT hospitalizations was associated with greater healthcare utilization and costs. Effectively mitigating aGVHD may improve survival and substantially reduce hospital LOS and costs for allo-HSCT.     

当归对肺纤维化大鼠肺形态学及胶原的影响

目的　观察当归对博莱霉素所致的肺间质纤维化大鼠的肺形态学及胶原的影响 ,探讨其作用机制。方法　 36只大鼠随机分为对照组、模型组及当归组。气管内注入博莱霉素复制大鼠肺间质纤维化模型 ,次日当归组每日腹腔内注射当归注射液 15 0mg/kg,于第 7及第 2 8天每组分别处死 6只大鼠 ,观察肺间质纤维化形成的病理变化及血清Ⅰ型胶原的含量。结果　当归能明显减轻肺泡炎和肺纤维化程度 ,与模型组比较 ,当归组血清Ⅰ型胶原含量于第 7、 2 8天明显降低 (P均 <0 0 5 )。结论　当归能减慢肺间质纤维化的进程 ,对肺间质纤维化有一定的保护作用。     

创伤性泌尿系出血的急诊动脉栓塞治疗

目的探讨经导管选择性动脉栓塞在创伤性泌尿系出血急诊治疗中的价值. 方法 1998年1月～2003年6月对27例创伤性泌尿系出血根据损伤出血部位行数字减影血管造影检查,明确出血动脉,明胶海绵颗粒或弹簧钢圈栓塞靶动脉. 结果 23例一次栓塞成功止血,2例2次栓塞后止血,2例栓塞失败改行其他治疗方法.栓塞后不良反应为发热16例,局部疼痛、恶心、呕吐14例,1例髂内动脉栓塞出现一过性臀部疼痛. 结论选择性动脉栓塞是治疗创伤性泌尿系出血的有效措施,具有创伤小、见效快的优点,为进一步治疗创造条件.     

Biomechanical Properties of Esophagus during Systemic Treatment with Epidermal Growth Factor in Rats

The epidermal growth factor (EGF) is a growth factor with effects on many cell types and tissue. Morphometric and passive biomechanical properties were studied in isolated segments of the esophagus in 22 EGF-treated rats and 12 control rats. The rats were divided into groups with EGF treatment for 2, 4, 7, and 14 days (n=6 for each group except n=4 for the 14 days EGF-treatment group) or saline treatment (n=3 for each group). The mechanical test was performed as a distension experiment in vitro where the whole esophagus was stretched to its in situ length and distended with pressures up to 10 cm H₂O using a ramp distension protocol. The pressure and outer diameter were recorded. Circumferential stress (force per area) and strain (deformation) were computed from the diameter and pressure data using the zero-stress state as reference. The zero-stress state was obtained by cutting esophageal rings radially. This caused the rings to open up into a sector. EGF induced pronounced morphometric changes, e.g., the wall thickness, wall cross-sectional area, and inner and outer circumferential lengths significantly increased during the EGF treatment. Histological analysis showed mucosa and submucosa growth during EGF treatment. The opening angle and residual strains increased with the highest value in the 14 days EGF-treated group (P < 0.05). The change in opening angle depended largely on the change in mucosa thickness. Furthermore, the circumferential stiffness of the esophagus reached a maximum after 7 days EGF treatment (P < 0.01). © 2003 Biomedical Engineering Society. PAC2003: 8719Rr, 8717Ee     

高原军人睡眠质量与生存质量相关性

目的了解高原军人的睡眠质量及其与生存质量的关系。方法采用随机整群抽样法,选择驻西藏某部队官兵490名,应用匹茨堡睡眠质量指数量表(PSQI)和世界卫生组织生存质量测定量表简表(WHOQOL-BREF)进行问卷调查。结果高原军人PSQI总分为(7.14±3.42),14.8%的人睡眠质量较差;睡眠问题主要表现在入睡时间、日间功能障碍和主观睡眠质量3个成分的得分较高,2分以上所占的百分比分别为56.5%,54.8%和36.9%;不同睡眠质量的高原军人在生存质量4个领域得分的差异均有统计学意义(P<0.001)。高原军人睡眠质量各成分得分和总分与生存质量4个领域的得分绝大多数呈显著负相关。结论高原军人的睡眠质量需要改善,提高高原军人睡眠质量是改善他们生存质量状况的有效途径。     

外放疗后鼻咽癌残存灶近距离治疗的近期疗效

 自1992年7月至1995年7月，我们对鼻咽癌首程治疗后，鼻咽腔内仍有残存病灶者35例采用近距离治疗补充照射，参考点放射剂量4～6Gy／次，2次／周，共2～4次，近距离治疗总剂量12～18Gy。结果显示1、3年肿瘤局部控制率分别为94．3％、73．3％，近期疗效满意，尤以T_1-2期病例为优。随诊期内未见严重放射性并发症。     

医学教学动画的创作与应用

本研究结合生理学课程,对动画应用教学进行问卷调查,分析网络下载版医学教学动画应用现状;并独立创作生理学相关动画,对教学动画应用在自主学习环节中的效果做了分析。结果显示,在自主学习环节中获得文字和教学动画资料的学生测试成绩,高于只获得文字资料的学生（P<0.01）;“完成学习内容>75%”的人数与比例方面,前者也高于后者。表明教学动画在提高学生自主学习成绩的同时,还提高了其自学兴趣。