全文获取类型
收费全文 | 440篇 |
免费 | 30篇 |
专业分类
儿科学 | 59篇 |
妇产科学 | 9篇 |
基础医学 | 50篇 |
口腔科学 | 11篇 |
临床医学 | 96篇 |
内科学 | 48篇 |
皮肤病学 | 3篇 |
神经病学 | 28篇 |
特种医学 | 19篇 |
外科学 | 33篇 |
综合类 | 9篇 |
预防医学 | 18篇 |
眼科学 | 13篇 |
药学 | 28篇 |
中国医学 | 1篇 |
肿瘤学 | 45篇 |
出版年
2021年 | 3篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2015年 | 10篇 |
2014年 | 7篇 |
2013年 | 8篇 |
2012年 | 8篇 |
2011年 | 15篇 |
2010年 | 10篇 |
2009年 | 8篇 |
2008年 | 14篇 |
2007年 | 9篇 |
2006年 | 15篇 |
2005年 | 12篇 |
2004年 | 10篇 |
2003年 | 12篇 |
2002年 | 9篇 |
2001年 | 15篇 |
2000年 | 8篇 |
1999年 | 15篇 |
1998年 | 14篇 |
1997年 | 9篇 |
1996年 | 12篇 |
1995年 | 11篇 |
1994年 | 7篇 |
1992年 | 11篇 |
1991年 | 19篇 |
1990年 | 13篇 |
1989年 | 8篇 |
1988年 | 22篇 |
1987年 | 15篇 |
1986年 | 14篇 |
1985年 | 15篇 |
1984年 | 10篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 11篇 |
1980年 | 3篇 |
1979年 | 9篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 10篇 |
1971年 | 6篇 |
1966年 | 2篇 |
1963年 | 2篇 |
1932年 | 2篇 |
排序方式: 共有470条查询结果,搜索用时 15 毫秒
1.
2.
C. W. Chow A. Poulos A. J. Fellenberg J. Christodoulou D. M. Danks 《Acta neuropathologica》1992,83(2):190-195
Summary Recognition of adrenal atrophy during a review of autopsy findings in two sisters who died at 8 months and 3 1/2 years prompted estimation of very long chain fatty acids, phytanic acid and pristanic acid on wet liver fixed in formalin for 12 years. These were shown to be markedly increased and defects in multiple peroxisomal functions and decrease in particulate catalase were shown in cultured fibroblasts, confirming an abnormality of peroxisomal biogenesis. The patients had presented with failure to thrive, recurrent diarrohea and vomiting, poor mental development, retinal pigmentation, blindness and in the older patient deafness, with only mild dysmorphic features. Autopsy in the older patient showed adrenal atrophy, cirrhosis, and foamy histiocytes in multiple organs. The brain showed no demyelination, little cytoarchitectural abnormality, occasional perivascular histiocytes in the grey matter and meninges and prominent Purkinje cells in the molecular layer of the cerebellum. In the younger patient the changes were very subtle in spite of the marked clinical similarity. Despite the young age at death the clinicopathological features are most suggestive of infantile Refsum disease. In many situations anatomical pathology can be very useful in the recognition and study of peroxisomal disorders. 相似文献
3.
Janine A Danks Patricia M W Ho Amanda J Notini Frosa Katsis Peter Hoffmann Bruce E Kemp T John Martin Jeffrey D Zajac 《Journal of bone and mineral research》2003,18(7):1326-1331
A PTH gene has been isolated from the fish Fugu rubripes. The encoded protein of 80 amino acid has the lowest homology with any of the PTH family members. Fugu PTH(1-34) had 5-fold lower potency than human PTH(1-34) in a mammalian cell system. INTRODUCTION: Parathyroid hormone (PTH) is the major hypercalcemic hormone in higher vertebrates. Fish lack parathyroid glands, but there have numerous attempts to identify and isolate PTH from fish. MATERIALS AND METHODS: Polymerase chain reaction (PCR) was performed with primers based on preliminary data from the Joint Genome Institute database. PCR amplification was performed on genomic DNA isolated from Fugu rubripes. PCR products were purified and DNA was sequenced. All sequence was confirmed from more than one independently amplified PCR product. Multiple sequence alignments were carried out, and the percentage of identities and similarities were calculated. An unrooted phylogenetic tree, using all the known PTH and PTH-related protein (PTHrP) amino acid sequences, was determined. Synthetic peptides were tested in a biological assay that measured cyclic adenosine 3',5'-monophosphate formation in UMR106.1 cells. Rabbit polyclonal antisera specific for N-terminal human PTHrP and one rabbit polyclonal antiserum specific for N terminus hPTH were used to test the cross-reactivity with fPTH(1-34) in immunoblots. 相似文献
4.
5.
D. M. Danks 《European journal of pediatrics》1991,150(3):142-148
There has been some limited progress in the understanding of the basic defect in Wilson disease and the gene concerned has been located to the chromosome region 13q14. Treatment with zinc has emerged as a definite alternative to penicillamine administration and some shortcomings and/or hazards of both forms of therapy have emerged as their modes of action have been studied more carefully. Tetrathimolybdate may have a place in treatment, especially when rapid complexing of copper is important. Hepatic copper accumulation occurs in a number of cholestatic diseases and they play an important part in pathogenesis and can occasionally lead to neurologic toxic effects. Copper overload in the newborn period when biliary excretion of copper is inefficient may establish a vicious cycle of copper accumulation and liver damage in Indian childhood cirrhosis and less frequently in babies in other countries. 相似文献
6.
In a prospective, randomized study of insemination with donor semen,
intracervical insemination by straw was compared with insemination using a
cervical cap with an intracervical reservoir. A total of 91 patients
completed 486 treatment cycles. There were no significant differences in
age, parity, indication for insemination by donor, or method of cycle
monitoring between women who became pregnant and those who did not conceive
with either insemination method. In 236 standard intracervical insemination
cycles, 14 patients became pregnant (5.9% per cycle), whereas 38 patients
conceived in 250 cervical cap cycles (15.2% per cycle). Both the crude
pregnancy rates and the cumulative pregnancy rates calculated by the
Kaplan-Meier life-table method were significantly different (chi(2)-test, P
< 0.001, and log-rank test, P < 0.005 respectively). Pregnancy rates
in artificial insemination with cryopreserved donor semen may be improved
by the use of a cervical cap when compared to cervical insemination by
straw. The use of the cervical cap may prolong the exposure of the
spermatozoa to the cervical mucus and prevent the backflow of semen into
the vagina.
相似文献
7.
Low-artifact intravascular devices: MR imaging evaluation 总被引:2,自引:0,他引:2
Teitelbaum GP; Ortega HV; Vinitski S; Stern H; Tsuruda JS; Mitchell DG; Rifkin MD; Bradley WG Jr 《Radiology》1988,168(3):713-719
Flow-phantom magnetic resonance (MR) imaging, with use of both spin-echo (SE) and gradient-echo (GRE) techniques at 1.5 T, was performed on the percutaneous Greenfield (beta-III titanium alloy [TMA wire]), Amplatz (MP32-N alloy), and Simon nitinol filters and TMA wire facsimiles of the bird's nest, Gunther, new retrievable, and Amplatz vena caval filters. SE imaging allowed detection of thrombi as small as 5 X 5 mm trapped within the percutaneous Greenfield, Simon nitinol, and TMA-wire facsimile filters; with the MP32-N Amplatz filter, a larger volume of thrombus (10 X 20-mm clots) was necessary for clot detection. GRE imaging allowed detection of intraluminal tilting of the percutaneous Greenfield and facsimile Amplatz (TMA-wire) filters. GRE imaging was useful for demonstrating postfilter turbulence due to clots, which was greatest for the Amplatz filter. Imaging of facsimile vascular devices made of tantalum or TMA wire did not cause the severe "black-hole" MR artifacts typical of the stainless-steel devices. SE and GRE imaging were very useful for determining caval patency in two patients with previously placed Mobin-Uddin filters. Noninvasive MR evaluation of blood vessels in the presence of a variety of low-artifact intravascular devices appears feasible. 相似文献
8.
9.
Immunohistochemical localization of parathyroid hormone-related protein in parathyroid adenoma and hyperplasia 总被引:3,自引:0,他引:3
J A Danks P R Ebeling J A Hayman H Diefenbach-Jagger F M Collier V Grill J Southby J M Moseley S T Chou T J Martin 《The Journal of pathology》1990,161(1):27-33
Parathyroid hormone-related protein (PTHrP) is invoked as the cause of humoral hypercalcaemia of malignancy (HHM); it is contained in the keratinocyte layer of normal skin; and there is evidence that is is produced by fetal parathyroids. Antibodies against synthetic PTHrP peptides have been raised in rabbits and sheep. This immunohistochemical study has found that primary parathyroid adenomata and hyperplastic glands from patients with chronic renal failure stain positively with antisera against PTHrP(1-34) and PTHrP(50-69). Primary hyperplastic glands are negative. No staining with anti-PTHrP(106-141) antiserum could be detected immunohistochemically in any of the parathyroid adenomata or hyperplasia. 相似文献
10.
Monoclonal antibodies to glycoproteins of Vinca alkaloid-resistant human leukemic cells 总被引:6,自引:0,他引:6
Drug resistance is a major problem in the successful treatment of cancer. Resistance to one drug is often associated with cross-resistance to other anticancer agents. This is commonly seen with the "natural product" anticancer drugs such as the Vinca alkaloids and anthracyclines. In experimental systems, specific changes in plasma membranes characterize this "multiple drug resistance." The most prominent of these is the enhanced expression in several systems of high-molecular-weight glycoproteins ranging from Mr approximately equal to 150,000 to approximately equal to 180,000, the amount of which has been shown to be related to the degree of drug resistance. We report here the production of three monoclonal antibodies that bind preferentially to the surfaces of cultured human leukemic lymphoblasts resistant to the Vinca alkaloid vinblastine. Each antibody recognizes a surface membrane glycoprotein with molecular weights of 180,000 to 210,000. Additionally, two of the antibodies also recognize a second surface glycoprotein with molecular weights of either approximately equal to 155,000 or approximately equal to 130,000. All of these glycoproteins are overexpressed in the alkaloid-resistant cells. While a Mr approximately equal to 180,000 protein has been shown to be associated with multiple drug resistance, the other two glycoproteins have not been described previously in these cells. These antibodies may be useful in studies of the mechanisms of drug resistance, as well as in screening cells from drug-resistant patients for these resistance-associated glycoproteins. 相似文献