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1.
Wesley H. Self MD MPH Joyce Mickanin MSN RN Carlos G. Grijalva MD MPH Freda H. Grant MT ASCP Michelle C. Henderson MSN RN Glenda Corley MT ASCP D. Glen Blaschke II MD Candace D. McNaughton MD MPH Tyler W. Barrett MD MSCI Thomas R. Talbot MD MPH Barbara R. Paul MD 《Academic emergency medicine》2014,21(3):274-282
2.
Dr. Michael D. Schuffler MD Lawrence R. Kaplan MD Linda Johnson BS MT ASCP 《Digestive diseases and sciences》1978,23(9):821-828
The purpose of this investigation was to determine the frequency and severity of small intestinal mucosal damage in pseudoobstruction syndromes. One hundred eighty-nine interpretable biopsies from 12 patients were blindly reviewed by two investigatiors. The underlying disorders were scleroderma in 7 and idiopathic intestinal pseudoobstruction in 5. All 12 had small-intestinal dilatation on small-bowel series. Eight of the 12 patients had biopsies characterized by moderate, to severe mucosal damage; 3 of these had some biopsies which were flat. The damage did not correlate with: (1) types and numbers of organisms recovered from small intestinal aspirates; (2) duration of illness; (3) degree of dilatation of the proximal small bowel; (4) concentrations of deconjugated bile salts in small intestinal fluid; or (5) amount of fat absorbed in fat-balance studies. We conclude that mucosal damage is common in pseudoobstruction syndromes. The pathogenesis of the damage and its relationship to intraluminal bacteria remain undefined. 相似文献
3.
Gina Shetty BS Georgia M. Beasley MD Sara Sparks BS MT ASCP Michael Barfield MD Melanie Masoud BS Paul J. Mosca MD PhD Scott K. Pruitt MD PhD April K. S. Salama MD Cliburn Chan MD PhD Douglas S. Tyler MD Kent J. Weinhold PhD 《Annals of surgical oncology》2013,20(4):1128-1135
Background
Preprocedure clinical and pathologic factors have failed to consistently differentiate complete response (CR) from progressive disease (PD) in patients after isolated limb infusion (ILI) with melphalan for unresectable in-transit extremity melanoma.Methods
Multiplex immunobead assay technology (Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel, Millipore Corp., Billerica, MA; and Magpix analytical test instrument, Luminex Corp., Austin, TX) was performed on pre-ILI plasma to determine concentrations of selected cytokines (MIP-1α, IL-1Rα, IP-10, IL-1β, IL-1α, MCP-1, IL-6, IL-17, EGF, IL-12p40, VEGF, GM-CSF, and MIP-1β) on a subset of patients (n = 180) who experienced CR (n = 23) or PD (n = 24) after ILI. Plasma from normal donors (n = 12) was also evaluated.Results
Of 180 ILIs performed, 28 % (95 % confidence interval 22–35, n = 50) experienced a CR, 14 % (n = 25) experienced a partial response, 11 % (n = 21) had stable disease, 34 % (n = 61) had PD, and 13 % (n = 23) were not evaluable for response. Tumor characteristics and pharmacokinetics appeared similar between CR (n = 23) and PD (n = 24) patients who underwent cytokine analysis. Although there were no differences in cytokine levels between CR and PD patients, there were differences between the melanoma patients and controls. MIP-1α, IL-1Rα, IL-1β, IL-1α, IL-17, EGF, IL-12p40, VEGF, GM-CSF, and MIP-1β were significantly higher in normal controls compared to melanoma patients, while IP-10 was lower (p < 0.001) in controls compared to melanoma patients.Conclusions
Patients with unresectable in-transit melanoma appear to have markedly decreased levels of immune activating cytokines compared to normal healthy controls. This further supports a potential role for immune-targeted therapies and immune monitoring in patients with regionally advanced melanoma. 相似文献4.
Sharon L. Lewis PhD RN FAAN Denise Miner‐Williams PhD RN CHPN Allen Novian PhD LMFT LPC Monica I. Escamilla MS PsyD Paula H. Blackwell MT ASCP Jennifer Hale Kretzschmar BBS Lyda C. Arévalo‐Flechas PhD RN Peter N. Bonner MS 《Rehabilitation nursing》2009,34(4):151-159
Aging baby boomers, longer life spans, and rising levels of Alzheimer's disease and related dementias (ADRD) will result in a caregiver crisis in the near future. The ways in which caregivers deal with stresses related to caregiving will be critical to both their own well‐being and their ability to care for others. The purpose of this article is to describe the Stress‐Busting Program (SBP) for family caregivers and its effectiveness. The essential components of the SBP are education, stress management, problem solving, and support delivered in a group setting for 9 weeks. Results of the SBP indicate that throughout the program, caregivers experienced significant improvements in general health, vitality, social function, and mental health scores and decreases in anxiety, anger/hostility, depression, perceived stress, and caregiver burden. The SBP is a cost‐effective health‐promotion strategy for caregivers who have substantial ongoing stress. 相似文献
5.
6.
Odile David MD Robert J. Cabay MD Seema Pasha MD Ruth Dietrich ASCP Lu Leach BS Meihua Guo MD Swati Mehrotra MD 《Cancer cytopathology》2009,117(3):157-166
BACKGROUND:
Discordant results of cervical biopsy histology after a cytologic diagnosis of high‐grade squamous intraepithelial lesion (HSIL) are often attributed to sampling variation. The purpose of the current study was to determine whether deeper levels and ancillary staining (p16Ink4a and ProExC) reduce the discordant rate.METHODS:
A total of 246 cases of HSIL were retrieved from the computerized database from 2005 and 2006. Of these cases, 151 were followed by cervical biopsy. There was cytologic‐histologic correlation in 87 cases, as defined by the presence of high‐grade (2 or 3) cervical intraepithelial neoplasia (HGCIN). For each discordant biopsy (n = 64), 2 deeper levels for hematoxylin and eosin (H&E) were taken at 30‐μ and 90‐μ depths, and 4 sections for p16Ink4a and ProExC staining were taken at a 60‐μ depth. All cytologic and histologic material from these 64 cases was reviewed by 3 cytopathologists. In 2 cases, the original HSIL diagnoses were downgraded and the cases censored from the study.RESULTS:
Fifty‐seven of the 62 discordant cases had sufficient tissue for deeper levels and ancillary staining. Two of 57 cases were reclassified to HGCIN. In both of these cases, reclassification was suggested by results of immunostains; however, the H&E sections were necessary for definitive interpretation of the immunostain results.CONCLUSIONS:
In the current study, deeper levels and ancillary staining with p16Ink4a and ProExC did not significantly reduce the discordance rate. Although there are many known causes of sampling variation, including factors related to colposcopic technique, regression of infection, and insufficient histologic sectioning, sampling variation remains a valid justification of noncorrelation in women with HSIL followed up by cervical biopsy alone. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society. 相似文献7.
Mario Fernando Muñoz-Guerra MD PhD María Encarnación Fernández-Contreras PhD Ana Laura Capote Moreno MD Irene Domínguez Martín ASCP Belén Herráez ASCP Carlos Gamallo MD PhD 《Annals of surgical oncology》2009,16(8):2351-2358
Background Hypoxia-inducible factor-1 (HIF-1) is the key regulator of cellular responses to hypoxia and presumably plays a central role
in the control of tumor growth. Polymorphisms or mutations increasing its activity and stability in vitro under normoxia have
recently been identified. In this study, we aimed to investigate the effect of C1772T and G1790A single nucleotide polymorphisms
(SNPs), located within the exon 12 of HIF-1α on the prognosis of early stages of oral squamous cell carcinoma (OSCC).
Methods The frequency of C1772T and G1790A polymorphisms was determined by PCR-RFLP in 139 DNA samples from healthy volunteers and
74 patients with surgically treated T1/2 N0 OSCC. The impact of HIF-1α SNPs on tumor size, invasive depth, pathological features,
and histological grade was studied. Correlations between genotype and relapse and/or disease-specific survival were evaluated
by Kaplan-Meier analysis and log-rank test.
Results Concerning G1790A SNP, the frequencies of GA heterozygous and AA variant homozygous genotypes were significantly higher in
patients than in healthy volunteers (32.8% vs. 6.5% and 4.7% vs. none, respectively) (P < .0001). Also, the presence of the variant allele A was associated to disease-relapse (P = .02) and shorter disease-free survival (P = .04). The genotype distribution of C1772T did not diverge between patients and healthy subjects, and no differences were
observed with respect to disease-free or overall survival.
Conclusions Our results suggest that G1790A polymorphism in the HIF-1α gene might confer susceptibility to OSCC and could be a marker
of disfavorable prognosis at early stages. 相似文献
8.
Vita Sullivan BA Blanche Berger BA ASCP Robert W. Wissler PhD MD The PDAY Research Group 《Cardiovascular pathology》1996,5(5):259
Standardized postmortem samples of thoracic and abdominal aortas from traumatic death victims (aged 15–34 inclusive) were selected according to renal indices of estimated blood pressure. Half of the males had renal small-artery evidence of elevated blood pressure, and half did not. The group consisted of an approximately equal number of black and white males. All of the individuals were nonsmokers and had similar age, cholesterol, and HDL distribution. Lipid deposition in the thoracic and abdominal aorta sections was determined quantitatively by means of computer micromorphometry in sections stained with Oil Red O. Results showed that there is a marked increase in extracellular lipid deposition in the intima for those arteries studied with elevated renal indices of hypertension. In addition, there is significantly more extracellular lipid in the abdominal aortas in black males than in white males. Also notable was the finding that the thoracic aorta samples exhibited significantly thicker intimas and larger intimal areas in the high blood pressure index groups than in the low blood pressure index groups. These results suggest that the development of atherosclerotic lesions may be due to an increased deposition of extracellular lipid in the matrix of the arterial intima. 相似文献
9.
Rusmir Feratovic CT ASCP Sharyn N. Lewin MD Yukio Sonoda MD Kay J. Park MD Nadeem R. Abu‐Rustum MD Andre L. Moreira MD PhD Oscar Lin MD PhD 《Cancer cytopathology》2008,114(1):1-6
BACKGROUND.
Radical trachelectomy is a surgical procedure intended to preserve fertility in patients with early‐stage cervical carcinoma in which the cervix is amputated in continuity with the parametrium and upper vagina, thereby sparing the uterus and adnexa. Follow‐up is performed with periodic cytology specimens. The objective of the current study was to analyze the cytologic findings after this novel procedure.METHODS.
Isthmic and vaginal Papanicolaou‐stained ThinPrep cytology specimens taken from patients after radical vaginal and abdominal trachelectomy were reviewed. The specimens were also analyzed for the presence of benign endocervical cells, lower uterine segment glandular cells, endometrial stromal cells, and endometrial cells. The findings were correlated with the original diagnosis and follow‐up, which included subsequent cytology specimens and biopsies.RESULTS.
Cytology specimens (n = 223) from 44 patients were included in this study. An endometrial component was identified in 131 of the cases (59%). Twenty‐eight cases were diagnosed as abnormal in the original cytology examination. Twenty of these cases and 5 additional cases that were diagnosed cytologically as benign had subsequent biopsies. The biopsies confirmed the presence of a lesion in only 4 of 25 biopsies (3 low‐grade squamous intraepithelial lesions and 1 adenosquamous carcinoma). All cases diagnosed as atypical glandular cells represented tubal metaplasia, lower uterine segment glandular cells, or endometrial stromal cells.CONCLUSIONS.
Cytology specimens after trachelectomy frequently contain glandular cells from the lower uterine segment epithelium or endometrial stromal cells, which can lead to a misdiagnosis of atypical glandular cells of undetermined significance. Tubal metaplasia is also a potential pitfall in these specimens. Pathologists and gynecologic oncologists should be aware of the potential pitfalls in the surveillance of smears after trachelectomy. Cancer (Cancer Cytopathol) 2008. © 2007 American Cancer Society. 相似文献10.
Xiomara Q. Rosales MD Vinod Malik PhD Amita Sneh PhD Lei Chen MS Sarah Lewis HT ASCP Janaiah Kota PhD Julie M. Gastier‐Foster PhD Caroline Astbury PhD Rob Pyatt PhD Shalini Reshmi PhD Louise R. Rodino‐Klapac PhD K. Reed Clark PhD Jerry R. Mendell MD Zarife Sahenk MD PhD 《Muscle & nerve》2013,47(5):731-739
Introduction: Recent in vitro studies suggest that CAPN3 deficiency leads initially to accelerated myofiber formation followed by depletion of satellite cells (SC). In normal muscle, up‐regulation of miR‐1 and miR‐206 facilitates transition from proliferating SCs to differentiating myogenic progenitors. Methods: We examined the histopathological stages, Pax7 SC content, and muscle‐specific microRNA expression in biopsy specimens from well‐characterized LGMD 2A patients to gain insight into disease pathogenesis. Results: Three distinct stages of pathological changes were identified that represented the continuum of the dystrophic process from prominent inflammation with necrosis and regeneration to prominent fibrosis, which correlated with age and disease duration. Pax7‐positive SCs were highest in the fibrotic group and correlated with down‐regulation of miR‐1, miR‐133a, and miR‐206. Conclusions: These observations, and other published reports, are consistent with microRNA dysregulation leading to inability of Pax7‐positive SCs to transit from proliferation to differentiation. This results in impaired regeneration and fibrosis. Muscle Nerve 47: 731–739, 2013 相似文献