全文获取类型
收费全文 | 607篇 |
免费 | 68篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 21篇 |
妇产科学 | 8篇 |
基础医学 | 118篇 |
口腔科学 | 15篇 |
临床医学 | 45篇 |
内科学 | 81篇 |
皮肤病学 | 12篇 |
神经病学 | 22篇 |
特种医学 | 107篇 |
外科学 | 102篇 |
综合类 | 3篇 |
预防医学 | 10篇 |
眼科学 | 6篇 |
药学 | 29篇 |
中国医学 | 1篇 |
肿瘤学 | 87篇 |
出版年
2023年 | 13篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 5篇 |
2018年 | 13篇 |
2017年 | 18篇 |
2016年 | 15篇 |
2015年 | 16篇 |
2014年 | 24篇 |
2013年 | 33篇 |
2012年 | 23篇 |
2011年 | 20篇 |
2010年 | 18篇 |
2009年 | 27篇 |
2008年 | 19篇 |
2007年 | 27篇 |
2006年 | 33篇 |
2005年 | 22篇 |
2004年 | 19篇 |
2003年 | 29篇 |
2002年 | 16篇 |
2001年 | 21篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 20篇 |
1997年 | 15篇 |
1996年 | 15篇 |
1995年 | 6篇 |
1994年 | 13篇 |
1993年 | 8篇 |
1992年 | 6篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 16篇 |
1988年 | 14篇 |
1987年 | 6篇 |
1986年 | 12篇 |
1985年 | 11篇 |
1984年 | 8篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1980年 | 3篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1970年 | 4篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1965年 | 2篇 |
排序方式: 共有677条查询结果,搜索用时 726 毫秒
1.
2.
3.
Paolo Cossu Rocca Matteo Brunelli Stefano Gobbo Albino Eccher Emma Bragantini Maria M Mina Vincenzo Ficarra Filiberto Zattoni Alberto Zamò Maurizio Pea Aldo Scarpa Marco Chilosi Fabio Menestrina Franco Bonetti John N Eble Guido Martignoni 《Modern pathology》2007,20(7):722-728
S100A1 is a calcium-binding protein, which has been recently found in renal cell neoplasms. We evaluated the diagnostic utility of immunohistochemical detection of S100A1 in 164 renal cell neoplasms. Forty-one clear cell, 32 papillary, and 51 chromophobe renal cell carcinomas, and 40 oncocytomas, 164 samples of normal renal parenchyma adjacent to the tumors and 13 fetal kidneys were analyzed. The levels of S100A1 mRNA detected by quantitative RT-PCR analysis of frozen tissues from seven clear cell, five papillary, and six chromophobe renal cell carcinomas, four oncocytomas, and nine samples of normal renal tissues adjacent to neoplasms were compared with the immunohistochemical detection of protein expression. Clear cell and papillary renal cell carcinomas showed positive reactions for S100A1 in 30 out of 41 tumors (73%) and in 30 out of 32 (94%) tumors, respectively. Thirty-seven renal oncocytomas out of 40 (93%) were positive for S100A1, whereas 48 of 51 (94%) chromophobe renal cell carcinomas were negative. S100A1 protein was detected in all samples of unaffected and fetal kidneys. S100A1 mRNA was detected by RT-PCR in all normal kidneys and renal cell neoplasms, although at very different levels. Statistical analyses comparing the different expression of S100A1 in clear cell and chromophobe renal cell carcinomas observed by immunohistochemical and RT-PCR methods showed significant values (P<0.001), such as when comparing by both techniques the different levels of S100A1 expression in chromophobe renal cell carcinomas and oncocytomas (P<0.001). Our study shows that S100A1 protein is expressed in oncocytomas, clear cell and papillary renal cell carcinomas but not in chromophobe renal cell carcinomas. Its immunodetection is potentially useful for the differential diagnosis between chromophobe renal cell carcinoma and oncocytoma. Further, S100A1 protein expression is constantly detected in the normal parenchyma of the adult and fetal kidney. 相似文献
4.
In vitro and in vivo precipitation of iodinated contrast agents when ioxaglate and papaverine are given together has been reported. To verify these reports and to investigate other medications not previously tested, the authors analyzed mixtures of contrast agents and medications in vitro with a light spectrophotometer and observed them for visible precipitates for up to 120 minutes. Previously reported incompatibilities between ionic or low-osmolality contrast media and medications were verified, and several new incompatibilities were discovered. No incompatibilities were found when the drugs tested were mixed with the new nonionic contrast media. 相似文献
5.
Sequential contrast-enhanced MR imaging of the penis 总被引:1,自引:0,他引:1
6.
Matteo Brunelli John N Eble Shaobo Zhang Guido Martignoni Liang Cheng 《Modern pathology》2003,16(10):1053-1059
It has been suggested that gains of chromosomes 7 and 17 and loss of Y occur in renal papillary adenoma and that progression to papillary renal cell carcinoma is marked by gains of additional chromosomes, most frequently 12, 16, and 20. Previous studies have included very few lesions of <5 mm in diameter, a requirement of the present definition of papillary adenoma. Ten papillary adenomas (ranging from 1 to 5 mm in diameter) from autopsy material and 10 surgically resected papillary renal cell carcinomas were studied with fluorescence in situ hybridization in paraffin sections using centromeric probes for chromosomes 7, 12, 16, 17, 20, and Y diluted 1:100 with tDenHyb1 buffer. The signals in 50 to 150 nuclei were counted in each tumor. Controls for all the probes were normal renal tissues from the same patients. Three or more signals per nucleus were frequently observed in papillary adenomas: chromosome 7 (range, 10 to 50%; > or = 30% in 9 of 10), 17 (range, 10 to 47%; > or = 30% in 7), 16 (range, 1 to 63%; > or = 10% in 5), 12 (range, 0 to 32%; > or =10% in 4), and 20 (range, 5 to 49%; > or = 10% in 5). Loss of the Y chromosome was observed in 80 to 90% of nuclei in 9 adenomas from males. Three or more signals were frequent in papillary renal cell carcinomas: chromosome 7 (range, 32 to 63%; > or =30% in 10 of 10), 17 (range, 28 to 61%; > or = 30% in 7), 16 (range, 0 to 45%; > or = 10% in 6), 12 (range, 1 to 37, > or = 10% in 5), 20 (range, 2 to 44%; > or = 10% in 4). No signal for Y was observed in 12 to 88% (> or = 81% in 6) of nuclei in 7 carcinomas from males. Statistical analysis showed no difference between adenomas and carcinomas. Gains of chromosomes 7, 17, 16, 12, and 20 and loss of the Y chromosome occur early in the evolution of papillary renal cell neoplasia in tumors that are only a few millimeters in diameter. Progressive gains of these chromosomes do not appear to correlate with the transition from adenoma to carcinoma. 相似文献
7.
Chlamydial etiology of acute lower respiratory tract infections in children in the Sudan 总被引:2,自引:0,他引:2
B Herrmann MAM Salih BE Yousif O Abdelwahab P-A Mårdh 《Acta paediatrica (Oslo, Norway : 1992)》1994,83(2):169-172
The role of Chlamydia pneumoniae in 110 Sudanese children with signs of acute lower respiratory tract infections (ALRI) was investigated. Four (3.6%) had evidence of C. pneumoniae infection, of whom 3 were culture-positive, while 1 had an antibody response suggesting a recent infection. IgG antibodies at a titer of ≥1:32 to C. pneumoniae, Chlamydia psittaci and Chlamydia trachomatis were detected in 27 (24.5%), 27 (24.5%) and 7 (6.4%) of the 110 ALRI cases, respectively. C. pneumoniae, C. trachomatis or C. psittaci were not detected in nasopharyngeal secretions from any of 110 patients when fluorescence-labeled specific monoclonal antibodies were used. In a seroepidemiological survey, 318 healthy Sudanese persons aged between 1 month and 67 years were studied for C. pneumoniae antibodies. 相似文献
8.
A panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. The lectins were derived from Lotus tetragonolobus, Glycine max, Dolichos biflorus, and Arachis hypogaea. Two patterns predominated: one of staining with the lectins of D biflorus and G max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of L tetragonolobus, suggesting proximal tubular differentiation. 相似文献
9.
P. VON DEN DRIESCH C. STEFFAN A. ZÖBE O.P. HORNSTEIN 《Clinical and experimental dermatology》1994,19(3):274-277
We report the case of a 39-year-old female patient suffering from Sweet's syndrome after an upper respiratory tract infection. Cyclosporin A at a dose of 10 mg/kg per day was given as initial treatment. Skin lesions and general malaise resolved within 9 days. The cyclosporin dose was decreased within 21 days, without recurrence of the eruption. Cyclosporin is a potent inhibitor of T lymphocytes, but affects granulocyte and monocyte functions as well. Success of treatment in our case shows that cyclosporin represents an alternative to steroid treatment in patients with Sweet's syndrome. 相似文献
10.