Characterization and Mosquitocidal Potency of Bacillus thuringiensis Isolate SB1 from the Sundarbans Mangrove,West Bengal,India

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences - Anopheles, Culex and Aedes mosquitoes are serious disease vectors in India including the malaria endemic...     

Characterization and Mosquitocidal Potential of the Soil Bacteria <Emphasis Type="Italic">Aneurinibacillus aneurinilyticus</Emphasis> Isolated from Burdwan,West Bengal,India

Aedes aegypti, Anopheles subpictus and Culex quinquefasciatus are the principal vector mosquitoes globally, especially in tropical and subtropical countries. It is very essential to control the mosquito population to prevent mosquito-borne disease transmission. The major goal of the present study was to isolate an alternative for the microbial mosquito control agents like Bacillus thuringiensis and Bacillus sphaericus. The spore forming soil bacteria isolated from Burdwan, West Bengal, India were assessed for mosquitocidal potential. One potent mosquitocidal isolate was characterized by phenotypic, biochemical and 16S rDNA sequence (GU244410) analysis. The isolate was identified as Aneurinibacillus aneurinilyticus. Five ml bacterial suspension of A. aneurinilyticus (22 × 10⁹ colony forming unit/milliliter)/100 ml water resulted in 34, 59.6 and 100 % mortality of Ae. aegypti, Cx. quinquefasciatus and An. subpictus larvae respectively within 72 h, whereas, 10 ml suspension having same concentration showed 95 and 97 % death of Ae. aegypti and Cx. quinquefasciatus larvae respectively within the same time period.