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排序方式: 共有148条查询结果,搜索用时 15 毫秒
1.
JOHANNES DIETL ADAM CZUPPON LILO METTLER 《American journal of reproductive immunology (New York, N.Y. : 1989)》1983,4(3):116-121
ABSTRACT: In the present work, 500 and 50,000 porcine zonae pellucidae were solubilized using Lithium-3,5-diiodosalicylate. The zona antigens were purified by immunoaffinity chromatography (IAC) on immobilized antizona immunoglobulin G (IgG). The antizona-IgG was raised by immunization of female rabbits with 500 heat-solubilized porcine zonae. Four antigens could be detected following IAC: ZP I/1 (Mr = 42,000), ZP II/1 (Mr = 67,000), ZP II/2 (Mr = 32,000), ZP III/1 (Mr = 17,000). In a parallel experiment, 50,000 zonae were solubilized in a similar manner and the mixture was analyzed by high-pressure liquid chromatography (HPLC) using a protein column. Altogether, 9 protein peaks that contained the antigens ZP I/1, ZP II/1, ZP II/2, and ZP III/1 could be detected following HPLC. The carbohydrate composition is characteristic for O-glycosidic-glycoproteins. ZP II/1 and ZP II/2 are probably in close association within the zona. Based on the reaction of the antigens with antibodies induced by intact and heat-solubilized zonae, it is postulated that only ZP I/1 and ZP II/l are expressed on the surface in intact zonae. 相似文献
2.
HAKANSON ERIK; RUTBERG HANS; JORFELDT LENNART; MARTENSSON JOHANNES 《British journal of anaesthesia》1985,57(4):394-399
The influence of thoracic extradural local anaesthetics (0.5%bupivacaine) or extradural morphine on the metabolic responseto upper abdominal surgery was compared with the administrationof morphine i.v. in the period after operation. The extradurallocal anaesthetic group had significantly lower blood glucoseand plasma FFA concentrations and consistently, but not significantly,lower blood glycerol and lactate concentrations than both theother groups. At 4 h both extradural groups had significantlylower plasma FFA and blood 3-hydroxybutyrate concentrationsthan the control group. Blood alanine concentration decreasedin all three groups with a minimum at 24 h. There were no differencesin serum insulin concentrations between the groups. It is concludedthat thoracic extradural morphine differs from thoracic extradurallocal anaesthetics in being much less able to suppress the metabolicresponse associated with upper abdominal surgery. 相似文献
3.
Agnes Van Der Heide Carol Ann Remme Dirk M. Hofman JOHANNES W. G. Jacobs JOHANNES W. J. Bijlsma 《Arthritis \u0026amp; Rheumatology》1995,38(10):1466-1474
Objective. To investigate the extent to which early radiologic damage is predicted by joint inflammation in patients with newly diagnosed rheumatoid arthritis (RA). Methods. Regression analysis was performed on 1-year progression of total radiologic damage for baseline characteristics and cumulative disease activity measures, and the effects of continued joint inflammation on the progression of damage in separate joint groups were investigated. Results. Odds ratios for progression of total damage were 12 for the presence of rheumatoid factor, 5 for the presence of damage at baseline, and 2 for cumulative joint inflammation. A positive association between continued joint inflammation and progression of damage was found to be statistically significant for most joint groups. Conclusion. Progression of radiologic damage in patients with newly diagnosed RA is independently associated with the presence of rheumatoid factor and damage at baseline and with cumulative joint inflammation. 相似文献
4.
5.
A pilot study on the efficiency of treatment with 8-methoxypsoralen and longwave ultraviolet light (PUVA) on three patients with allergic contact dermatitis to nickel sulphate, potassium dichromate and to thiuram mix has been performed. Both the contact allergic reactions and the irritative skin reactions induced by dimethyl sulfoxide (DMSO) and tetrahydrofurfuryl nicotinate (Trafuril) were evaluated before and after PUVA. The total UV-A doses were respectively 69–45 and 76J/cm2. These doses induced complete clearance of the eczematous lesions in two cases; the third case showed considerable improvement. In contrast to the pronounced skin reactions seen prior to PUVA, the allergic and irritative reactions evaluated after PUVA were decreased or negative. 相似文献
6.
ARTHUR M. FELIX EDGAR P. HEIMER CHING-TSO WANG THEODORE J. LAMBROS JOSEPH SWISTOK MARTIN ROSZKOWSKI MUSTAQ AHMAD DIANNE CONFALONE JOHN W. SCOTT DAVID PARKER JOHANNES MEIENHOFER ARNOLD TRZECIAK DIETER GILLESSEN 《Chemical biology & drug design》1985,26(2):130-148
A novel synthesis of thymosin α1 by classical methods using seven tert. -butyl side chain protected fragments is described. Optimum conditions were found for the final DCC/HOBt coupling of the two key intermediates; decapeptide and octadecapeptide. Thymosin α1 was purified by two stages of preparative HPLC (partial purification with C8 and final purification with C18 reverse phase silica gel) to give a 30% overall yield for the final four stages of synthesis (including catalytic hydrogenation of octadecapeptide, coupling, deprotection and purification). The product was shown to be homogeneous by thin-layer and paper high voltage electrophoresis, isoelectric focusing analysis, thin-layer chromatography and high performance liquid chromatography. Amino acid analysis, optical rotation, 1 H-n.m.r. spectroscopy, FAB mass spectroscopy and peptide mapping after tryptic digestion confirmed the structure of thymosin α1. Three minor stereoisomer contaminants were isolated by HPLC and characterized as [D-Lys14]-thymosin α1, [D-Lys17]-thymosin α1 and [D-Ala3]-thymosin α1 resulting from racemization at Lys14, Lys17 and Ala3 during the coupling of the fragments. A final contaminant, isolated by HPLC, was characterized as Nα-isobutyloxycarbonyl-thymosin α1 (15–28), which results from “wrong way opening” of an activated mixed anhydride. 相似文献
7.
JOHANNES SPERZEL LUDWIG BINNER† GIUSEPPE BORIANI‡ MAURO BIFFI‡ JEFF SNELL§ JÖRG SCHEINER¶ EULJOON PARK§ GENE BORNZIN§ 《Pacing and clinical electrophysiology : PACE》2005,28(S1):S57-S62
AutoCapture™ based on the evoked response can be confounded by electrode polarization. In this study, polarization was measured in human subjects who had chronic atrial leads. The aim of the study was to determine whether electrode polarization can be measured using a time integral atrial evoked-response integral (AERI) of the negative portion of the atrial paced ER evoked-response signal and to determine whether high-polarization atrial leads unsuitable for AutoCapture™ can be identified a priori. Atrial intracardiac-electrogram (IEGM) signals from 39 patients with implanted pacemakers were recorded and analyzed. The signals were recorded during conventional atrial-threshold searches. A total of 221 atrial-capture thresholds were recorded, ranging from 0.25 to 2.75 V with a mean of 0.79 V. Each evoked response was evaluated using the AERI in a 36 ms window following the 0.4 ms atrial stimulus. The polarization was estimated as a linear function of stimulus voltage using the evoked-response signal integral of captured beats identified on the IEGM. The 221 threshold-search datasets were obtained using leads with eight different electrode materials. Polarization could be measured using AERI as a function of stimulus voltage. Furthermore, this polarization measure can be used to identify high-polarization leads, which are ill suited for the atrial AutoCapture™ algorithm. 相似文献
8.
MEREDITH I. SEDNEY ERIC WEIJERS ERNST E. VAN DER WALL JEEEREY D. ADIPRANOTO JAN CAMPS JACOBUS A.K. BLOKLAND ERNEST K.J. PAUWELS JOHANNES J. SCHIPPERHEIJN BEERT BUIS ALBERT V.G. BRUSCHKE 《Pacing and clinical electrophysiology : PACE》1989,12(12):1863-1868
To evaluate the adaptation of the heart to exercise during pacing, 15 patients with permanent endocardial pacemakers were studied; nine patients had atrioventricular universal (DDD) pacemakers (Symbios 7005) and six patients had activity detecting rate-responsive ventricular (VVIR) pacemakers (Activitrax 8403). Left ventricular function in each patient during rate variable pacing was compared to ventricular function during VVI single-rate pacing. End-systolic and end-diastolic volume changes during exercise were measured by radionuclide angiography and the amount of volume change was used to assess left ventricular function. Both short-term (within 4 hours) and long-term measurements (after at least 4 weeks) were made at rest and at 50% of the maximal exercise capacity in DDD or VVIR mode and were compared with VVI single-rate pacing. All patients, when changed from DDD or VVIR mode to VVI single-rate pacing showed a significant increase of the end-diastolic volume during exercise, which increased even more after long-term VVI pacing. During long-term rate variable pacing, there was no increase of the end-diastolic volume during exercise. DDD or VVIR pacing initially showed a substantial increase of the end-systolic volume during exercise combined with a decrease of left ventricular ejection fraction, suggesting a decrease of the left ventricular contractility. After 4 weeks, contractility improved both with DDD and VVIR pacing. We conclude that short-term DDD and VVIR pacing induces a temporary impairment of left ventricular function that improves after 4 weeks, whereas long-term VVI pacing is associated with left ventricular dilatation even at moderate levels of exercise. 相似文献
9.
DEBRA E. WEESE-MAYER JEAN M. SILVESTRI ANNA S. KENNY MICHEL N. ILBAWI SUSAN A. HAUPTMAN JACK W. LIPTON PASI P. TALONEN HONESTO GARRIDO GARCIA JOHN W. WATT GERHARD EXNER GERHARD A. BAER JOHN A. ELEFTERIADES WILLIAM T. PERUZZI CHARLES G. ALEX RICHARD HARLID WOLTER VINCKEN G. MICHAEL DAVIS MARC DECRAMER CHRISTOPH KUENZLE ARNE SÆTERHAUG JOHANNES G. SCHÖBER 《Pacing and clinical electrophysiology : PACE》1996,19(9):1311-1319
We sought to determine the international experience with the quadripolar diaphragm pacer system and to test two hypotheses: the incidence of pacer complications would be (1) increased among pediatric as compared to adult patients; and (2) highest among active pediatric patients with idiopathic congenital central hypoventilation syndrome (CCHS). Data were collected via a questionnaire coupled with the Atrotech Registry data for a total of 64 patients (35 children and 29 adults) from 14 countries. Thoracic implantation of electrodes and bilateral pacer use each occurred in 94% of all subjects. Tetraplegic (vs pediatric CCHS) patients were more typically paced 24 hours/day (P = 0.001). Pacing duration averaged 2.0 ± 1.0 years among children and 2.2 ± 1.1 years among adults. Infections occurred among 2.9% of surgical procedures, all in pediatric CCHS patients (vs pediatric tetraplegic patients, P = 0.01). The incidence of mechanical trauma was 3.8%, without significant differences among patient groups. The incidence of presumed electrode and receiver failure were 3.1% and 5.9%, respectively, with internal component failure greater among pediatric CCHS than pediatric tetraplegic patients (P < 0.01). Intermittent or absent function of 0–4 electrode combinations occurred among 19% of all patients, with increased frequency among pediatric CCHS than pediatric tetraplegic patients (P < 0.03). Complication- free successful pacing occurred in 60% of pediatric and 52% of adult patients. In all, 94 % of the pediatric and 86% of the adult patients paced successfully after the necessary intervention. Although pacer complications were not increased among pediatric as compared to adult patients, the incidence of complications was highest among the active pediatric patients with CCHS. Longitudinal study of these patients will provide invaluable information for modification and improvement of the quadripolar system. 相似文献