宁夏枸杞无公害生产关键技术研究与示范

分析无公害枸杞生产的相关因子，研究枸杞无公害生产关键技术，并进行示范与推广：枸杞产品已基本符合无公害食品的部颁标准。     

Growth of Pakistani children in relation to the 1990 growth standards

This study was designed to compare the growth of Pakistani schoolchildren in the UK with the 1990 UK growth standards. Measurements of height, weight, and sitting height were performed on 785 Pakistani schoolchildren aged 5-14 years with the mean values for each age and sex being plotted on the UK growth standards. The results were expressed as SD scores relative to the 1990 reference data. The mean height for the boys was only 0.2 SD scores below the mean for the new growth standards with the mean height for the girls being 0.4 SD scores below the mean. The mean values for weight and body mass index were 0.3 and 0.5 SD scores less than the mean for boys and girls respectively. This study demonstrates that the growth of Pakistani schoolchildren in the UK is comparable to the 1990 UK growth standards with only minor differences. It is not safe to assume that short stature or low body weight in a Pakistani child is due to his or her ethnic background.     

MAPK和MPF对小鼠受精卵有丝分裂期作用

目的探讨有丝分裂促进因子（MPF）和丝裂原活化的蛋白激酶（MAPK）在小鼠受精卵第一有丝分裂期（M期）中的作用及相互关系.方法分别用MAPK激酶特异性抑制剂（U0126）和MPF特异性抑制剂（roscovitine）抑制受精卵细胞MAPK和MPF的活性,通过同位素测定MAPK和MPF的活性变化,利用显微镜进行形态观察来判定MAPK活性变化对受精卵发育的影响.结果在受精卵第一次有丝分裂期,正常组小鼠受精卵MAPK和MPF的活性均有短暂升高.在U0126处理组,MAPK的活性受抑制会导致受精卵处于M期而不发生分裂,但MPF的活性未受到影响.在roscovitine处理组,MPF的活性受到抑制导致受精卵处于M期不发生分裂,此时MAPK不发生活化.结论 MAPK和MPF的活化对于小鼠受精卵完成有丝分裂是不可缺少的,在有丝分裂期MPF参与MAPK的活化.     

高强度聚焦超声治疗的麻醉处理

高强度聚焦超声是一种无创的高温治疗肿瘤方法,其治疗时需要麻醉,本文就其治疗肿瘤的机制、麻醉特点以及目前国内外针对不同部位肿瘤实施的麻醉方法作一总结。     

Determination of malondialdehyde-induced DNA damage in human tissues using an immunoslot blot assay

Malondialdehyde (MDA) is a product of lipid peroxidation and prostaglandin biosynthesis. It is mutagenic and carcinogenic and the major adduct formed by reaction with DNA, a highly fluorescent pyrimidopurinone (M1-dG), has been detected in healthy human liver and leukocyte DNA. Analytical methods used so far for the detection of M1- dG have not been applied to a large number of individuals or variety of samples. Often, only a few microg of DNA from human tissues are available for analysis and a very sensitive assay is needed in order to detect background levels of M1-dG in very small amounts of DNA. In this paper, the development of an immunoslot blot (ISB) assay for the measurement of MI-dG in 1 microg of DNA is described. The limit of detection of the assay is 2.5 adducts per 10(8) bases. A series of human samples were analysed and levels of 5.6-9.5 (n = 8) and 3.1-64.3 (n = 42) of M1-dG per 10(8) normal bases were detected in white blood cell and gastric biopsy DNA, respectively. Results on four human samples were compared with those obtained using an HPLC/32P-post- labelling (HPLC/PPL) method previously developed and indicated a high correlation between M1-dG levels measured by the two assays. The advantages of ISB over other assays including HPLC/PPL, such as the possibility of analysing 1 microg DNA/sample and the fact that it is less time-consuming and laborious, means that it can be more easily used for routine analysis of a large number of samples in biomonitoring studies.      

监测麻醉用于门诊隆胸术的观察

目的:观察监测麻醉(MAC)用于门诊隆胸术是否优于传统的区域阻滞麻醉.方法:32例受术者分为MAC组和对照组(Con),MAC组给予芬太尼、异丙酚后行区域阻滞麻醉;对照组行传统的区域阻滞麻醉.结果:麻醉效果:MAC组优良率高于Con组P＜0.01;MAP和HR的改变:MAC组P>0.05;Con组术中高于术前P＜0.05;呼吸:两组的SpO2最低值比较P>0.05;局麻药毒性反应:Con组高于MAC组P＜0.01;术毕至离院时间:PADSS评分≥9分,所需的时间≥120min,两组均无1例.结论:MAC用于门诊隆胸术优于传统的区域阻滞法.     

舒芬太尼、芬太尼用于胸外手术麻醉的临床比较

 目的 评价舒芬太尼用于胸外科手术时对血流动力学、术后苏醒时间及血浆中应激物质(去甲肾上腺素、肾上腺素)浓度的影响.方法 择期行胸外科手术患者30例,ASAⅠ～Ⅱ级,随机分为舒芬太尼组(S组,n=16 )和芬太尼组(F组,n=14),于手术各时间点分别监测血流动力学指标,比较术后苏醒时间和镇痛效果,并测定各时点血浆中的肾上腺素、去甲肾上腺素浓度.结果 F组血流动力学变化比S组大(P <0.05),S组术后苏醒时间短于F组,但镇痛时间长于F组,S组血浆中的肾上腺素、去甲肾上腺素浓度变化较小.结论 舒芬太尼用于长时间的胸外手术中,可维持良好的血流动力学稳定性,苏醒较快,但镇痛效果维持时间长,体内应激性物质产生较少.     

Hemorrhagic tumor necrosis during a pilot trial of tumor necrosis factor-alpha and anti-GD3 ganglioside monoclonal antibody in patients with metastatic melanoma

Hemorrhagic tumor necrosis is an inflammatory event that leads to selective destruction of malignant tissues, with both potentially toxic and beneficial consequences. A pilot clinical trial was undertaken combining tumor necrosis factor-alpha (TNF-alpha) with the monoclonal antibody R24 (MoAb R24) against GD3 ganglioside in patients with metastatic melanoma. Patients received MoAb R24 to recruit leukocytes to the tumor followed by low doses of recombinant TNF-alpha to activate leukocytes. Eight patients were treated and seven patients had mild toxicity. One patient with extensive metastatic melanoma developed tumor lysis syndrome within hours after treatment with almost complete necrosis of bulky tumors in multiple visceral sites. To our knowledge, this is the first documented case of hemorrhagic tumor necrosis in a patient with metastatic cancer in multiple visceral sites.